TransFluoSpheres™ Carboxylate-Modified Microspheres, 1.0 μm (488/645), 2% solids
TransFluoSpheres™ Carboxylate-Modified Microspheres, 1.0 μm (488/645), 2% solids
Citations & References (7)
Invitrogen™

TransFluoSpheres™ Carboxylate-Modified Microspheres, 1.0 μm (488/645), 2% solids

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed fromRead more
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Catalog NumberQuantity
T88830.5 mL
Catalog number T8883
Price (MXN)
-
Quantity:
0.5 mL
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes™ TransFluoSpheres™ beads are manufactured using high-quality, ultraclean polystyrene and are loaded with two (or more) fluorescent dyes to produce a microspheres that can be excited by the 488 nm or the 514 nm spectral lines of the argon-ion laser but emit at much longer wavelengths, facilitating multicolor experiments and allowing the use of broadband excitation and emission filters. TransFluoSpheres™ microspheres typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.

TransFluoSpheres™ Microsphere Specifications:

• Label (Ex/Em): Crimson-fluorescent (488/645)
• Nominal bead diameter: 1.0 μm
• Coupling surface: Carboxylate
• Solids: 2%

Characteristics of the Carboxylate Coupling Surface
Carboxylate-modified FluoSpheres™ beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.

Key Applications of Microspheres:
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 μm, 0.04 μm, 0.1 μm, 0.2 μm, 0.5 μm, 1.0 μm, 2.0 μm, 4.0 μm, 10.0 μm, and 15.0 μm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product LineTransFluoSpheres
Quantity0.5 mL
Shipping ConditionRoom Temperature
Surface ModificationCarboxylate
ColorCrimson
Diameter (Metric)1 μm
MaterialPolystyrene
Product TypeCarboxylate-Modified Microsphere
Unit Size0.5 mL
Contents & Storage
Store in refrigerator (2–8°C) and protect from light.

Frequently asked questions (FAQs)

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Bacterial contamination is the most common cause of microspheres becoming unusable. Many of our particles are supplied with a low level of sodium azide to prevent bacterial contamination, but sometimes this can still occur. Bacterial contamination is best assessed by plating on appropriate growth medium and checking the plates after 72 hr.

Find additional tips, troubleshooting help, and resources within ourMicrospheres Support Center.

I accidentally froze my microspheres; can I still use them?

Even brief freezing can cause irreversible aggregation and potential distortion of the bead shape. You should not use these microspheres.

Find additional tips, troubleshooting help, and resources within our Microspheres Support Center.

My protein-coated microspheres appear to be non-specifically binding in my experimental system. Do you have a product that can help reduce these non-specific interactions?

Non-specific binding can often be relieved by a blocking solution, but microspheres seem to require a stronger blocking solution than those most commonly commercially available. Hence, we've developed the BlockAid Blocking Solution (Cat. No. B10710). This reagent is a protein-based blocking solution designed for use with FluoSpheres microspheres and TransFluoSpheres microspheres conjugated to biotin, streptavidin, NeutrAvidin biotin-binding protein, or other proteins. The BlockAid Blocking Solution has proven useful for reducing the nonspecific binding of protein-coated or other macromolecule-coated microspheres in a wide variety of flow cytometry, microscopy, and microarray applications.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all TransFluoSpheres™ Carboxylate-Modified Microspheres, 1.0 μm (488/645), 2% solids FAQs

Citations & References (7)

Citations & References
Abstract
A single amino acid in the cytoplasmic domain of the beta 2 integrin lymphocyte function-associated antigen-1 regulates avidity-dependent inside-out signaling.
Authors:Bleijs DA, van Duijnhoven GC, van Vliet SJ, Thijssen JP, Figdor CG, van Kooyk Y
Journal:J Biol Chem
PubMed ID:11134023
'The leukocyte-specific beta(2) integrin lymphocyte function-associated antigen-1 (LFA-1) (alpha(L)/beta(2)) mediates activation-dependent adhesion to intercellular adhesion molecule (ICAM)-1. In leukocytes, LFA-1 requires activation by intracellular messengers to bind ICAM-1. We observed malfunctioning of LFA-1 activation in leukemic T cells and K562-transfected cells. This defective inside-out integrin activation is only restricted to ... More
DC-SIGN-ICAM-2 interaction mediates dendritic cell trafficking.
Authors:Geijtenbeek TB, Krooshoop DJ, Bleijs DA, van Vliet SJ, van Duijnhoven GC, Grabovsky V, Alon R, Figdor CG, van Kooyk Y
Journal:Nat Immunol
PubMed ID:11017109
'Dendritic cells (DCs) are recruited from blood into tissues to patrol for foreign antigens. After antigen uptake and processing, DCs migrate to the secondary lymphoid organs to initiate immune responses. We now show that DC-SIGN, a DC-specific C-type lectin, supports tethering and rolling of DC-SIGN-positive cells on the vascular ligand ... More
Comparison of methods for monitoring bacterial transport in the subsurface.
Authors:DeFlaun MF, Fuller ME, Zhang P, Johnson WP, Mailloux BJ, Holben WE, Kovacik WP, Balkwill DL, Onstott TC
Journal:J Microbiol Methods
PubMed ID:11576686
'The purpose of this study was to compare in a laboratory experiment, a suite of methods developed to track viable bacteria during field transport experiments. The criteria for development and selection of these methods included: (1) the ability to track bacteria within the environment from which they were isolated; (2) ... More
Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses.
Authors:Geijtenbeek TB, Torensma R, van Vliet SJ, van Duijnhoven GC, Adema GJ, van Kooyk Y, Figdor CG
Journal:Cell
PubMed ID:10721994
Contact between dendritic cells (DC) and resting T cells is essential to initiate a primary immune response. Here, we demonstrate that ICAM-3 expressed by resting T cells is important in this first contact with DC. We discovered that instead of the common ICAM-3 receptors LFA-1 and alphaDbeta2, a novel DC-specific ... More
DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells.
Authors:Geijtenbeek TB, Kwon DS, Torensma R, van Vliet SJ, van Duijnhoven GC, Middel J, Cornelissen IL, Nottet HS, KewalRamani VN, Littman DR, Figdor CG, van Kooyk Y
Journal:Cell
PubMed ID:10721995
Dendritic cells (DC) capture microorganisms that enter peripheral mucosal tissues and then migrate to secondary lymphoid organs, where they present these in antigenic form to resting T cells and thus initiate adaptive immune responses. Here, we describe the properties of a DC-specific C-type lectin, DC-SIGN, that is highly expressed on ... More
View all TransFluoSpheres™ Carboxylate-Modified Microspheres, 1.0 μm (488/645), 2% solids citations