Embryonic stem-cell FBS, qualified, USDA-approved regions

Name: Embryonic stem-cell FBS, qualified, USDA-approved regions
SKU: 10439001

Los sueros bovinos fetales Gibco™ ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayosMás información

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Número de catálogo	Cantidad	Formato
10439001	50 mL	One Shot
10439024	500 mL	Frasco

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Número de catálogo 10439001

Precio (MXN)

Cantidad:

50 mL

Formato:

One Shot

Los sueros bovinos fetales Gibco™ ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayos específicos, ganando la confianza de los investigadores con una calidad uniforme y un soporte galardonado que ayuda a satisfacer sus necesidades de investigación y presupuesto

Categoría de los sueros: Especialidad
Origen: Países que cumplen los requisitos de importación del USDA.
Se han sometido a pruebas específicas para comprobar la capacidad de mantener la morfología celular no diferenciada de las células madre embrionarias.
Nivel de endotoxinas: ≤50 EU/ml (niveles habituales ≤10 EU/ml).
Nivel de hemoglobina: ≤25 mg/dl.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.

Especificaciones

Grupo de donantesNo

Concentración de endotoxinas<50 EU/mL

Concentración de hemoglobina≤25 mg/dL

Línea de productosOne Shot

Grado de pureza o calidadES Cell-Qualified

Condiciones de envíoFrozen

EspecieCattle/Bovine

Probado paraBovine Respiratory Syncytial Virus FA, Cytopathic Agents, Hemadsorbing Agents, Reovirus FA, Rabies FA, Hemoglobin (ng/dL), Blue Tongue Virus, Bovine Adenovirus FA, Bovine Parvovirus FA, Osmolality, Sp2 Performance Assay, Electrophoretic Pattern, Total Protein, Endotoxin (EU/mL), Mycoplasma, Sterility Testing (Bacteria, Fungi)

AgeFetal

País de origenMéxico

FormularioLíquido

FormatoOne Shot

Tipo de productoFetal Bovine Serum

Cantidad50 mL

Serum TreatmentStandard (Sterile-filtered)

EsterilidadEstéril

Unit SizeEach

Contenido y almacenamiento

Condiciones de almacenamiento: ≤10 °C
Condiciones de envío: Congelado

Preguntas frecuentes

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Do I need to heat-inactivate Embryonic Stem-Cell FBS, qualified?

The material is not heat-inactivated. We would only recommend heat-inactivating it if your application specifically requires it. We recommend using heat-inactivated serum when working with immune type cells or for immunological applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.