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Citations & References (9)
Invitrogen™
Qdot™ 565 ITK™ Amino (PEG) Quantum Dots
Qdot™ 565 ITK™ amino (PEG) quantum dots are the ideal starting material for preparing custom conjugates of ultrabright and photostableRead more
| Catalog Number | Quantity |
|---|---|
| Q21531MP | 250 μL |
Catalog number Q21531MP
Price (MXN)
-
Quantity:
250 μL
Qdot™ 565 ITK™ amino (PEG) quantum dots are the ideal starting material for preparing custom conjugates of ultrabright and photostable fluorescently labeled proteins or other biopolymers. These probes are functionalized with amine-derivatized PEG, which prevents non-specific interactions and provides a convenient handle for conjugation. The amino quantum dots react efficiently with isothiocyanates and succinimidyl esters, or with native carboxylic acids using water-soluble carbodiimides such as EDC. Such derivatives may be used for various labeling and tracking applications that require ultrabright and stable fluorescence. Our Qdot™ ITK™ amino quantum dots are provided as 8 μM solutions and are available in 8 colors of Qdot™ probes.
Important Features of Qdot™ ITK™ Amino Quantum Dots:
• Qdot™ 565 ITK™ amino quantum dot has emission maxima of ∼565 nm
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large stokes shift
• Available in multiple colors
• Ideal for various labeling and tracking applications
Properties of Qdot™ Nanocrystals
Qdot™ probes are ideal for imaging and labeling applications that require bright fluorescent signals and/or real-time tracking. Unique among fluorescent reagents, all nine available colors of Qdot™ probes can be simultaneously excited with a single (UV to blue-green) light source. This property makes these reagents excellent for economical and user-friendly multiplexing applications. Qdot™ labels are based on semiconductor nanotechnology and are similar in scale to moderately sized proteins.
About the Innovator’s Tool Kit Qdot™ ITK™ Reagents
These Qdot™ ITK™ probes are ideal for researchers who wish to prepare specific (non-stocked) conjugates for their applications and need customizable conjugation functionality.
Other Forms of Qdot™ Nanocrystals are Available
In addition to the amine-derivatized form, we offer Qdot™ ITK™ quantum dots with carboxyl and aliphatic hydrocarbon modifications. We’ve also developed a wide range of Qdot™ nanocrystals conjugates and labeling kits. Investigate the properties of Qdot™ nanocrystals or read the Molecular Probes™ Handbook Section 6.6—Qdot™ Nanocrystals to find out more.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Important Features of Qdot™ ITK™ Amino Quantum Dots:
• Qdot™ 565 ITK™ amino quantum dot has emission maxima of ∼565 nm
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large stokes shift
• Available in multiple colors
• Ideal for various labeling and tracking applications
Properties of Qdot™ Nanocrystals
Qdot™ probes are ideal for imaging and labeling applications that require bright fluorescent signals and/or real-time tracking. Unique among fluorescent reagents, all nine available colors of Qdot™ probes can be simultaneously excited with a single (UV to blue-green) light source. This property makes these reagents excellent for economical and user-friendly multiplexing applications. Qdot™ labels are based on semiconductor nanotechnology and are similar in scale to moderately sized proteins.
About the Innovator’s Tool Kit Qdot™ ITK™ Reagents
These Qdot™ ITK™ probes are ideal for researchers who wish to prepare specific (non-stocked) conjugates for their applications and need customizable conjugation functionality.
Other Forms of Qdot™ Nanocrystals are Available
In addition to the amine-derivatized form, we offer Qdot™ ITK™ quantum dots with carboxyl and aliphatic hydrocarbon modifications. We’ve also developed a wide range of Qdot™ nanocrystals conjugates and labeling kits. Investigate the properties of Qdot™ nanocrystals or read the Molecular Probes™ Handbook Section 6.6—Qdot™ Nanocrystals to find out more.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityCarboxylic Acid, Ketone, Aldehyde
Concentration8 μM
Emission565
Label or DyeQdot™ 565
Product TypeQuantum Dot
Quantity250 μL
Reactive MoietyAmine, Primary Amine
Shipping ConditionRoom Temperature
Label TypeQdot Nanocrystals
Product LineITK, Qdot
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C).
Frequently asked questions (FAQs)
How large are the Qdot nanocrystals?
What is the best way to remove white precipitate from my ITK Qdot nanocrystals?
I see a white precipitate in my ITK Qdot nanocrystals; should I be concerned?
Why do my Qdot nanocrystals appear to be blinking?
My Qdot nanocrystals were brightly fluorescent before I mounted my samples; now I'm seeing a loss of fluorescence. Why is this happening?
Citations & References (9)
Citations & References
Abstract
Development of homogeneous binding assays based on fluorescence resonance energy transfer between quantum dots and Alexa Fluor fluorophores.
Journal:Anal Biochem
PubMed ID:16860286
'We studied the fluorescence resonance energy transfer (FRET) between quantum dots emitting at 565, 605, and 655 nm as energy donors and Alexa Fluor fluorophores with absorbance maxima at 594, 633, 647, and 680 nm as energy acceptors. As a first step, we prepared covalent conjugates between all three types
Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.
Journal:ACS Nano
PubMed ID:23181687
'We present a methodology for targeting quantum dots to specific proteins on living cells in two steps. In the first step, Escherichia coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a 13 amino acid recognition sequence that is genetically fused to a protein of interest. In the second
Imaging a single quantum dot when it is dark.
Journal:Nano Lett
PubMed ID:18671437
'We have succeeded in recording extinction images of individual cadmium selenide quantum dots at ambient condition. This is achieved by optimizing the interference between the light that is coherently scattered from the quantum dot and the reflection of the incident laser beam. The ability to interrogate the dot in the
Real-time imaging of astrocyte response to quantum dots: in vivo screening model system for biocompatibility of nanoparticles.
Journal:Nano Lett
PubMed ID:17638392
Astrocytes are the principle macroglial brain cells. They are activated by different stressors and brain injuries. Quantum dots (QDs) can cause oxidative stress. This study shows a real-time imaging of primary cortical cultures and assessment of QD-induced activation of astrocytes in the brains of transgenic mice with the luciferase gene
Variables influencing interactions of untargeted quantum dot nanoparticles with skin cells and identification of biochemical modulators.
Journal:Nano Lett
PubMed ID:17408303
Skin cells (NHEK) take up untargeted quantum dots (QD) with surface polyethylene glycol (PEG), amines, and carboxylic acids, but the mechanisms are unknown. Time courses of QD-NHEK interactions were determined and effects of QD surface coating, temperature, culture medium supplements and inhibitors of the cell cycle and endocytosis identified. The