ADN polimerasa de alta fidelidad Platinum™ Taq
ADN polimerasa de alta fidelidad Platinum&trade; <i>Taq</i>
Citas y referencias (4)
Invitrogen™

ADN polimerasa de alta fidelidad Platinum™ Taq

La ADN polimerasa de alta fidelidad Platinum™ Taq es ideal para amplificar fragmentos de ADN cuando es necesario contar conMás información
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Número de catálogoN.º de reacciones
11304029500 reacciones
11304011100 reacciones
113041025000 reacciones
Número de catálogo 11304029
Precio (MXN)
-
N.º de reacciones:
500 reacciones
Pedido a granel o personalizado
La ADN polimerasa de alta fidelidad Platinum™ Taq es ideal para amplificar fragmentos de ADN cuando es necesario contar con una producción elevada y una amplificación sólida. La alta fidelidad se obtiene mediante una mezcla de ADN polimerasa Platinum™ Taq y la enzima de corrección (actividad exonucleasa 3´→5´) de la especie Pyrococcus polimerasa GB-D. La especificidad de la PCR se ha mejorado con la incorporación de tecnología de inicio en caliente automático de Platinum™. Características de la ADN polimerasa de alta fidelidad Platinum™ Taq:

• Fidelidad: más de seis veces mayor fidelidad que la ADN polimerasa Taq
• Tamaño del amplicón: amplificación de fragmentos de hasta 15 kb (consulte la figura)
• Comodidad: conjunto de reacción a temperatura ambiente
• Aplicaciones: amplificación de ADN de moldes genómicos, virales y plasmídicos complejos; y RT-PCR

Definición de unidad
Una unidad de ADN polimerasa Platinum™ Taq, de alta fidelidad, es la cantidad de enzima necesaria para incorporar 10 nmoles de desoxirribonucleótido en ADN en 30 min a 74 °C.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Fidelidad (frente a Taq)6X
Inicio en calienteInicio en caliente integrado
N.º de reacciones500 reacciones
SobranteMezcla
PolimerasaADN polimerasa de alta fidelidad Platinum Taq
Tipo de productoADN polimerasa de alta fidelidad
Cantidad500 reacciones
Formato de reacciónComponentes separados
Condiciones de envíoHielo seco
Tamaño (producto final)20 kb o menos
Método de detecciónSonda de cebado
Para utilizar con (aplicación)Hot-start PCR, High-fidelity PCR
GC-Rich PCR PerformanceBajo
Velocidad de reacciónEstándar
Unit SizeEach
Contenido y almacenamiento
• 1 × 100 μl de ADN polimerasa Platinum Taq de alta fidelidad (5 u/µl)
• 1 × 2,25 ml del tampón de alta fidelidad 10X [600 mM de Tris-SO4 (con un pH de 8,9), 180 mM de (NH4)2SO4]
• 1 × 1 ml de MgSO4 de 50 mM

Almacenar a una temperatura de entre - 10 °C y - 30 °C.

Preguntas frecuentes

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all ADN polimerasa de alta fidelidad Platinum™ Taq FAQs

Citations & References (4)

Citations & References
Abstract
Relation between kidney function, proteinuria, and adverse outcomes.
Authors:Hemmelgarn BR, Manns BJ, Lloyd A, James MT, Klarenbach S, Quinn RR, Wiebe N, Tonelli M
Journal:JAMA
PubMed ID:20124537
'The current staging system for chronic kidney disease is based primarily on estimated glomerular filtration rate (eGFR) with lower eGFR associated with higher risk of adverse outcomes. Although proteinuria is also associated with adverse outcomes, it is not used to refine risk estimates of adverse events in this current system.' ... More
Factor B and the mitochondrial ATP synthase complex.
Authors: Belogrudov Grigory I; Hatefi Youssef;
Journal:J Biol Chem
PubMed ID:11744738
Factor B is a subunit of the mammalian ATP synthase complex, whose existence has been controversial. This paper describes the molecular and functional properties of a recombinant human factor B, which when added to bovine submitochondrial particles depleted of their factor B restores the energy coupling activity of the ATP ... More
Human protein factory for converting the transcriptome into an in vitro-expressed proteome,.
Authors:Goshima N, Kawamura Y, Fukumoto A, Miura A, Honma R, Satoh R, Wakamatsu A, Yamamoto J, Kimura K, Nishikawa T, Andoh T, Iida Y, Ishikawa K, Ito E, Kagawa N, Kaminaga C, Kanehori K, Kawakami B, Kenmochi K, Kimura R, Kobayashi M, Kuroita T, Kuwayama H, Maruyama Y, Matsuo K, Minami K, Mitsubori M, Mori M, Morishita R, Murase A, Nishikawa A, Nishikawa S, Okamoto T, Sakagami N, Sakamoto Y, Sasaki Y, Seki T, Sono S, Sugiyama A, Sumiya T, Takayama T, Takayama Y, Takeda H, Togashi T, Yahata K, Yamada H,
Journal:Nat Methods
PubMed ID:19054851
Appropriate resources and expression technology necessary for human proteomics on a whole-proteome scale are being developed. We prepared a foundation for simple and efficient production of human proteins using the versatile Gateway vector system. We generated 33,275 human Gateway entry clones for protein synthesis, developed mRNA expression protocols for them ... More
Mutation screening in 86 known X-linked mental retardation genes by droplet-based multiplex PCR and massive parallel sequencing.
Authors:Hu H, Wrogemann K, Kalscheuer V, Tzschach A, Richard H, Haas SA, Menzel C, Bienek M, Froyen G, Raynaud M, Van Bokhoven H, Chelly J, Ropers H, Chen W
Journal:Hugo J
PubMed ID:21836662
Massive parallel sequencing has revolutionized the search for pathogenic variants in the human genome, but for routine diagnosis, re-sequencing of the complete human genome in a large cohort of patients is still far too expensive. Recently, novel genome partitioning methods have been developed that allow to target re-sequencing to specific ... More