Lipofectamine™ 2000 CD Transfection Reagent

El reactivo de transfección (definido químicamente) Lipofectamine™ 2000 CD es una formulación patentada libre de origen animal para transfectar ácidosMás información

Número de catálogo	Cantidad
12566014	1 ml

Número de catálogo 12566014

Precio (MXN)

Cantidad:

El reactivo de transfección (definido químicamente) Lipofectamine™ 2000 CD es una formulación patentada libre de origen animal para transfectar ácidos nucleicos en células eucariotas. La utilización del reactivo Lipofectamine™ 2000 CD para la transfección proporciona las siguientes ventajas:

• La más alta eficacia de transfección en muchos tipos y formatos de células.
• La formulación libre de origen animal garantiza que los cultivos de células de mamíferos y los procesos de bioproducción estén libres de materiales de origen animal.
• Los complejos de ADN-Lipofectamine™ 2000 CD se pueden añadir directamente a las células del medio de cultivo.
• No es necesario extraer complejos ni cambiar o añadir el medio después de la transfección, pero los complejos pueden eliminarse pasadas entre cuatro y seis horas.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ClasificaciónLibre de material de origen animal, definido químicamente

Para utilizar con (aplicación)Transfección

Compatibilidad de alto rendimientoNo compatible con alto rendimiento (manual)

Línea de productosLipofectamine

Tipo de productoReactivos para transfección

Cantidad1 ml

Compatible con sueroSí

Condiciones de envíoHielo húmedo

Tipo de célulaLíneas de células establecidas

Tipo de muestraADN plasmídico

Transfection TechniqueTransfección basada en lípidos

Unit SizeEach

Contenido y almacenamiento

Cada reactivo debe almacenarse a entre 2 y 8 °C tras la recepción. Se garantiza la estabilidad de los reactivos durante seis meses si se almacenan adecuadamente.

Preguntas frecuentes

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between Lipofectamine 2000 and Lipofectamine 2000 CD?

The CD designation for Lipofectamine 2000 CD was originally provided to ensure this reagent is animal origin-free. However, all of our premium Lipofectamine lipid-based transfection reagents are animal origin-free unless stated otherwise. Please contact Technical Support for information or questions regarding any of our reagents.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

View all Lipofectamine™ 2000 CD Transfection Reagent FAQs