Higromicina B (50 mg/ml)
Higromicina B (50 mg/ml)
Citas y referencias (38)
Gibco™

Higromicina B (50 mg/ml)

La higromicina B es un antibiótico soluble en agua purificado a partir de la bacteria Streptomyces kanamyceticus. La higromicina BMás información
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Número de catálogoCantidad
1068701020 mL
Número de catálogo 10687010
Precio (MXN)
-
Cantidad:
20 mL
La higromicina B es un antibiótico soluble en agua purificado a partir de la bacteria Streptomyces kanamyceticus. La higromicina B Gibco™ se utiliza como antibiótico de selección bacteriana en el intervalo de concentraciones de 200 a 500 μg/ml. Este producto se suministra como una solución de 50 mg/ml.

La resistencia a la higromicina B se obtiene por el gen de resistencia a la higromicina de E. coli (hyg o hph). La higromicina B tiene un modo de acción diferente a los reactivos de selección Geneticin™, blasticidina S o Zeocin™, lo que hace que sea una opción excelente para los experimentos de selección doble (consulte la selección de antibióticos).

Otras opciones y más información
Ofrecemos una amplia gama de antibióticos y antimicóticos en formatos tanto de polvo como líquidos.

Consulte la lista completao busque productos para:
• Control de la contaminación
• Selección de bacterias y eucariotas

Consulte las recomendaciones para conocer las concentraciones de trabajo para seleccionar los antibióticos.

Obtenga más información sobre el uso de antibióticos y antimicóticos en cultivo celular y revise las directrices para la descontaminación de cultivos.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración50 mg/mL
Tipo de cultivoCultivo celular de mamíferos, cultivo celular de insectos
Para utilizar con (aplicación)Selección⁄generación de líneas celulares estables eucariotas
Cantidad20 mL
Condiciones de envíoTemperatura ambiente
FormularioLíquido
Tipo de productoHigromicina
EsterilidadEstéril
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 2 a 8 °C
Condiciones de envío: Ambiente

Preguntas frecuentes

Which of your antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in mammalian cells?

All of our antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for making multiple stable cell lines. However, kill curves will need to be performed for each combination of antibiotics since sensitivity to a given antibiotic tends to increase when combined with other antibiotics.

What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

What is the range of specific activity of Hygromycin B?

The specific activity range of Hygromycin B is from 386-482 micrograms/mg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What solvent is used to prepare your Hygromycin B solution? How should it be stored?

Hygromycin B is supplied as a 50 mg/mL solution in phosphate buffered saline. It is manufactured aseptically and sterile filtered, and is suitable for use in tissue culture applications. We recommend storing it at 2-8 degrees C.

What is the mode of action on the following antibiotics: Blasticidin, Geneticin (G418), Hygromycin, and Zeocin?

Blasticidin: Nucleoside Inhibits protein synthesis in prokaryotic and eukaryotic cells by interfering with peptidyl transfer reaction of protein synthesis, causing early termination of translation.

Geneticin (G418): Aminoglycoside Blocks protein synthesis in mammalian cells by interfering with ribosomal function.

Hygromycin: Aminocyclitol Inhibits protein synthesis by disrupting translocation and promoting mistranslation.

Zeocin: Intercalates with DNA and cleaves it.

View all Higromicina B (50 mg/ml) FAQs

Citations & References (38)

Citations & References
Abstract
WAF1, a potential mediator of p53 tumor suppression.
Authors:el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW, Vogelstein B
Journal:Cell
PubMed ID:8242752
The ability of p53 to activate transcription from specific sequences suggests that genes induced by p53 may mediate its biological role as a tumor suppressor. Using a subtractive hybridization approach, we identified a gene, named WAF1, whose induction was associated with wild- type but not mutant p53 gene expression in ... More
CC chemokine receptor 5-mediated signaling and HIV-1 Co-receptor activity share common structural determinants. Critical residues in the third extracellular loop support HIV-1 fusion.
Authors:Alkhatib G, Ahuja SS, Light D, Mummidi S, Berger EA, Ahuja SK
Journal:J Biol Chem
PubMed ID:9242636
'There is a close correspondence between the ability of RANTES and macrophage inflammatory proteins 1alpha and 1beta to activate CC chemokine receptor 5 (CCR5) and the ability to inhibit CCR5-dependent membrane fusion mediated by the envelope glycoprotein of human immunodeficiency virus (HIV), type 1. This finding suggests that some of ... More
Dimerization of a selectin and its ligand stabilizes cell rolling and enhances tether strength in shear flow.
Authors: Ramachandran V; Yago T; Epperson T K; Kobzdej M M; Nollert M U; Cummings R D; Zhu C; McEver R P;
Journal:Proc Natl Acad Sci U S A
PubMed ID:11526223
'Selectins mediate rolling of leukocytes by rapid formation and dissociation of selectin-ligand bonds, which are assumed to require high mechanical strength to prevent premature dissociation by the forces applied in shear flow. This assumption is based largely on the observation that increasing wall shear stress increases only modestly the dissociation ... More
Reconstitution of stretch-activated cation channels by expression of the alpha-subunit of the epithelial sodium channel cloned from osteoblasts [published erratum appears in Proc Natl Acad Sci U S A 1997 Apr 15;94(8):4233]
Authors:Kizer N, Guo XL, Hruska K
Journal:Proc Natl Acad Sci U S A
PubMed ID:9023374
'Osteoblasts respond to repetitive strain by activating stretch- activated, nonselective cation channels (SA-CAT) and increasing matrix protein production. SA-CAT channels are thought to be responsible for mechano-transduction in osteoblasts, although the molecular identity of the SA-CAT channel has previously been unknown. We have demonstrated that both the UMR-106 osteoblast-like cell ... More
Genetic analysis of the herpes simplex virus type 1 UL20 protein domains involved in cytoplasmic virion envelopment and virus-induced cell fusion.
Authors:Melancon JM, Foster TP, Kousoulas KG,
Journal:J Virol
PubMed ID:15220406
'The herpes simplex virus type 1 UL20 protein (UL20p) is an important determinant for cytoplasmic virion morphogenesis and virus-induced cell fusion. To delineate the functional domains of the UL20 protein, we generated a panel of single and multiple (cluster) alanine substitutions as well as UL20p carboxyl-terminal truncations. The UL20 mutant ... More
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