Tripsina-EDTA (0,5 %), sin rojo de fenol
Tripsina-EDTA (0,5 %), sin rojo de fenol
Tripsina-EDTA (0,5 %), sin rojo de fenol
Tripsina-EDTA (0,5 %), sin rojo de fenol
Gibco™

Tripsina-EDTA (0,5 %), sin rojo de fenol

Gibco Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestiveMás información
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Número de catálogoCantidad
15400054100 mL
Número de catálogo 15400054
Precio (MXN)
-
Cantidad:
100 mL
Customize this product
Gibco Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin solution is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. Concentrated forms of trypsin can be aseptically diluted to 1X using a balanced salt solution without calcium and magnesium. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco trypsin solutions and recommended dissociation conditions.

We offer a variety of trypsin formulations and animal origin-free TrypLE reagent that feature:
• Quality testing
• Documented traceability
• Dual-site cGMP Manufacturing

This trypsin is modified as follows:

With
• EDTA

Without
• Phenol Red

The complete formulation is available.

Quality testing
Gibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, the raw materials are verified for e-beam irradiation and precursor lots are tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented traceability
We can provide detailed documentation to meet your regulatory needs. Gibco Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Gibco Trypsin-EDTA (0.5%), no phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
Para su uso en investigación o procesos de fabricación posteriores. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaCélulas de mamíferos
QuelantesEDTA
Concentración10 X
Osmolalidad300-345 mOsm/kg
Línea de productosGibco
Cantidad100 mL
Duración de almacenamiento18 meses
Condiciones de envíoHielo húmedo
FuenteOrigen animal
Probado paraBioensayo in vitro
ClasificaciónOrigen animal
FormularioLíquido
Tipo de productoReactivo para disociación de cultivo celular
EsterilidadEstéril con filtro
Con aditivosEDTA
Sin aditivosSin rojo fenol
pHDesde 7,1 hasta 8,0
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De -5 °C a -20 °C
Vida útil: 18 meses a partir de la fecha de fabricación

Preguntas frecuentes

I received Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054) with ice packs instead of dry ice and the product was partially melted. Is it OK to use?

Trypsin is shipped on wet ice as the shipment on dry ice can cause changes in pH which influences the product quality negatively. If you receive the trypsin thawed or partially thawed, the stability and functionality of the product will not be impacted. For further information please see the white paper at the following link.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the percentage of trypsin within the Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054)?

Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054 contains 0.5 % trypsin and 0.2% EDTA.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How do you recommend aliquoting Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054)?

We recommend aliquoting the undiluted trypsin into single-use portions and storing the aliquots at -20 degrees C, so that fresh dilutions can be made whenever needed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How many freeze/thaw cycles do you recommend for Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054)?

We recommend keeping the freeze thaw cycles of trypsin to a minimum to avoid loss of enzyme activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What method do you recommend for thawing Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054)?

We recommend thawing Trypsin-EDTA (0.5%) at 4 degrees C overnight. Alternatively, you can thaw it in a 37 degrees C water bath, but you will need to keep a close eye on it because extended exposure at that temperature can cause a decrease in enzyme activity. If you use the 37 degrees C thawing method, we recommend swirling the bottle every 5 mins to allow the heat to distribute evenly. As soon as the trypsin is thawed, remove it from the water bath.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (2)

Citations & References
Abstract
Isolation of multipotent neural stem or progenitor cells from both the dentate gyrus and subventricular zone of a single adult mouse.
Authors:Guo W, Patzlaff NE, Jobe EM, Zhao X,
Journal:Nat Protoc
PubMed ID:23080272
In adult mammals, the subventricular zone (SVZ) of the lateral ventricles and the subgranular zone of the dentate gyrus (DG) show ongoing neurogenesis, and multipotent neural stem or progenitor cells (NSCs) in these two regions exhibit different intrinsic properties. However, investigation of the mechanisms underlying such differences has been limited ... More
Characterization of the 46-kDa intermediates of matrix metalloproteinase 3 (stromelysin 1) obtained by site-directed mutation of phenylalanine 83.
Authors: Benbow U; Butticè G; Nagase H; Kurkinen M;
Journal:J Biol Chem
PubMed ID:8631880
The precursor of matrix metalloproteinase 3 (MMP-3/ stromelysin 1) is activated in vitro by proteinases or mercurial compounds by stepwise processes which include the initial formation of short-lived intermediates and the subsequent intermolecular cleavage of the His82-Phe83 bond to generate the fully activated mature MMP-3 (Nagase, H., Enghild, J. J., ... More