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Citas y referencias (2)
Invitrogen™

Platinum™ Taq DNA Polymerase, Brasil

Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
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Número de catálogoN.º de reacciones
10966030500 reacciones
10966020100 reacciones
109661745000 reacciones
1096618225,000 Reactions
Número de catálogo 10966030
Precio (MXN)
3,188.46
Each
N.º de reacciones:
500 reacciones
Precio (MXN)
3,188.46
Each
Platinum Taq DNA Polymerase is a convenient and reliable hot start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The hot start property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step.

Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template–dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity (but not 3' to 5' exonuclease activity). PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications.

Features

  • Assembly of PCR reactions at room temperature
  • Hot-start kinetics reduce nonspecific primer annealing and improve product yield
  • May be used for PCR products up to 10 kb
  • Optional KB Extender provides more versatility in PCR assays

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Fidelidad (frente a Taq)1X
Inicio en calienteBuilt-In Hot Start
N.º de reacciones500 reacciones
Sobrante3'-A
PolimerasaPlatinum Taq DNA Polymerase
Cantidad500 reactions
Condiciones de envíoDry Ice
Tamaño (producto final)10 kb or less
Material de partidaADN
Concentración5 U/μL
Para utilizar con (aplicación)Hot-start PCR
GC-Rich PCR PerformanceMedio
Velocidad de reacciónEstándar
Unit SizeEach

Citations & References (2)

Citations & References
Abstract
Dynamin 2 mutations associated with human diseases impair clathrin-mediated receptor endocytosis.
Authors:Bitoun M, Durieux AC, Prudhon B, Bevilacqua JA, Herledan A, Sakanyan V, Urtizberea A, Cartier L, Romero NB, Guicheney P,
Journal:Hum Mutat
PubMed ID:19623537
Dynamin 2 (DNM2) is a large GTPase involved in the release of nascent vesicles during endocytosis and intracellular membrane trafficking. Distinct DNM2 mutations, affecting the middle domain (MD) and the Pleckstrin homology domain (PH), have been identified in autosomal dominant centronuclear myopathy (CNM) and in the intermediate and axonal forms ... More
Utility and accuracy of template-directed dye-terminator incorporation with fluorescence-polarization detection for genotyping single nucleotide polymorphisms.
Authors: Akula N; Chen Y S; Hennessy K; Schulze T G; Singh G; McMahon F J;
Journal:Biotechniques
PubMed ID:12019780
There are little independent data available about how well single nucleotide polymorphism (SNP) genotyping technologies perform in the typical molecular genetics laboratory. We evaluated the utility and accuracy of a widely used technology, template-directed dye-terminator incorporation with fluorescence-polarization detection (FP-TDI), in a sample of 177 SNPs selected solely on the ... More