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Citations & References (48)
Invitrogen™
Low Density Lipoprotein from Human Plasma (LDL)
Human low-density lipoprotein (LDL) is large protein complex (∼500,000 Da) that binds to a specific receptor on the surface ofRead more
| Catalog Number | Quantity |
|---|---|
| L3486 | 200 μL |
Catalog number L3486
Price (MXN)
-
Quantity:
200 μL
Human low-density lipoprotein (LDL) is large protein complex (∼500,000 Da) that binds to a specific receptor on the surface of vertebrate cells and delivers cholesterol via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon. These experiments are typically performed by adding fluorescently labeled LDL to cultured cells and analyzing them by microscopy or flow cytometry. Alternatively the fluorescently labeled LDL can be injected into test animals, and the distribution of the label can be analyzed after the specified time period. We offer an unlabeled LDL and two classes of labeled LDLs: those containing an unmodified apoprotein (used to study normal cholesterol delivery and internalization) and those with an acetylated (Ac) apoprotein (used to study cell types that express receptors specific for this acetylated version (i.e., endothelial and microglial cells)).
LDL Specifications:
• Label (Ex/Em): Unlabeled
• Acetylated: No
• Amount: 200 μL (2.5 mg/mL)
Fresh LDL Produced Continually
We prepare our LDL and AcLDL products from fresh human plasma approximately every two months. The nonacetylated LDL products are shipped within two weeks of their preparation. All acetylated LDL products are available on a continuous basis.
Nonacetylated vs. Acetylated LDL
LDL containing an unmodified apoprotein is used to study normal cholesterol delivery and internalization. If the lysine residues of LDL’s apoprotein have been acetylated, the LDL complex no longer binds to the LDL receptor, but rather is taken up by endothelial and microglial cells that possess “scavenger” receptors specific for that modified form.
Key Applications for Labeled LDL
Some of the many applications for labeled LDL complexes include:
• Counting cell-surface LDL receptors, and analyzing their motion and clustering following internalization
• Quantitating LDL receptor activity in fibroblasts (replacing the radiolabeled LDL assay)
• Investigating LDL expression and identifying LDL receptor deficiencies in various cell lines
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
LDL Specifications:
• Label (Ex/Em): Unlabeled
• Acetylated: No
• Amount: 200 μL (2.5 mg/mL)
Fresh LDL Produced Continually
We prepare our LDL and AcLDL products from fresh human plasma approximately every two months. The nonacetylated LDL products are shipped within two weeks of their preparation. All acetylated LDL products are available on a continuous basis.
Nonacetylated vs. Acetylated LDL
LDL containing an unmodified apoprotein is used to study normal cholesterol delivery and internalization. If the lysine residues of LDL’s apoprotein have been acetylated, the LDL complex no longer binds to the LDL receptor, but rather is taken up by endothelial and microglial cells that possess “scavenger” receptors specific for that modified form.
Key Applications for Labeled LDL
Some of the many applications for labeled LDL complexes include:
• Counting cell-surface LDL receptors, and analyzing their motion and clustering following internalization
• Quantitating LDL receptor activity in fibroblasts (replacing the radiolabeled LDL assay)
• Investigating LDL expression and identifying LDL receptor deficiencies in various cell lines
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration2.5 mg⁄ml
Detection MethodFluorescence
Dye TypeUnlabeled
Quantity200 μL
Shipping ConditionWet Ice
Product TypeSupplement
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C).
Frequently asked questions (FAQs)
If the Low Density Lipoprotein (LDL) from Human Plasma was accidentally frozen, can I still use it?
Can I use Low Density Lipoprotein (LDL) from Human Plasma on other species?
Citations & References (48)
Citations & References
Abstract
Fluorescent labeling of lipoproteins.
Journal:Methods Enzymol
PubMed ID:3724556
Molecular counting of low-density lipoprotein particles as individuals and small clusters on cell surfaces.
Journal:Biophys J
PubMed ID:3719072
'We employ the intensely fluorescent analogue diI-LDL (Barak, L. S., and W. W. Webb, 1981, J. Cell Biol. 90:595-604) as a counting marker to determine the numbers of LDL-receptor complexes that are contained in clusters on the surfaces of human fibroblasts and human epidermoid carcinoma cells. The application of quantitative
Receptor-mediated transcytosis of transferrin across the blood-brain barrier.
Journal:J Neurosci Res
PubMed ID:3694713
'The perfusion of rat brain with 125I-transferrin resulted in a receptor-mediated uptake of transferrin into the endothelium of the blood-brain barrier followed by its detection in the brain. During a pulse-chase experiment, 125I-transferrin accumulated in the endothelial cells during the pulse, with a decrease of this intraendothelial radioactivity during the
Uptake and trafficking of liposomes to the endoplasmic reticulum.
Journal:FASEB J
PubMed ID:20097877
'Liposomes are vesicular structures consisting of an aqueous core surrounded by a lipid bilayer. Apart from the cytosol and lysosomes, no other intracellular compartment has been successfully targeted using liposomal delivery. Here, we report the development of liposomes capable of specific targeting to the endoplasmic reticulum (ER) and associated membranes.
Role of lysine residues of plasma lipoproteins in high affinity binding to cell surface receptors on human fibroblasts.
Journal:J Biol Chem
PubMed ID:214439
'The low density lipoprotein (LDL) cell surface receptors on human fibroblasts grown in culture bind specific plasma lipoproteins, initiating a series of events which regulate intracellular cholesterol metabolism. Specificity for the interaction with the receptors resides with the protein moieties of the lipoproteins, specifically with the B and E apoproteins