Fetal Bovine Serum, qualified, Canada
Fetal Bovine Serum, qualified, Canada
Gibco™

Fetal Bovine Serum, qualified, Canada

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Los sueros bovinos fetales Gibco ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayosMás información
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Número de catálogoCantidadFormato
12483020500 mLFrasco(s)
Our improved Value Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.
Número de catálogo 12483020
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Los sueros bovinos fetales Gibco ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayos específicos, ganando la confianza de los investigadores con una calidad uniforme y un soporte galardonado que ayuda a satisfacer sus necesidades de investigación y presupuesto.

Categoría de los sueros:Valor (estándar)
Origen: Canadá
Nivel de endotoxinas: ≤50 EU/ml (niveles habituales ≤10 EU/ml)
Nivel de hemoglobina: ≤25 mg/dl

Frasco libre de alícuotas de 50 ml
El frasco Gibco One Shot de 50 ml está diseñado para eliminar la necesidad de dividir en alícuotas, y el riesgo de contaminación y variabilidad que conlleva, mejorando sus posibilidades de obtener resultados consistentes.

Las características incluyen:
• Boca ancha, borde biselado y forma curva para facilitar el vertido y el pipeteo
• Diseñado para congelar/descongelar sin dividir ni romper
• Se ajusta a las gradillas de tubos de ensayo y libera espacio en el congelador
• No hay contratiempos de etiquetado, ya que la etiqueta de nuestro envase fácil es de fácil lectura y contiene toda la información que necesita
• 33 % menos de residuos generados que la división en alícuotas a partir de frascos de 500 ml

Todos los productos pueden no estar disponibles en todas las regiones debido a las regulaciones de importación. Póngase en contacto con su representante de ventas local para obtener información sobre la disponibilidad del producto en su país.
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Especificaciones
Grado de pureza o calidadCalidad para investigación, cualificado
Condiciones de envíoCongelado
EspecieGanado/bovino
AgeFetal
País de origenCanadá
FormatoFrasco(s)
Tipo de productoFetal Bovine Serum
Cantidad500 mL
Serum TreatmentEstándar (esterilizado mediante filtro)
EsterilidadEstéril
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: ≤10 °C
Condiciones de envío: Congelado

Preguntas frecuentes

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can serum be stored at 2-8 degrees C?

Serum can be kept at 2-8 degrees C for up to 4 weeks.

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

If my FBS arrives partially thawed, can I still use it?

Our studies have shown that short-term storage of thawed FBS at 4 degrees C for up to 28 days causes no decrease in growth or viability performance. FBS stored at 4 degrees C for longer periods of time should be evaluated on a case-by-case basis should this storage condition be necessary.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Sometimes I notice flocculence in serum. What could it be?

Flocculence may appear in FBS for a variety of reasons. The most common reason is the denaturation of serum lipoproteins. You may observe fibrin, one of the clot-forming proteins present in serum, after the serum has been thawed. This should not affect product performance. To remove the flocculence, transfer the serum to sterile tubes and centrifuge the material briefly at 400 x g. Then filter the resulting supernant along with your media. Do not attempt to filter serum containing flocculence, it may clog filters.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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