Estándar de ADN de 100 bp
Estándar de ADN de 100 bp
Citas y referencias (6)
Invitrogen™

Estándar de ADN de 100 bp

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La escalera de ADN de 100 bp Invitrogen se ha diseñado para la medición y cuantificación aproximada de ADN bicatenarioMás información
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Número de catálogoCantidad
15628050500 μl Escalera de ADN, 1 ml tampón de carga 10X
15628019100 μL Escalera de ADN, 1 ml de tampón de carga de muestras 10X, 100 aplicaciones
Número de catálogo 15628050
Precio (MXN)
-
Cantidad:
500 μl Escalera de ADN, 1 ml tampón de carga 10X
Pedido a granel o personalizado
La escalera de ADN de 100 bp Invitrogen se ha diseñado para la medición y cuantificación aproximada de ADN bicatenario en un intervalo de 100 bp a 2000 bp. La escalera de ADN de 100 bp consta de 13 fragmentos de ADN individuales purificados mediante cromatografía con bandas de referencia en 2000, 1500 y 600 bp para una orientación cómoda.

La escalera de ADN de 100 bp es idónea para la separación en geles de agarosa al 1–2 %.

Características destacadas de la escalera de ADN de 100 bp:
• Bandas nítidas y claras: fragmentos purificados mediante cromatografía para ofrecer resultados uniformes y fiables
• Cómoda: se suministra con tampón de carga de gel 10X BlueJuice para el seguimiento de la migración de ADN de la muestra
• Precisa: una cantidad exacta de ADN en cada banda

Uso del producto
La escalera de ADN bicatenaria se puede visualizar en geles de agarosa al 1–2% tras la tinción con bromuro de etidio o SYBR Safe. La escalera está diseñada con una intensidad uniforme de bandas de ADN para una visión clara de cada banda. Una cantidad exacta de ADN en cada banda permite la cuantificación aproximada de las muestras de ADN.

Esta escalera puede marcarse radiactivamente con polinucleótido quinasa T4 o ADN polimerasa T4.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración0,5 μg/μL
Compatibilidad del gelGel de agarosa
Características ecológicasEmbalaje sostenible
Contenido del kit500 μL DNA Ladder, 1 mL 10X Loading Buffer
N.º de reacciones500 aplicaciones
Tipo de productoMarcador de peso molecular
Cantidad500 μl Escalera de ADN, 1 ml tampón de carga 10X
Listo para cargarNo
Volumen de carga de muestras1 ml
Condiciones de envíoAprobado para su envío a temperatura ambiente o en hielo seco
TecnologíaFragmentos de ADN purificados por cromatografía individual
Volumen (métrico)500 µl
Tipo de gelAgarosa
Intervalo de tamañosDe 100 bp a 2000 bp
Unit SizeEach
Contenido y almacenamiento
• 500 µl 100 pb ADN Ladder
• 1 ml 10X BlueJuice Gel Loading Buffer

almacenar en -20°C.

Preguntas frecuentes

Can I know the sequences of Invitrogen DNA ladders?

Sequences of Invitrogen DNA and RNA ladders are proprietary.

Are Invitrogen DNA ladders composed of linear or circular/supercoiled DNA?

Invitrogen DNA ladders contain linear dsDNA fragments.

Are Invitrogen DNA ladders composed of single-stranded or double-stranded DNA fragments?

Invitrogen DNA ladders are composed of double-stranded DNA fragments only.

Why are the DNA bands from my molecular weight ladder smearing?

Here are a few reasons why you might see smearing of the bands:

- The DNA was degraded. Avoid nuclease contamination of DNA standards.
- Too much DNA was loaded on the gel. Decrease the amount of DNA in the gel.
- The DNA was contaminated by protein. Remove proteins by phenol extraction before electrophoresis.
- For small DNA, the bands may have diffused during staining. Add the ethidium bromide before electrophoresis.
- For radiolabeled DNA, labeling was performed by nick translation. Label the DNA by replacement synthesis with T4 DNA polymerase or label the 5' end with T4 polynucleotide kinase.
- Improper electrophoresis conditions were used. Do not allow voltage to exceed ~20 V/cm. Maintain a temperature <30°C during electrophoresis. Check that the electrophoresis buffer used has sufficient buffering capacity.
- The DNA contained too much salt. Remove excess salt by ethanol precipitation before electrophoresis.

I'm seeing anomalous migration of my DNA ladder. What happened?

This can happen if the marker was heated. Please ensure that the ladders are not heated before use.

View all Estándar de ADN de 100 bp FAQs

Citations & References (6)

Citations & References
Abstract
A duplex PCR-based assay for measuring the amount of bacterial contamination in a nucleic acid extract from a culture of free-living protists.
Authors:Marron AO, Akam M, Walker G
Journal:PLoS One
PubMed ID:23593495
'Cultures of heterotrophic protists often require co-culturing with bacteria to act as a source of nutrition. Such cultures will contain varying levels of intrinsic bacterial contamination that can interfere with molecular research and cause problems with the collection of sufficient material for sequencing. Measuring the levels of bacterial contamination for ... More
Identification and characterization of non-saccharomyces spoilage yeasts isolated from Brazilian wines.
Authors:Echeverrigaray S, Randon M, da Silva K, Zacaria J, Delamare AP
Journal:World J Microbiol Biotechnol
PubMed ID:23355138
'The industry of fine wines and also locally consumed table wines is emerging in Brazil with an increasing volume and economic impact. Enologists in this region currently lack information about the prevalence and characteristics of spoilage yeasts, which may contaminate and potentially undervalue Brazilian wines. Herein, we analyzed 50 local ... More
Systemically administered gp100 encoding DNA vaccine for melanoma using water-in-oil-in-water multiple emulsion delivery systems.
Authors:Kalariya M, Amiji MM
Journal:
PubMed ID:23702000
'The purpose of this study was to develop a water-in-oil-in-water (W/O/W) multiple emulsions-based vaccine delivery system for plasmid DNA encoding the gp100 peptide antigen for melanoma immunotherapy. The gp100 encoding plasmid DNA was encapsulated in the inner-most aqueous phase of squalane oil containing W/O/W multiple emulsions using a two-step emulsification ... More
Multiplex PCR assay targeting a diguanylate cyclase-encoding gene, cgcA, to differentiate species within the genus Cronobacter.
Authors:Carter L, Lindsey LA, Grim CJ, Sathyamoorthy V, Jarvis KG, Gopinath G, Lee C, Sadowski JA, Trach L, Pava-Ripoll M, McCardell BA, Tall BD, Hu L
Journal:Appl Environ Microbiol
PubMed ID:23144142
In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA, a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates ... More
Mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) genotyping of mycobacterium intracellulare for strain comparison with establishment of a PCR-based database.
Authors:Iakhiaeva E, McNulty S, Brown Elliott BA, Falkinham JO, Williams MD, Vasireddy R, Wilson RW, Turenne C, Wallace RJ
Journal:J Clin Microbiol
PubMed ID:23175249
Strain comparison is important to population genetics and to evaluate relapses in patients with Mycobacterium avium complex (MAC) lung disease, but the "gold standard" of pulsed-field gel electrophoresis (PFGE) is time-consuming and complex. We used variable-number tandem repeats (VNTR) for fingerprinting of respiratory isolates of M. intracellulare from patients with ... More
View all Estándar de ADN de 100 bp citations