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Citas y referencias (8)
Gibco™
MEM
MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be usedMás información
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| Número de catálogo | Cantidad |
|---|---|
| 11095080 | 500 mL |
| 11095098 | 10 × 500 mL |
| 11095072 | 1000 mL |
| 11095114 | 6 x 1000 mL |
Número de catálogo 11095080
Precio (MXN)
860.35
Each
Cantidad:
500 mL
Precio (MXN)
860.35
Each
MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
| With | Without |
| • L-glutamine | • HEPES |
| • Phenol Red |
The complete formulation is available.
Using MEM
MEM was developed by Harry Eagle, based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Para su uso en investigación o procesos de fabricación posteriores. No apto para uso diagnóstico ni para la administración directa en seres humanos ni en animales.
Especificaciones
Línea de célulasHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7 y fibroblastos
Tipo de célulaAstrocitos de rata primarios
Concentración1 X
Calidad de fabricacióncGMP-compliant under the ISO 13485 standard
Línea de productosGibco
Tipo de productoMEM (medio esencial mínimo)
Cantidad500 mL
Duración de almacenamiento12 meses a partir de la fecha de fabricación
Condiciones de envíoTemperatura ambiente
ClasificaciónLibre de material de origen animal
FormularioLíquido
Serum LevelSuplementos de suero estándar
EsterilidadEstéril con filtro
Sterilization MethodEstéril con filtro
Con aditivosBajo nivel de glucosa, Glutamina, Rojo de fenol
Sin aditivosSin HEPES, Sin piruvato sódico
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 2 °C a 8 °C (proteger de la luz)
Condiciones de envío: Ambiente
Vida útil: 12 meses a partir de la fecha de fabricación
Condiciones de envío: Ambiente
Vida útil: 12 meses a partir de la fecha de fabricación
Preguntas frecuentes
Where can I find the osmolality for MEM Medium?
What is the shelf life of the DMEM, high glucose, pyruvate medium once the bottle is opened and the medium is supplemented?
Is it necessary to store DMEM, high glucose, pyruvate medium in the dark?
How long can I keep my media after supplementing with serum?
My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
Citations & References (8)
Citations & References
Abstract
Influx of calcium through a redox-sensitive plasma membrane channel in thymocytes causes early necrotic cell death induced by the epipolythiodioxopiperazine toxins.
Journal:J Biol Chem
PubMed ID:12063251
'Gliotoxin, a member of the epipolythiodioxopiperazine (ETP) class of toxins, induces both apoptotic and necrotic cell death in a concentration-dependent manner. Whereas the specific trigger for apoptotic death caused by these toxins is unclear, the reactive disulfide bond in the ETP toxins is required for biological activity. Thus it is
Antagonism between Ena/VASP Proteins and Actin Filament Capping Regulates Fibroblast Motility.
Journal:Cell
PubMed ID:12086607
'Cell motility requires lamellipodial protrusion, a process driven by actin polymerization. Ena/VASP proteins accumulate in protruding lamellipodia and promote the rapid actin-driven motility of the pathogen Listeria. In contrast, Ena/VASP negatively regulate cell translocation. To resolve this paradox, we analyzed the function of Ena/VASP during lamellipodial protrusion. Ena/VASP-deficient lamellipodia protruded
A serum- and antioxidant-free primary culture model of mouse cortical neurons for pharmacological screen and studies of neurotrophic and neuroprotective agents.
Journal:Cell Mol Neurobiol
PubMed ID:12363202
'1. Morphologically developmental properties of fetal mouse cortical neurons in the chemically defined serum- and antioxidant-free culture condition were observed. Also, cellular composition in cultures was identified by immunostaining with anti-NSE and anti-GFAP. 2. Various cell densities ranging from 1 x 10(3) to 1 x 10(6) cells/cm2 were prepared to
Acute hippocampal slice preparation and hippocampal slice cultures.
Journal:Methods Mol Biol
PubMed ID:21815062
'A major advantage of hippocampal slice preparations is that the cytoarchitecture and synaptic circuits of the hippocampus are largely retained. In neurotoxicology research, organotypic hippocampal slices have mostly been used as acute ex vivo preparations for investigating the effects of neurotoxic chemicals on synaptic function. More recently, hippocampal slice cultures,
The Discodermia calyx toxin calyculin a enhances cyclin D1 phosphorylation and degradation, and arrests cell cycle progression in human breast cancer cells.
Journal:Toxins (Basel)
PubMed ID:22069692
'Cyclin D1 is a key regulator of the cell cycle that is over expressed in more than half of breast cancer patients. The levels of cyclin D1 are controlled primarily through post-translational mechanisms and phosphorylation of cyclin D1 at T286 induces its proteasomal degradation. To date, no studies have explored