Kit de purificación de ARN TRIzol™ Plus
Kit de purificación de ARN TRIzol™ Plus
Citas y referencias (1)
Invitrogen™

Kit de purificación de ARN TRIzol™ Plus

The TRIzol Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from aMás información
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Número de catálogoCantidad
1218355550 preparaciones
Número de catálogo 12183555
Precio (MXN)
8,401.54
Each
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Cantidad:
50 preparaciones
Precio (MXN)
8,401.54
Each
Añadir al carro de la compra
The TRIzol Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast. The kit combines the strong lysis capability of TRIzol Reagent (Cat. No. 15596026), followed by a convenient and time-saving silica-cartridge purification protocol from the PureLink RNA Mini Kit (Cat. No. 12183020), to provide ultrapure total RNA typically within an hour, even from difficult samples such as fibrous or fatty tissues.

Features of this kit include:

• Combined TRIzol-based lysis and PureLink spin column-based RNA isolation technologies
• Superior lysis capability and convenient protocol

Two products in one
The TRIzol Plus RNA Purification System couples the lysis capabilities of TRIzol Reagent with the 1000 μg column-;binding capacity of the PureLink RNA Mini Kit, resulting in high RNA integrity and yield. The TRIzol Plus RNA Purification System includes protocols for a variety of sample types, including fibrous or fatty tissues and plant material. The combination of TRIzol Reagent followed by silica-cartridge purification of RNA is the recommended RNA purification method in the gene expression industry. The TRIzol Plus RNA Purification Kit provides the reagents and an optimized protocol to purify total RNA for gene expression studies using an industry recommended method.

Simple lysis to column purification protocol
TRIzol Reagent sample lysis maintains the integrity of the RNA, while disrupting cells and dissolving cell components. TRIzol Reagent also provides an immediate and highly effective inhibition of RNase activity during sample homogenization. Following lysis, RNA in the lysed sample is bound to the clear silica-based membrane in the PureLink RNA Mini Kit Spin Cartridge, washed to remove contaminants, and then eluted in RNase-Free water (Tris Buffer, pH 7.5 may also be used).
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Volumen de elución30 to 300 μL
Tipo de producto finalARN total
Para utilizar con (aplicación)Análisis de micromatrices, PCR cuantitativa en tiempo real (qPCR), PCR de transcriptasa inversa (RT-PCR), construcción de bibliotecas de ADNc, etiquetado de ácidos nucleicos, ensayos de protección de nucleasas, Northern Blotting
Compatibilidad de alto rendimientoNo compatible con alto rendimiento (manual)
Tiempo de purificación20 min
Cantidad50 preparaciones
BásculaMini
Condiciones de envíoTemperatura ambiente
Cantidad de material de partidaHasta 5 × 10^8 células, hasta 200 mg de tejido
ProducciónHasta 350 μg
Isolation TechnologyColumna de centrifugación de sílice, extracción orgánica
Tipo de muestraBacterias, células, muestras vegetales, tejido, levadura, células, muestras vegetales, tejido, levadura
Unit SizeEach
Contenido y almacenamiento
Componentes del kit:
• Reactivo TRIzol™ (100 ml)
• Tampón de lisis (125 ml)
• Tampón de lavado I (50 ml)
• Tampón de lavado II (15 ml)
• Agua sin ARNasa (15,5 ml)
• Cartuchos de centrifugación con tubos de recogida (50 cada uno)
• Tubos de recogida (50 cada uno)
• Tubos de recuperación (50 cada uno)

Almacene todos los componentes a temperatura ambiente. Se suministran suficientes reactivos para realizar 50 aislamientos de ARN.

Preguntas frecuentes

Do you have any data showing differences in RNA extraction with TRIzol Reagent, the PureLink RNA Mini Kit, ChargeSwitch Total RNA Cell Kit or the TRIzol Plus RNA Purification Kit?

Please visit our website (http://www.thermofisher.com/content/dam/LifeTech/migration/en/images/ics-organized/applications/nucleic-acid-purification/data-image/560-wide.par.83692.image.559.294.1.gif) for a graph showing purity measurements and RNA integrity number (RIN) comparison of the abovementioned kits.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

What is the difference between the TRIzol Plus RNA Purification Kit and TRIzol Reagent?

The TRIzol Plus RNA Purification Kit combines the lysis capability of TRIzol Reagent with the convenient RNA extraction technology of the silica spin columns included in the PureLink RNA Mini Kit.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

What chloroform do you recommend I use for RNA extraction with TRIzol Reagent? Are there any substitutes I can use?

We recommend using straight chloroform. No isoamyl alcohol is needed (though using chloroform:isoamyl alcohol 49:1 works without problems). You can also use chloroform with 50 ppm amylene. Alternatively, BCP (1-bromo-2 chloropropane) can be used in the place of chloroform.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

What is the smallest sample volume I can use when extracting RNA with TRIzol Reagent?

Small volumes (0.5-0.8 mL) of TRIzol Reagent have been used successfully for 10^2 to 10^5 cells, but if small volumes are used, we recommend using smaller tubes in order to have the tallest possible column of aqueous phase. The taller the column of liquid, the less likely that contamination from the interphase will occur.

Here is a protocol for isolation of RNA from small quantities of tissue (1-10 mg) or cells (100-10,000):
1. Add 800 µL TRIzol Reagent to the sample. Homogenize cells by pipetting repeatedly. Add 200 µg glycogen (Cat. No. 10814010) directly to the TRIzol Reagent. If processing tissue, pulverize in liquid nitrogen first and then add 800 µL TRIzol Reagent containing 200 µg glycogen (final concentration 250 µg/mL) followed by vigorous vortexing or power homogenization.
2. Place at room temperature, cap the vial, and vortex at high speed for 10 seconds. Make sure the TRIzol Reagent wets the side of the vial in order to solubilize any sample that may be remaining on the walls.
3. Shear the genomic DNA in the sample by passing twice through a 26-gauge needle connected to a 1 mL syringe. Using the syringe, transfer the sample to a sterile 1.5 mL microcentrifuge tube.
4. Add 160 µL of chloroform (or 49:1 chloroform:isoamyl alcohol) to each sample and vortex up to 30 seconds. Centrifuge at maximum speed in a microcentrifuge for 5 minutes to separate the phases.
5. Transfer the upper aqueous phase to a fresh tube and add 400 µL ice-cold isopropanol. Allow the samples to precipitate at -20 degrees C for 1 hour to overnight. Pellet the RNA by centrifugation at maximum speed in the microfuge for 15 minutes at room temperature.
6. Decant the supernatant. Wash the pellet in 200 µL of 70% ethanol and centrifuge again for 10 minutes at maximum speed. Decant the supernatant, removing as much as possible without disturbing the pellet. Dry the RNA pellet.
7. Resolubilize the pellet in 30-50 µL RNAse-free deionized water. If tissue is high in RNAses (e.g., adrenal gland, pancreas), resuspend in 100% deionized formamide. Be sure to vortex or pipette the sample up and down to ensure that the pellet is fully resolubilized. Store at -70 degrees C.

Find additional tips, troubleshooting help, and resources within ourRNA Sample Collection, Protection, and Isolation Support Center.

What are possible stopping points and storage for RNA extraction when using TRIzol Reagent for RNA extraction? How should I store the RNA?

There are several possible stopping points and recommended storage conditions during the extraction of RNA with TRIzol Reagent:

- Sample homogenization step: After homogenization (before addition of chloroform), you can store samples at -70 degrees C for at least 1 year. The homogenated samples can sit at room temperature for several hours before adding chloroform.
- Sample homogenization step: If samples are efficiently lysed in TRIzol Reagent and the reagent inactivates the nucleases, you can safely store RNA for 3-4 days at room temperature.
- RNA precipitation step: You can store RNA in isopropanol overnight at 4 degrees C. Prolonged storage at this reduced temperature will not influence the yield of RNA appreciably. Do not store at -20 degrees C, as salts will precipitate, and do not store for a prolonged time at room temperature because the guanidine isothiocyanate can harm the RNA.
- RNA wash step: You can store RNA in 75% ethanol for 1 week at 4 degrees C or 1 year at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

View all Kit de purificación de ARN TRIzol™ Plus FAQs

Citations & References (1)

Citations & References
Abstract
Collection and storage of human blood and adipose for genomic analysis of clinical samples.
Authors:Cashion AK, Umberger RA, Goodwin SB, Sutter TR,
Journal:Res Nurs Health
PubMed ID:21812005
In this methods article, we describe collection and storage of clinically acquired blood and adipose samples for transcript analysis in an ongoing study exploring obesity in renal transplant recipients. Total ribonucleic acid (RNA) was isolated from whole blood using the LeukoLOCK™ Total RNA Isolation System (n?=?4), and comparisons between fresh ... More