Novex™ TBE-Urea Sample Buffer (2X)

For optimum performance, Novex™ TBE-Urea Sample Buffer is recommended for use with Novex™ TBE-urea gels. This buffer contains urea andRead more

Catalog Number	Quantity
LC6876	10 mL

Catalog number LC6876

Price (MXN)

Quantity:

10 mL

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For optimum performance, Novex™ TBE-Urea Sample Buffer is recommended for use with Novex™ TBE-urea gels. This buffer contains urea and the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, plus the tracking dyes bromophenol blue and xylene cyanol.

About Novex™ TBE-urea gels
Denaturing polyacrylamide TBE-urea gels resolve single-stranded DNA oligos or RNA into sharp, distinct bands. Novex™ TBE-urea gels are optimized for the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase protection assays (RPAs), in vitro transcription studies, and Northern blot analysis. Novex™ TBE-urea gels are designed to run on the XCell SureLock™ Mini-Cell.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

BufferSample Loading Buffer

Concentration2 X

For Use With (Application)Nucleic Acid Gel Electrophoresis, Blotting

Gel CompatibilityPolyacrylamide Gels, Agarose Gels

Label or DyeBromophenol Blue, Xylene Cyanol

Product LineNovex

Product TypeTBE Sample Buffer

Quantity10 mL

Shipping ConditionWet Ice

Unit SizeEach

Contents & Storage

Store at 2°C to 8°C

Frequently asked questions (FAQs)

My Novex TBE buffer has precipitated out of solution. What can I do?

If a slight turbidity develops, the fine precipitate can be dissolved by autoclaving for 5 minutes at 110°C. Do not autoclave in the container supplied. This treatment has no deleterious effect on the buffering properties of TBE.

Can a sample buffer with formamide be used with the Invitrogen TBE-Urea system?

There are many sample buffer formulations used, however we have found a distinct difference in the band appearance depending on the sample buffer composition. After evaluating urea, formamide, and various buffer systems, we found that the sharpest, flattest bands were obtained with a urea, Ficoll, and TBE buffer solution. Sample buffers made with formamide resulted in fuzzy, indistinct bands.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What can be used to stain Invitrogen precast TBE-Urea or TBE gels?

(1) Ethidium bromide: Soak gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 min. Destain by rinsing with three successive 10-min rinses of ultrapure water. Visualize bands under UV light. The gel must be removed from the cassette prior to visualization of the DNA under a UV light. Because polyacrylamide quenches the fluorescence of ethidium bromide, it is not possible to detect bands that contain less than about 10 ng of DNA by this method. SilverXpress and SYBR stains will provide greater detection sensitivity.

(2) Invitrogen SilverXpress Stain: Follow the standard procedure from the instruction booklet for staining TBE or TBE-Urea gels.

(3) SYBR Green I/II Nucleic Acid Gel Stain: See the SYBR Green Staining Manual for protocol details.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

(1) Ethidium bromide: Soak gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 min. Destain by rinsing with three successive 10-min rinses of ultrapure water. Visualize bands under UV light. The gel must be removed from the cassette prior to visualization of the DNA under a UV light. Because polyacrylamide quenches the fluorescence of ethidium bromide, it is not possible to detect bands that contain less than about 10 ng of DNA by this method. SilverXpress and SYBR stains will provide greater detection sensitivity.

(2) SilverXpress Stain: Follow the standard procedure from the instruction booklet for staining TBE or TBE-Urea gels.

(3) SYBR Green I Nucleic Acid Gel Stain (Cat. No. S7585)/SYBR Green II RNA Gel Stain (Cat. No. S7564): See the SYBR Green Staining Manual for protocol details.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.