Medio RPMI 1640
Medio RPMI 1640
Citas y referencias (44)
Gibco™

Medio RPMI 1640

El medio RPMI 1640 se desarrolló originalmente para el cultivo de células leucémicas humanas en suspensión y como monocapa. AMás información
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Número de catálogoCantidad
11875101100 mL
1187512720 x 100 mL
11875093500 mL
1187511910 × 500 mL
118750851000 mL
118751356 x 1000 mL
Número de catálogo 11875101
Precio (MXN)
434.40
Each
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Cantidad:
100 mL
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Precio (MXN)
434.40
Each
Añadir al carro de la compra
El medio RPMI 1640 se desarrolló originalmente para el cultivo de células leucémicas humanas en suspensión y como monocapa. A partir de entonces, el medio Roswell Park Memorial Institute (RPMI) 1640 se ha considerado adecuado para una gran variedad de células de mamíferos, incluidas HeLa, Jurkat, MCF-7, PC12, CMSP, astrocitos y carcinomas. Ofrecemos una gran variedad de modificaciones del medio RPMI 1640 para diversas aplicaciones de cultivos celulares. Busque la formulación adecuada mediante la herramienta de selección de medios.

Esta RPMI se ha modificado de la manera siguiente:
Con  Sin
• L-glutamina  • Ácido 4-(2-hidroxietil)piperazin-1-iletanosulfónico (HEPES)
• Rojo de fenol 

Está disponible la formulación completa.

Uso de RPMI
El medio RPMI 1640 es único en comparación con otros medios porque contiene el agente reductor glutatión y altas concentraciones de vitaminas. El medio RPMI 1640 contiene biotina, vitamina B12 y ácido paraaminobenzoico (PABA), que no se encuentran en el medio esencial mínimo de Eagle o el medio Eagle modificado de Dulbecco. Además, las vitaminas inositol y colina están presentes en concentraciones muy elevadas. El medio RPMI 1640 no contiene proteínas, lípidos ni factores de crecimiento. Por lo tanto, el medio RPMI 1640 requiere una suplementación, por lo general con un 10 % de suero fetal bovino (SFB). El medio RPMI 1640 utiliza un sistema de tampones de bicarbonato sódico (2,0 g/l) y, por lo tanto, requiere un ambiente con un 5–10 % de CO2 para mantener el pH fisiológico.

Sistema de fabricación y calidad conforme las prácticas recomendadas de fabricación actuales
El medio RPMI 1640 se fabrica en unas instalaciones conformes con las prácticas recomendadas de fabricación actuales situadas en Paisley, Escocia (Reino Unido). Las instalaciones se han registrado en la Agencia estadounidense de alimentos y medicamentos (FDA) como fabricante de dispositivos médicos y tienen la certificación según la norma ISO 13485. Para la continuidad de la cadena de suministro, podemos ofrecer un producto RPMI 1640 idéntico realizado en nuestras instalaciones de Escocia (21875-158). Estas instalaciones también están registradas en la Agencia estadounidense de alimentos y medicamentos (FDA), calificadas como punto de fabricación de dispositivos médicos y están certificadas según la norma ISO 13485.

Para su uso en investigación o procesos de fabricación posteriores. No apto para uso diagnóstico ni para la administración directa en seres humanos ni en animales.

Especificaciones
Línea de célulasHeLa, Jurkat, MCF-7, PC-12, PBMC, astrocitos y carcinomas
Tipo de célulaCélulas leucémicas
Concentración1 X
Calidad de fabricacióncGMP-compliant under the ISO 13485 standard
Línea de productosGibco
Tipo de productoMedio RPMI 1640 (medio Roswell Park Memorial Institute 1640)
Cantidad100 mL
Duración de almacenamiento12 meses a partir de la fecha de fabricación
Condiciones de envíoTemperatura ambiente
ClasificaciónLibre de material de origen animal
FormularioLíquido
Serum LevelSuplementos de suero estándar
EsterilidadEstéril con filtro
Sterilization MethodEstéril con filtro
Con aditivosGlutamina, Rojo de fenol
Sin aditivosSin HEPES, Sin piruvato sódico
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 2 a 8 °C. Proteger de la luz
Condiciones de envío: Ambiente
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

How light sensitive is RPMI 1640 media? Should I also be protecting it from LED light?

While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the density (g/L) for RPMI 1640 Medium?

We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Medio RPMI 1640 FAQs

Citations & References (44)

Citations & References
Abstract
Receptor-selective effects of endogenous RGS3 and RGS5 to regulate mitogen-activated protein kinase activation in rat vascular smooth muscle cells.
Authors:Wang Qin; Liu Min; Mullah Bashar; Siderovski David P; Neubig Richard R;
Journal:J Biol Chem
PubMed ID:12006602
Regulators of G protein signaling (RGS) proteins compose a highly diverse protein family best known for inhibition of G protein signaling by enhancing GTP hydrolysis by Galpha subunits. Little is known about the function of endogenous RGS proteins. In this study, we used synthetic ribozymes targeted to RGS2, RGS3, RGS5, ... More
Silencing of Transcription of the Human Luteinizing Hormone Receptor Gene by Histone Deacetylase-mSin3A Complex.
Authors: Zhang Ying; Dufau Maria L;
Journal:J Biol Chem
PubMed ID:12091390
'Modification of chromatin structure by histone acetylases and deacetylases is an important mechanism in modulation of eukaryotic gene transcription. The present study investigated regulation of the human luteinizing hormone receptor (hLHR) gene by histone deacetylases. Inhibition of histone deacetylases (HDACs) by trichostatin A (TSA) increased hLHR promoter activity by 40-fold ... More
Distinct Intracellular Signaling in Tumor Necrosis Factor-related Apoptosis-inducing Ligand- and CD95 Ligand-mediated Apoptosis.
Authors: Velthuis Jurjen H L; Rouschop Kasper M A; De Bont Hans J G M; Mulder Gerard J; Nagelkerke J Fred;
Journal:J Biol Chem
PubMed ID:11980895
'Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a potent inducer of apoptosis in tumor cells but not in healthy cells. Similar to CD95 ligand (CD95L), TRAIL signaling requires ligand-receptor interaction; the downstream signaling molecules, such as Fas-associated death domain and caspase-8, also seem similar. Using cells stably expressing TRAIL and ... More
Intercellular calcium signaling occurs between human osteoblasts and osteoclasts and requires activation of osteoclast P2X7 receptors.
Authors: Jørgensen Niklas R; Henriksen Zanne; Sørensen Ole H; Eriksen Erik F; Civitelli Roberto; Steinberg Thomas H;
Journal:J Biol Chem
PubMed ID:11756404
'Signaling between osteoblasts and osteoclasts is important in bone homeostasis. We previously showed that human osteoblasts propagate intercellular calcium signals via two mechanisms: autocrine activation of P2Y receptors, and gap junctional communication. In the current work we identified mechanically induced intercellular calcium signaling between osteoblasts and osteoclasts and among osteoclasts. ... More
Single-cell fluorescence resonance energy transfer analysis demonstrates that caspase activation during apoptosis is a rapid process. Role of caspase-3.
Authors: Rehm Markus; Dussmann Heiko; Janicke Reiner U; Tavare Jeremy M; Kogel Donat; Prehn Jochen H M;
Journal:J Biol Chem
PubMed ID:11964393
'Activation of effector caspases is considered to be the final step in many apoptosis pathways. We transfected HeLa cells with a recombinant caspase substrate composed of cyan and yellow fluorescent protein and a linker peptide containing the caspase cleavage sequence DEVD, and we examined the cleavage kinetics at the single-cell ... More
View all Medio RPMI 1640 citations