Citas y referencias (5)

Gibco™

Medio Advanced RPMI 1640

Name: Medio Advanced RPMI 1640
SKU: 12633020
Price: 11324.30 MXN

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cellsMás información

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Número de catálogo	Cantidad
12633020	10 x 500 mL
12633012	500 mL

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Número de catálogo 12633020

Precio (MXN)

11,324.30

Each

Cantidad:

10 x 500 mL

Customize this product

Precio (MXN)

11,324.30

Each

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.ⁱ Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.

This Advanced RPMI 1640 is manufactured as follows:

With: glucose, non-essential amino acids, sodium pyruvate, phenol red

Without: L-glutamine, HEPES

The complete formulation is available.

Gibco Advanced RPMI 1640 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Advanced RPMI 1640 requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX supplement. Many cell lines do not require adaptation to this medium. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced RPMI 1640 uses a sodium bicarbonate buffer system (2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

ⁱAdvanced Media: Reduced Sera Solutions. Quest, 2004. 1.3:26-30.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Línea de célulasSp2, Vero, Raji, Daudi y Jurkat

Concentración1 X

Calidad de fabricacióncGMP-compliant under the ISO 13485 standard

Línea de productosGibco

Tipo de productoMedio RPMI 1640 (medio Roswell Park Memorial Institute 1640)

Cantidad10 x 500 mL

Duración de almacenamiento12 meses a partir de la fecha de fabricación

ClasificaciónOrigen animal

FormularioLíquido

Serum LevelSuero reducido

EsterilidadEstéril con filtro

Sterilization MethodEstéril con filtro

Con aditivosGlucosa, Rojo de fenol, Piruvato sódico, Aminoácidos no esenciales

Sin aditivosSin glutamina, Sin HEPES

Unit SizeEach

Contenido y almacenamiento

Condiciones de almacenamiento: De 2 a 8 °C. Proteger de la luz
Condiciones de envío: Ambiente
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

What is the glucose concentration in Advanced RPMI 1640 Medium (Cat. No. 12633012, 12633020)?

Advanced RPMI 1640 Medium (Cat. No. 12633012, 12633020) contains glucose (D-Glucose or Dextrose) at a concentration of 2 g/L.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer Advanced RPMI 1640 Medium (Cat. No. 12633012 or 12633020) without phenol red?

We do not offer Advanced RPMI 1640 medium without phenol red as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Medio Advanced RPMI 1640 FAQs

Citations & References (5)

Citations & References

Abstract

Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids.

Authors:Freedman BS,Brooks CR,Lam AQ,Fu H,Morizane R,Agrawal V,Saad AF,Li MK,Hughes MR,Werff RV,Peters DT,Lu J,Baccei A,Siedlecki AM,Valerius MT,Musunuru K,McNagny KM,Steinman TI,Zhou J,Lerou PH,Bonventre JV

Journal:Nature communications

PubMed ID:26493500

Human-pluripotent-stem-cell-derived kidney cells (hPSC-KCs) have important potential for disease modelling and regeneration. Whether the hPSC-KCs can reconstitute tissue-specific phenotypes is currently unknown. Here we show that hPSC-KCs self-organize into kidney organoids that functionally recapitulate tissue-specific epithelial physiology, including disease phenotypes after genome editing. In three-dimensional cultures, epiblast-stage hPSCs form spheroids ... More

Viral complementation allows HIV-1 replication without integration.

Authors:Gelderblom HC,Vatakis DN,Burke SA,Lawrie SD,Bristol GC,Levy DN

Journal:Retrovirology

PubMed ID:18613957

BACKGROUND: The integration of HIV-1 DNA into cellular chromatin is required for high levels of viral gene expression and for the production of new virions. However, the majority of HIV-1 DNA remains unintegrated and is generally considered a replicative dead-end. A limited amount of early gene expression from unintegrated DNA ... More

The G-Protein-Coupled Estrogen Receptor Agonist G-1 Inhibits Proliferation and Causes Apoptosis in Leukemia Cell Lines of T Lineage.

Authors:Torres-López L,Olivas-Aguirre M,Villatoro-Gómez K,Dobrovinskaya O

Journal:Frontiers in cell and developmental biology

PubMed ID:35237599

The G-protein–coupled estrogen receptor (GPER) mediates non-genomic action of estrogen. Due to its differential expression in some tumors as compared to the original healthy tissues, the GPER has been proposed as a therapeutic target. Accordingly, the non-steroidal GPER agonist G-1, which has often demonstrated marked cytotoxicity in experimental models, has ... More

Drug screening of cancer cell lines and human primary tumors using droplet microfluidics.

Authors:Wong AH,Li H,Jia Y,Mak PI,Martins RPDS,Liu Y,Vong CM,Wong HC,Wong PK,Wang H,Sun H,Deng CX

Journal:Scientific reports

PubMed ID:28831060

Precision Medicine in Oncology requires tailoring of therapeutic strategies to individual cancer patients. Due to the limited quantity of tumor samples, this proves to be difficult, especially for early stage cancer patients whose tumors are small. In this study, we exploited a 2.4 × 2.4 centimeters polydimethylsiloxane (PDMS) based microfluidic chip which ... More

Nephron organoids derived from human pluripotent stem cells model kidney development and injury.

Authors:Morizane R,Lam AQ,Freedman BS,Kishi S,Valerius MT,Bonventre JV

Journal:Nature biotechnology

PubMed ID:26458176

Kidney cells and tissues derived from human pluripotent stem cells (hPSCs) would enable organ regeneration, disease modeling, and drug screening in vitro. We established an efficient, chemically defined protocol for differentiating hPSCs into multipotent nephron progenitor cells (NPCs) that can form nephron-like structures. By recapitulating metanephric kidney development in vitro, ... More