Staphylococcus aureus (Wood strain without protein A) BioParticles™, unlabeled

The Molecular Probes™ unlabeled BioParticles™ product line comprises heat- or chemically killed S. aureus (Wood strain, without protein A) andRead more

Catalog Number	Quantity
S2859	100 mg

Catalog number S2859

Price (MXN)

Quantity:

100 mg

The Molecular Probes™ unlabeled BioParticles™ product line comprises heat- or chemically killed S. aureus (Wood strain, without protein A) and zymosan (S. cerevisiae) cells. These nonfluorescent BioParticles™ products are useful in phagocytosis assays, both as controls and for custom-labeling with reactive dyes.

BioParticles Specifications:
• Label (Ex/Em): Unlabeled
• Particle: S. aureus (Wood strain, without protein A)
• Opsonizing reagent available

Using BioParticles™ Products
BioParticles™ conjugates are provided as lyophilized powders. There are approximately 3 x 108 S. aureus particles per mg solid and approximately 2 x 107 zymosan particles per mg solid. BioParticles™ conjugates can be reconstituted in the buffer of your choice for use in phagocytosis assays.

Find More BioParticles™ Products
We offer a large range of dye-labeled and unlabeled E. coli (K-12 strain), S. aureus (Wood strain, without protein A), and zymosan (S. cerevisiae) BioParticles™ products. Find out about these products and their applications by reviewing Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes™ Handbook.

For pH-sensitive endocytosis assays, see our fluorescent pHrodo™ BioParticles™ conjugates.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Dye TypeUnconjugated

FormLyophilized Powder

Quantity100 mg

Shipping ConditionRoom Temperature

SpeciesS. aureus

For Use With (Equipment)Fluorescence Microscope

Product LineBioParticles

Product TypeBioparticle Unlabeled

Unit SizeEach

Contents & Storage

Store in freezer (-5 to -30°C).

Frequently asked questions (FAQs)

Are the Invitrogen BioParticles products sterile?

While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.

What is the concentration of bacterial particles for the Staphylococcus aureus (Wood strain without protein A) BioParticles, unlabeled (Cat. No. S2859)?

Staphylococcus aureus (Wood strain without protein A) BioParticles, unlabeled (Cat. No. S2859) contains 3 x 10⁸particles/mg.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the type of bond that attaches the dyes to the BioParticles probes?

We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What can the BioParticles product line be used for?

Fluorescent Bioparticles have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E.Coli, S. aureus, and zymosan BioParticles conjugates covalently labeled with a variety of different fluorophores.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (1)

Citations & References

Abstract

Dectin-2 is a pattern recognition receptor for fungi that couples with the Fc receptor gamma chain to induce innate immune responses.

Authors:Sato K, Yang XL, Yudate T, Chung JS, Wu J, Luby-Phelps K, Kimberly RP, Underhill D, Cruz PD, Ariizumi K

Journal:J Biol Chem

PubMed ID:17050534

'Antigen presenting cells recognize pathogens via pattern recognition receptors (PRR), which upon ligation transduce intracellular signals that can induce innate immune responses. Because some C-type lectin-like receptors (e.g. dectin-1 and DCSIGN) were shown to act as PRR for particular microbes, we considered a similar role for dectin-2. Binding assays using ... More