Medio AIM V™, líquido (calidad para investigación)
Citas y referencias (26)
Gibco™
Medio AIM V™, líquido (calidad para investigación)
Contiene L-glutamina, sulfato de estreptomicina a 50 µg/ml y sulfato de gentamicina a 10 µg/ml.Más información
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Número de catálogo
Cantidad
12055083
1000 mL
12055091
500 mL
2 opciones
Número de catálogo 12055083
Precio (MXN)
-
Cantidad:
1000 mL
Customize this product
Contiene L-glutamina, sulfato de estreptomicina a 50 µg/ml y sulfato de gentamicina a 10 µg/ml.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaMonocitos, células dendríticas, células T, hibridomas, PBMC, fibroblastos, macrófagos, células de mieloma, linfocitos
Antibióticos incluidosSulfato de estreptomicina, gentamicina
Línea de productosAIM V
Tipo de productoMedio sin suero (SFM)
Grado de pureza o calidadCalidad para investigación
Cantidad1000 mL
Condiciones de envíoTemperatura ambiente
Culture TypeCultivo de células de mamíferos
FormularioLíquido
Con aditivosGlutamina, Rojo de fenol, HSA
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (2–8°C) y proteger de la luz.
Preguntas frecuentes
What is the difference between AIM V Medium (Cat. Nos. 12055083, 12055091) and CTS AIM V SFM (Cat. Nos. 0870112DK, 0870112BK)?
The difference is in the intended usage of the two media. AIM V Medium (Cat. Nos. 12055083, 12055091) is for research use only whereas CTS AIM V SFM (Cat. Nos. 0870112DK, 0870112BK) is for research use or manufacturing of cell, gene, or tissue-based products.
Which serum-free medium would you recommend for culture and expansion of mouse T cells?
You can try using AIM V Medium (Cat. No. 12055083 or 12055091) for growth of mouse T cells. For mouse cells, we recommend adding 50 µM beta-mercaptoethanol (Cat. No. 21985023).
Low oxygen tension and autologous plasma enhance T-cell proliferation and CD49d expression density in serum-free media.
Authors: Haddad H; Papoutsakis E T;
Journal:Cytotherapy
PubMed ID:11953028
'Background As cellular immunotherapy with ex vivo expanded cells becomes more widely used to treat a variety of illnesses, optimization of culture parameters, to maximize cell production and function, is essential for continued success. The effects of reduced oxygen tension and autologous plasma on T-cell expansion, receptor expression, apoptosis, and ... More
Effects of interleukin 4 upon human tumoricidal cells obtained from patients bearing solid tumors.
Authors: Jadus M R; Good R W; Crumpacker D B; Yannelli J R;
Journal:J Leukoc Biol
PubMed ID:1991997
'The use of human interleukin 4 (IL4) in the generation of tumoricidal effector cells derived from cancer patients undergoing either interleukin 2/lymphokine activated killer cells (IL2/LAK) therapy or tumor derived activated cell (TDAC) therapy was examined in the present study. Human IL4 alone did not generate LAK cells from patients ... More
T(84) epithelial cells respond to 5-hydroxytryptamine when grown in serum-free media.
Authors: Burleigh D; Fernandes K; Perrett D;
Journal:Eur J Pharmacol
PubMed ID:10708712
The aim of this study was to establish the cause of insensitivity of T(84) human colonic epithelial cells to 5-hydroxytryptamine (5-HT). Monolayers of T(84) cells were placed in modified Ussing chambers for measurement of short-circuit current, an index of secretion. When grown in serum-supplemented media, T(84) cells gave secretory responses ... More
Generation of human autologous melanoma-specific cytotoxic T cells from tumor-involved lymph nodes.
Authors: Maeda K; Lafreniere R; McKinnon J G; Jerry L M;
Journal:Mol Biother
PubMed ID:1910625
Cytotoxic T lymphocytes (CTL) specific for autologous human melanoma have been successfully generated in vitro from tumor bearing lymph nodes without any stimulation by the autologous tumor. Tumor-involved lymph node cells (LNC) were cultured in serum free medium (AIM-V) containing 1,000 U/ml of recombinant interleukin-2. The best expansion and specific ... More
Extracellular mRNA induces dendritic cell activation by stimulating tumor necrosis factor-alpha secretion and signaling through a nucleotide receptor.
We previously demonstrated that dendritic cell (DC) pulsing with antigen-encoded mRNA resulted in the loading of both major histocompatibility complex class I and II antigen presentation pathways and the delivery of an activation signal. Coculture of mRNA-pulsed DC with T cells led to the induction of a potent primary immune ... More