Medio 199, sales de Earle
Medio 199, sales de Earle
Citas y referencias (5)
Gibco™

Medio 199, sales de Earle

El medio 199 se desarrolló originalmente para estudios nutricionales de fibroblastos de embrión de pollo. Tiene una amplia utilidad conMás información
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Número de catálogoCantidad
11150059500 mL
31150022500 mL
1115006710 × 500 mL
31150030500 mL
Número de catálogo 11150059
Precio (MXN)
966.47
Each
Añadir al carro de la compra
Cantidad:
500 mL
Customize this product
Precio (MXN)
966.47
Each
Añadir al carro de la compra
El medio 199 se desarrolló originalmente para estudios nutricionales de fibroblastos de embrión de pollo. Tiene una amplia utilidad con especies, especialmente para el cultivo de células no transformadas. El medio 199 es ampliamente utilizado en virología, producción de vacunas y cultivo in vitro de explantes primarios de epitelio pancreático de ratón y tejidos de lente de rata. Ofrecemos una gran variedad de modificaciones del medio Gibco™ 199 para diversas aplicaciones de cultivos celulares. Busque la formulación adecuada mediante la herramienta de selección de medios.

Este M199 se ha modificado de la manera siguiente:

ConSin
• Rojo de fenol• Ácido 4-(2-hidroxietil)piperazin-1-iletanosulfónico (HEPES)
• L-glutamina;

Está disponible la formulación completa.

En comparación con otros medios basales, el medio 199 contiene componentes únicos, como adenina, adenosina, hipoxantina, timina y vitaminas adicionales. El medio 199 está disponible con sales de Earle para su uso con una incubadora de CO2 o con sales de Hanks para su uso sin CO2.

Sistema de fabricación y calidad conforme a las buenas prácticas de fabricación actuales
El medio 199 Gibco™ se elabora en unas instalaciones que cumplen con las buenas prácticas de fabricación actuales ubicadas en Grand Island, Nueva York (EE. UU.). Las instalaciones se han registrado en la Agencia estadounidense de alimentos y medicamentos (FDA) como fabricante de dispositivos médicos y tienen la certificación según la norma ISO 13485. Para mantener la continuidad de la cadena de suministro, ofrecemos un medio Gibco™ 199 idéntico fabricado en nuestras instalaciones de Escocia (31150-022). Estas instalaciones se han registrado en la FDA como fabricante de dispositivos médicos y tienen la certificación según la norma ISO 13485.

El medio 199 no contiene proteínas, lípidos ni factores de crecimiento. El medio 199 utiliza un sistema de tampones de bicarbonato sódico (2,2 g/l) y, por tanto, requiere un ambiente con un 5–10 % de CO2 para mantener el pH fisiológico.

Para su uso en investigación o procesos de fabricación posteriores. No apto para uso diagnóstico ni para la administración directa en seres humanos ni en animales.

Especificaciones
Línea de célulasEpitelial de rata
Tipo de célulafibroblastos de embriones de pollo, epitelio pancreático de ratón y tejidos de lente de rata
Concentración1X
Calidad de fabricacióncGMP-compliant under the ISO 13485 standard
Línea de productosGibco
Tipo de productoMedio 199
Cantidad500 mL
Duración de almacenamiento12 meses a partir de la fecha de fabricación
Condiciones de envíoTemperatura ambiente
ClasificaciónLibre de material de origen animal
FormularioLíquido
EsterilidadEstéril con filtro
Sterilization MethodEstéril con filtro
Con aditivosBajo nivel de glucosa, Glutamina, Rojo de fenol
Sin aditivosSin HEPES, Sin piruvato sódico
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 2 a 8 °C. Proteger de la luz
Condiciones de envío: Ambiente
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

What is the shelf life of the DMEM, high glucose, pyruvate medium once the bottle is opened and the medium is supplemented?

We do not provide stability data for the product once it is opened as it would depend on the usage and storage conditions.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to store DMEM, high glucose, pyruvate medium in the dark?

Yes, the medium should be stored in the dark because there are some components in the medium such as HEPES, Tryptophan, and Riboflavin, etc. that are sensitive to light.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Medio 199, sales de Earle FAQs

Citations & References (5)

Citations & References
Abstract
Nuclear factor-kappa B directs carcinoembryonic antigen-related cellular adhesion molecule 1 receptor expression in Neisseria gonorrhoeae-infected epithelial cells.
Authors: Muenzner Petra; Billker Oliver; Meyer Thomas F; Naumann Michael;
Journal:J Biol Chem
PubMed ID:11751883
'The human-specific pathogen Neisseria gonorrhoeae expresses opacity-associated (Opa) protein adhesins that bind to various members of the carcinoembryonic antigen-related cellular adhesion molecule (CEACAM) family. In this study, we have analyzed the mechanism underlying N. gonorrhoeae-induced CEACAM up-regulation in epithelial cells. Epithelial cells represent the first barrier for the microbial pathogen. ... More
Evidence against glycogen cycling of gluconeogenic substrates in various liver preparations.
Authors: Fosgerau Keld; Breinholt Jens; McCormack James G; Westergaard Niels;
Journal:J Biol Chem
PubMed ID:12042303
'The effect of inhibition of glycogen phosphorylase by 1,4-dideoxy-1,4-imino-d-arabinitol on rates of gluconeogenesis, gluconeogenic deposition into glycogen, and glycogen recycling was investigated in primary cultured hepatocytes, in perfused rat liver, and in fed or fasted rats in vivo clamped at high physiological levels of plasma lactate. 1,4-Dideoxy-1,4-imino-d-arabinitol did not alter ... More
Guanine nucleotide exchange factor-like factor (Rlf) induces gene expression and potentiates alpha 1-adrenergic receptor-induced transcriptional responses in neonatal rat ventricular myocytes.
Authors: Post Ginell R; Swiderski Carol; Waldrop Bruce A; Salty Lina; Glembotski Christopher C; Wolthuis Rob M F; Mochizuki Naoki;
Journal:J Biol Chem
PubMed ID:11847222
Expression of constitutively active Ras (V12Ras) in cultured neonatal rat ventricular myocytes or targeted cardiac expression of V12Ras in transgenic mice induces myocardial cell growth and expression of genes that are markers of cardiac hypertrophy including atrial natriuretic factor (ANF) and myosin light chain-2. However, the signaling pathways that modulate ... More
Mechanism for peroxisome proliferator-activated receptor-alpha activator-induced up-regulation of UCP2 mRNA in rodent hepatocytes.
Authors: Nakatani Teruyo; Tsuboyama-Kasaoka Nobuyo; Takahashi Mayumi; Miura Shinji; Ezaki Osamu;
Journal:J Biol Chem
PubMed ID:11782473
Peroxisome proliferator-activated receptor-alpha (PPARalpha)activators, fish oil feeding, or fibrate administration up-regulated mitochondrial uncoupling protein (UCP2) mRNA expression in mouse liver by 5-9-fold, whereas tumor necrosis factor-alpha (TNFalpha) also up-regulated UCP2 in liver. In this study, the mechanisms for PPARalpha activators-induced up-regulation of UCP2 mRNA, related to TNFalpha and reactive oxygen ... More
Lithocholic acid decreases expression of bile salt export pump through farnesoid X receptor antagonist activity.
Authors: Yu Jinghua; Lo Jane-L; Huang Li; Zhao Annie; Metzger Edward; Adams Alan; Meinke Peter T; Wright Samuel D; Cui Jisong;
Journal:J Biol Chem
PubMed ID:12052824
Bile salt export pump (BSEP) is a major bile acid transporter in the liver. Mutations in BSEP result in progressive intrahepatic cholestasis, a severe liver disease that impairs bile flow and causes irreversible liver damage. BSEP is a target for inhibition and down-regulation by drugs and abnormal bile salt metabolites, ... More