Fetal Bovine Serum, mesenchymal stem cell-qualified, USDA-approved regions
Fetal Bovine Serum, mesenchymal stem cell-qualified, USDA-approved regions
Gibco™

Fetal Bovine Serum, mesenchymal stem cell-qualified, USDA-approved regions

Los sueros bovinos fetales Gibco™ ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayosMás información
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Número de catálogoCantidad
1266200250 mL
12662029500 mL
Número de catálogo 12662002
Precio (MXN)
-
Cantidad:
50 mL

Los sueros bovinos fetales Gibco™ ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayos específicos, ganando la confianza de los investigadores con una calidad uniforme y un soporte galardonado que ayuda a satisfacer sus necesidades de investigación y presupuesto

Categoría de los sueros:Especialidad
Especialmente probado para la capacidad de soportar la expansión y enumeración clonal (ensayo de fibroblastos de unidad formadora de colonias) de células madre mesenquimales.
Origen:Países que cumplen los requisitos de importación del USDA.
Nivel de endotoxinas:≤50 UE/ml (niveles habituales ≤10 UE/ml).
Nivel de hemoglobina:≤25 mg/dl.
Almacenamiento: Almacenar entre -5 °C y -20 °C.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Especificaciones
Tipo de cultivoAdherent Cell Culture
Grupo de donantesNo
Concentración de endotoxinas<50 EU/mL
Concentración de hemoglobina≤25 mg/dL
Línea de productosOne Shot
Grado de pureza o calidadMS Cell-Qualified
Condiciones de envíoFrozen
EspecieCattle/Bovine
Probado paraBluetongue
AgeFetal
País de origenCountries meeting USDA importation requirements
FormularioLíquido
FormatoBottle
Tipo de productoFetal Bovine Serum
Cantidad50 mL
Serum TreatmentStandard (Sterile-filtered)
EsterilidadEstéril
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: ≤10 °C
Condiciones de envío: Congelado

Preguntas frecuentes

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and refreeze serum?

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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