SYTO™ 82 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

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SYTO 82 dye tends to stain RNA as well as DNA. Better options for cell permeant nucleic acid stains for flow cytometry analysis of DNA content are the cell cycle stains such as:
- Vybrant DyeCycle Violet Stain (Cat. No. V35003)
- Vybrant DyeCycle Green Stain (Cat. No. V35004)
- Vybrant DyeCycle Orange Stain (Cat. No. V35005)
- Vybrant DyeCycle Ruby Stain (Cat. No. V10273)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.