Plásmido pCMV·SPORT-βgal

El plásmido pCMV·SPORT-bgal se utiliza como control positivo parasupervisar la expresión en células eucariotas. El plásmido contiene el gen indicadorb-galactosidasaMás información

Número de catálogo	Cantidad
10586014	25μg

Número de catálogo 10586014

Precio (MXN)

Cantidad:

El plásmido pCMV·SPORT-bgal se utiliza como control positivo para
supervisar la expresión en células eucariotas. El plásmido contiene el gen indicador
b-galactosidasa (b-gal) de E. coli clonado como fragmento no I
en el plásmido pCMV·SPORT1. El plásmido también contiene
el promotor de CMV por encima del gen b-gal, seguido por el t-intron SV40
y la señal de poliadenilación. El gen b-lactamasa permite seleccionar
la resistencia a la ampicilina en E. coli.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Sistema constitutivo o inducibleConstitutivo

Tipo de entregaTransfección

Para utilizar con (aplicación)Ensayos con indicadores, expresión constitutiva

Tipo de productoPlásmido

Cantidad25μg

Gen marcadorBeta-gal (lacZ)

Agente de selección (eucariótico)Ninguno

Condiciones de envíoAprobado para su envío en hielo húmedo o seco

VectorpCMV

PromotorCMV

Etiqueta de proteínaSin etiquetar

Unit SizeEach

Contenido y almacenamiento

Almacenar a +4 °C. Almacenar a -20 °C a largo plazo.

Preguntas frecuentes

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I sequenced one of your vectors after PCR amplification and observed a difference from what is provided online (or in the manual). Should I be concerned?

Our vectors have not been completely sequenced. Your sequence data may differ when compared to what is provided. Known mutations that do not affect the function of the vector are annotated in public databases.

Are your vectors routinely sequenced?

No, our vectors are not routinely sequenced. Quality control and release criteria utilize other methods.

How was the reference sequence for your vectors created?

Sequences provided for our vectors have been compiled from information in sequence databases, published sequences, and other sources.

Citations & References (1)

Citations & References

Abstract

TNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2.

Authors: Li Xiaoming; Yang Yili; Ashwell Jonathan D;

Journal:Nature

PubMed ID:11907583

Tumour necrosis factor-alpha (TNF-alpha) is a proinflammatory mediator that exerts its biological functions by binding two TNF receptors (TNF-RI and TNF-RII), which initiate biological responses by interacting with adaptor and signalling proteins. Among the signalling components that associate with TNF receptors are members of the TNF-R-associated factor (TRAF) family. TRAF2 ... More