Medio AIM V™, líquido (calidad para investigación)
Medio AIM V™, líquido (calidad para investigación)
Citas y referencias (27)
Gibco™

Medio AIM V™, líquido (calidad para investigación)

Contiene L-glutamina, sulfato de estreptomicina a 50 µg/ml y sulfato de gentamicina a 10 µg/ml.Más información
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Número de catálogoCantidad
12055091500 mL
120550831000 mL
Número de catálogo 12055091
Precio (MXN)
-
Cantidad:
500 mL
Customize this product
Contiene L-glutamina, sulfato de estreptomicina a 50 µg/ml y sulfato de gentamicina a 10 µg/ml.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaMonocitos, células dendríticas, células T, hibridomas, PBMC, fibroblastos, macrófagos, células de mieloma, linfocitos
Antibióticos incluidosSulfato de estreptomicina, gentamicina
Línea de productosAIM V
Tipo de productoMedio sin suero (SFM)
Grado de pureza o calidadCalidad para investigación
Cantidad500 mL
Condiciones de envíoTemperatura ambiente
Culture TypeCultivo de células de mamíferos
FormularioLíquido
EsterilidadEstéril con filtro
Con aditivosGlutamina, Rojo de fenol, HSA
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (2–8°C) y proteger de la luz.

Preguntas frecuentes

What is the difference between AIM V Medium (Cat. Nos. 12055083, 12055091) and CTS AIM V SFM (Cat. Nos. 0870112DK, 0870112BK)?

The difference is in the intended usage of the two media. AIM V Medium (Cat. Nos. 12055083, 12055091) is for research use only whereas CTS AIM V SFM (Cat. Nos. 0870112DK, 0870112BK) is for research use or manufacturing of cell, gene, or tissue-based products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free medium would you recommend for culture and expansion of mouse T cells?

You can try using AIM V Medium (Cat. No. 12055083 or 12055091) for growth of mouse T cells. For mouse cells, we recommend adding 50 µM beta-mercaptoethanol (Cat. No. 21985023).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (27)

Citations & References
Abstract
Low oxygen tension and autologous plasma enhance T-cell proliferation and CD49d expression density in serum-free media.
Authors: Haddad H; Papoutsakis E T;
Journal:Cytotherapy
PubMed ID:11953028
'Background As cellular immunotherapy with ex vivo expanded cells becomes more widely used to treat a variety of illnesses, optimization of culture parameters, to maximize cell production and function, is essential for continued success. The effects of reduced oxygen tension and autologous plasma on T-cell expansion, receptor expression, apoptosis, and ... More
Effects of interleukin 4 upon human tumoricidal cells obtained from patients bearing solid tumors.
Authors: Jadus M R; Good R W; Crumpacker D B; Yannelli J R;
Journal:J Leukoc Biol
PubMed ID:1991997
'The use of human interleukin 4 (IL4) in the generation of tumoricidal effector cells derived from cancer patients undergoing either interleukin 2/lymphokine activated killer cells (IL2/LAK) therapy or tumor derived activated cell (TDAC) therapy was examined in the present study. Human IL4 alone did not generate LAK cells from patients ... More
Dominant negative effectors of heparin affin regulatory peptide (HARP) angiogenic and transforming activities.
Authors: Bernard-Pierrot Isabelle; Delbé Jean; Rouet Vincent; Vigny Marc; Kerros Marie-Emmanuelle; Caruelle Danièle; Raulais Daniel; Barritault Denis; Courty José; Milhiet Pierre Emmanuel;
Journal:J Biol Chem
PubMed ID:12070152
'Heparin affin regulatory peptide (HARP) is an heparin-binding growth factor, highly expressed in several primary human tumors and considered as a rate-limiting angiogenic factor in tumor growth, invasion, and metastasis. Implication of this protein in carcinogenesis is linked to its mitogenic, angiogenic, and transforming activities. Recently, we have demonstrated that ... More
T(84) epithelial cells respond to 5-hydroxytryptamine when grown in serum-free media.
Authors: Burleigh D; Fernandes K; Perrett D;
Journal:Eur J Pharmacol
PubMed ID:10708712
The aim of this study was to establish the cause of insensitivity of T(84) human colonic epithelial cells to 5-hydroxytryptamine (5-HT). Monolayers of T(84) cells were placed in modified Ussing chambers for measurement of short-circuit current, an index of secretion. When grown in serum-supplemented media, T(84) cells gave secretory responses ... More
Generation of human autologous melanoma-specific cytotoxic T cells from tumor-involved lymph nodes.
Authors: Maeda K; Lafreniere R; McKinnon J G; Jerry L M;
Journal:Mol Biother
PubMed ID:1910625
Cytotoxic T lymphocytes (CTL) specific for autologous human melanoma have been successfully generated in vitro from tumor bearing lymph nodes without any stimulation by the autologous tumor. Tumor-involved lymph node cells (LNC) were cultured in serum free medium (AIM-V) containing 1,000 U/ml of recombinant interleukin-2. The best expansion and specific ... More
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