Citas y referencias (8)

Gibco™

DMEM/F-12 avanzado

Name: DMEM/F-12 avanzado
SKU: 12634028

El DMEM/F-12 avanzado (medio Eagle modificado de Dulbecco/F-12 de Ham) se utiliza por lo general como medio basal que permiteMás información

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Número de catálogo	Cantidad
12634028	10 x 500 mL
12634010	500 mL

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Número de catálogo 12634028

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10 x 500 mL

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El DMEM/F-12 avanzado (medio Eagle modificado de Dulbecco/F-12 de Ham) se utiliza por lo general como medio basal que permite el cultivo de células de mamíferos con suplementación de suero fetal bovino (SFB) reducido. En comparación con el DMEM/F-12 clásico, la suplementación sérica puede reducirse entre un 50 % y un 90 % sin ningún cambio en la tasa de crecimiento o la morfología. Entre las células cultivadas con éxito en el DMEM/F-12 avanzado, sin adaptación, se incluyen MRC-5, SP2, Vero, WI-38 y Jurkat.

Este DMEM/F-12 avanzado se ha elaborado de la manera siguiente:

Con	Sin
• Glucosa	• L-glutamina
• Aminoácidos no esenciales	• Ácido 4-(2-hidroxietil)piperazin-1-iletanosulfónico (HEPES)
• Piruvato sódico
• Rojo de fenol

Está disponible la formulación completa.

Uso del DMEM/F-12 avanzado
El medio DMEM/F-12 avanzado es distinto a otros medios debido a adición de los siguientes ingredientes para permitir la reducción de suero: etanolamina, glutatión, ácido ascórbico, insulina, transferrina, albúmina de suero bovino rica en lípidos AlbuMAX™ II para cultivo celular, y los oligoelementos selenito sódico, metavanadato amónico, sulfato cúprico y cloruro manganoso. El DMEM/F-12 avanzado requiere la suplementación con entre un 1 % y un 5 % de suero fetal bovino y 4 mM de suplemento de L-glutamina o GlutaMAX™. Muchas líneas de células no requieren adaptarse a este medio La concentración de SFB debe optimizarse para cada línea de células para obtener la máxima reducción de suero. El medio DMEM/F-12 avanzado utiliza un sistema de tampones de bicarbonato sódico (3,7 g/l) y, por tanto, requiere un ambiente con un 5–10 % de CO₂ para mantener el pH fisiológico.

Sistema de fabricación y calidad conforme con las buenas prácticas de fabricación actuales
Para mantener la continuidad de la cadena de suministro, fabricamos DMEM/F-12 avanzado en dos plantas distintas, una ubicada en Grand Island, NY (EE. UU.) y la otra en Escocia (Reino Unido). Ambos sitios cumplen los requisitos de producción según las buenas prácticas de fabricación actuales, cuentan con la certificación ISO 13485 y se han registrado en la FDA como fabricantes de dispositivos médicos.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Línea de célulasMRC-5, SP2, Vero, WI-38 y Jurkat

Concentración1 X

Calidad de fabricacióncGMP-compliant under the ISO 13485 standard

Línea de productosGibco

Tipo de productoDMEM (medio Eagle modificado de Dulbecco)/F-12

Cantidad10 x 500 mL

Duración de almacenamiento12 meses a partir de la fecha de fabricación

Condiciones de envíoTemperatura ambiente

ClasificaciónOrigen animal

FormularioLíquido

Serum LevelSuero reducido

EsterilidadEstéril con filtro

Sterilization MethodEstéril con filtro

Con aditivosAlto contenido en glucosa, Rojo de fenol, Piruvato sódico, Aminoácidos no esenciales

Sin aditivosSin glutamina, Sin HEPES

Unit SizeEach

Contenido y almacenamiento

Condiciones de almacenamiento: De 2 a 8 °C. Proteger de la luz
Condiciones de envío: Ambiente
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

Can NSCs generated using PSC Neural Induction Medium be expanded in the PSC Neural Induction Medium or do they need to be in the recommended NSC expansion medium, as stated in the product manual?

We recommend using neural expansion medium with the following composition to expand NSCs generated using the PSC Neural Induction Medium: - 50:50 Neurobasal medium: Advanced DMEM/F12 plus PSC Neural Induction Supplement.

PSC lines can behave differently, and we recommend performing your own validation experiments if you want to use PSC Neural Induction Medium, or you do not observe good expansion using the Neurobasal and Advanced DMEM/F12 combination.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer DMEM/F12 medium without folic acid?

We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all DMEM/F-12 avanzado FAQs

Citations & References (8)

Citations & References

Abstract

In vitro generation of human pluripotent stem cell derived lung organoids.

Authors:Dye BR,Hill DR,Ferguson MA,Tsai YH,Nagy MS,Dyal R,Wells JM,Mayhew CN,Nattiv R,Klein OD,White ES,Deutsch GH,Spence JR

Journal:eLife

PubMed ID:25803487

Recent breakthroughs in 3-dimensional (3D) organoid cultures for many organ systems have led to new physiologically complex in vitro models to study human development and disease. Here, we report the step-wise differentiation of human pluripotent stem cells (hPSCs) (embryonic and induced) into lung organoids. By manipulating developmental signaling pathways hPSCs ... More

Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium.

Authors:Sato T,Stange DE,Ferrante M,Vries RG,Van Es JH,Van den Brink S,Van Houdt WJ,Pronk A,Van Gorp J,Siersema PD,Clevers H

Journal:Gastroenterology

PubMed ID:21889923

Background & aims: We previously established long-term culture conditions under which single crypts or stem cells derived from mouse small intestine expand over long periods. The expanding crypts undergo multiple crypt fission events, simultaneously generating villus-like epithelial domains that contain all differentiated types of cells. We have adapted the culture ... More

Organoid cultures recapitulate esophageal adenocarcinoma heterogeneity providing a model for clonality studies and precision therapeutics.

Authors:Li X,Francies HE,Secrier M,Perner J,Miremadi A,Galeano-Dalmau N,Barendt WJ,Letchford L,Leyden GM,Goffin EK,Barthorpe A,Lightfoot H,Chen E,Gilbert J,Noorani A,Devonshire G,Bower L,Grantham A,MacRae S,Grehan N,Wedge DC,Fitzgerald RC,Garnett MJ

Journal:Nature communications

PubMed ID:30061675

Esophageal adenocarcinoma (EAC) incidence is increasing while 5-year survival rates remain less than 15%. A lack of experimental models has hampered progress. We have generated clinically annotated EAC organoid cultures that recapitulate the morphology, genomic, and transcriptomic landscape of the primary tumor including point mutations, copy number alterations, and mutational ... More

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.

Authors:Sato T,Vries RG,Snippert HJ,van de Wetering M,Barker N,Stange DE,van Es JH,Abo A,Kujala P,Peters PJ,Clevers H

Journal:Nature

PubMed ID:19329995

The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while ... More

In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration.

Authors:Huch M,Dorrell C,Boj SF,van Es JH,Li VS,van de Wetering M,Sato T,Hamer K,Sasaki N,Finegold MJ,Haft A,Vries RG,Grompe M,Clevers H

Journal:Nature

PubMed ID:23354049

The Wnt target gene Lgr5 marks actively dividing stem cells in Wnt-driven, self-renewing tissues such as small intestine and colon(1), stomach(2) and hair follicles(3). A 3D culture system allows long-term clonal expansion of single Lgr5(+) stem cells into transplantable organoids that retain many characteristics of the original epithelial architecture(2, 4, ... More

View all DMEM/F-12 avanzado citations