SYTO™ Blue Fluorescent Nucleic Acid Stain Sampler Kit (SYTO™ dyes 40, 41, 42, 45)
SYTO™ Blue Fluorescent Nucleic Acid Stain Sampler Kit (SYTO™ dyes 40, 41, 42, 45)
Citations & References (2)
Invitrogen™

SYTO™ Blue Fluorescent Nucleic Acid Stain Sampler Kit (SYTO™ dyes 40, 41, 42, 45)

The SYTO Blue Fluorescent Nucleic Acid Stain Sampler Kit contains a collection of cell-permeant, blue-fluorescent SYTO nucleic acid stains (SYTORead more
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Catalog NumberQuantity
S113501 kit
Catalog number S11350
Price (MXN)
-
Quantity:
1 kit

The SYTO Blue Fluorescent Nucleic Acid Stain Sampler Kit contains a collection of cell-permeant, blue-fluorescent SYTO nucleic acid stains (SYTO dyes 40, 41, 42, 45). Because the dyes may demonstrate different staining behaviors with various tissues and cells, it may be necessary to test the dyes to find the optimal dye for a specific application. The kit contains 50 μL of each SYTO dye.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorBlue
Detection MethodFluorescence
Dye TypeCell-Permeant
Excitation Wavelength Rangevarious
For Use With (Equipment)Fluorescence Microscope
Product LineSYTO
Quantity1 kit
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeNucleic Acid Stain
SubCellular LocalizationNucleic Acids
Unit SizeEach
Contents & Storage
The kit contains 50 μL of each SYTO dye.
  • Store in freezer -5°C to -30°C and protect from light.

    • Frequently asked questions (FAQs)

    How do SYTO dyes bind to DNA?

    The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

    1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
    2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
    3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
    5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

    SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Citations & References (2)

    Citations & References
    Abstract
    Assessment of fluorochromes for two-photon laser scanning microscopy of biofilms.
    Authors:Neu TR, Kuhlicke U, Lawrence JR
    Journal:Appl Environ Microbiol
    PubMed ID:11823234
    A major limitation for the use of two-proton laser scanning microscopy (2P-LSM) in biofilm and other studies is the lack of a thorough understanding of the excitation-emission responses of potential fluorochromes. In order to use 2P-LSM, the utility of various fluorochromes and probes specific for a range of biofilm constituents ... More
    Novel model for multispecies biofilms that uses rigid gas-permeable lenses.
    Authors:Peyyala R, Kirakodu SS, Ebersole JL, Novak KF,
    Journal:Appl Environ Microbiol
    PubMed ID:21421785
    Oral biofilms comprise complex multispecies consortia aided by specific inter- and intraspecies interactions occurring among commensals and pathogenic bacterial species. Oral biofilms are primary initiating factors of periodontal disease, although complex multifactorial biological influences, including host cell responses, contribute to the individual outcome of the disease. To provide a system ... More