Citas y referencias (3)

Gibco™

Reactivo de transfección 293fectin™

Name: Reactivo de transfección 293fectin™
SKU: 12347019

293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells.

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Número de catálogo	Cantidad
12347019	1 ml
12347500 también denominado 12347-500	15 mL
12347750 también denominado 12347-750	10 x 15 ml

3 opciones

Número de catálogo 12347019

Precio (MXN)

Cantidad:

1 ml

293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells. This reagent is optimized for transfecting suspension 293 human embryonic kidney cells (e.g., FreeStyle™ 293-F cells, Cat. No. R790-07) in defined, serum-free FreeStyle™ 293 Expression Medium (12338-018), and is intended for use with the FreeStyle™ 293 Expression System (K9000-01).

293fectin™ Transfection Reagent provides the following advantages:

Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells
Suspension FreeStyle™ 293-F cells may be transfected in FreeStyle™ 293 Expression Medium; no medium change is required
Add 293fectin™ reagent complexes directly to cells in culture medium
No need to remove complexes or change or add medium following transfection

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Para utilizar con (aplicación)Transfección

Compatibilidad de alto rendimientoNo compatible con alto rendimiento (manual)

Línea de productos293fectin

Tipo de productoReactivos para transfección

Cantidad1 ml

Compatible con sueroSí

Tipo de célulaLíneas de células establecidas

Tipo de muestraADN plasmídico

Transfection TechniqueTransfección basada en lípidos

Unit SizeEach

Contenido y almacenamiento

Cada unidad contiene un vial de reactivo. Almacenar a +4 °C. Se garantiza que 293fectin™ permanece estable durante 6 meses cuando se almacena correctamente.

Preguntas frecuentes

What is the advantage of FreeStyle Max Reagent over 293fectin Transfection Reagent?

Both FreeStyle Max Reagent and 293fectin Transfection Reagent provide similar levels of high transfection efficiency; however, FreeStyle Max Reagent has lower cytotoxicity and hence results in higher protein yields. Additionally, FreeStyle Max Reagent is animal-origin free.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

View all Reactivo de transfección 293fectin™ FAQs

Citations & References (3)

Citations & References

Abstract

Molecular dissection of the alpha-dystroglycan- and integrin-binding sites within the globular domain of human laminin-10.

Authors:Ido H, Harada K, Futaki S, Hayashi Y, Nishiuchi R, Natsuka Y, Li S, Wada Y, Combs AC, Ervasti JM, Sekiguchi K,

Journal:J Biol Chem

PubMed ID:14701821

'The adhesive interactions of cells with laminins are mediated by integrins and non-integrin-type receptors such as alpha-dystroglycan and syndecans. Laminins bind to these receptors at the C-terminal globular domain of their alpha chains, but the regions recognized by these receptors have not been mapped precisely. In this study, we sought ... More

Binding of anthrax toxin to its receptor is similar to alpha integrin-ligand interactions.

Authors:Bradley KA, Mogridge J, Jonah G, Rainey A, Batty S, Young JA,

Journal:J Biol Chem

PubMed ID:14507921

The secreted protein toxin produced by Bacillus anthracis contributes to virulence of this pathogen and can cause many of the symptoms seen during an anthrax infection, including shock and sudden death. The cell-binding component of anthrax toxin, protective antigen, mediates entry of the toxin into cells by first binding directly ... More

T cell immunoglobulin mucin-3 crystal structure reveals a galectin-9-independent ligand-binding surface.

Authors:Cao E, Zang X, Ramagopal UA, Mukhopadhaya A, Fedorov A, Fedorov E, Zencheck WD, Lary JW, Cole JL, Deng H, Xiao H, Dilorenzo TP, Allison JP, Nathenson SG, Almo SC,

Journal:Immunity

PubMed ID:17363302

The T cell immunoglobulin mucin (Tim) family of receptors regulates effector CD4(+) T cell functions and is implicated in autoimmune and allergic diseases. Tim-3 induces immunological tolerance, and engagement of the Tim-3 immunoglobulin variable (IgV) domain by galectin-9 is important for appropriate termination of T helper 1-immune responses. The 2 ... More