Gentamicina (10 mg/ml)
Gentamicina (10 mg/ml)
Citas y referencias (6)
Gibco™

Gentamicina (10 mg/ml)

El sulfato de gentamicina es un antibiótico soluble en agua purificado originalmente a partir del hongo Micromonospora purpurea. La gentamicinaMás información
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Número de catálogoCantidad
1571006410 mL
1571007210 x 10 mL
Número de catálogo 15710064
Precio (MXN)
480.85
Each
Añadir al carro de la compra
Cantidad:
10 mL
Precio (MXN)
480.85
Each
Añadir al carro de la compra
El sulfato de gentamicina es un antibiótico soluble en agua purificado originalmente a partir del hongo Micromonospora purpurea. La gentamicina actúa uniéndose a la subunidad 30S del ribosoma bacteriano, lo que conduce a la inhibición de la síntesis de proteínas y a la muerte de las bacterias susceptibles. La gentamicina Gibco™ es eficaz contra una amplia variedad de bacterias grampositivas y gramnegativas, y se utiliza para la prevención de la contaminación bacteriana de cultivos celulares. La concentración de trabajo recomendada varía entre 0,5 y 50 μg/ml. Ofrecemos una amplia variedad de antibióticos y antimicóticos para aplicaciones de cultivo celular.

Uso del producto
Para uso exclusivo en investigación: no diseñado para uso diagnóstico o terapéutico en animales o humanos.

Producción según las prácticas correctas de fabricación actuales en dos instalaciones
La gentamicina Gibco™ se fabrica en una instalación que cumple con las buenas prácticas de fabricación actuales, ubicada en Grand Island, Nueva York (EE. UU.). Las instalaciones están registradas en la FDA como fabricante de dispositivos médicos y están certificadas según la norma ISO 13485. Para mantener la continuidad de la cadena de suministro, podemos ofrecer un producto de gentamicina Gibco™ comparable que fabricamos en nuestras instalaciones de Escocia (15710-049). Estas instalaciones están registradas en la FDA como fabricante de dispositivos médicos y están certificadas según la norma ISO 13485.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Concentración10 mg/mL
Tipo de cultivoCultivo de células de mamífero
Para utilizar con (aplicación)Prevención de la contaminación del cultivo celular
Cantidad10 mL
Duración de almacenamiento24 meses
Condiciones de envíoTemperatura ambiente
FormularioLíquido
Tipo de productoGentamicina
EsterilidadEstéril con filtro
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 15 a 30 °C
Condiciones de envío: Ambiente
Vida útil: 24 meses a partir de la fecha de fabricación

Preguntas frecuentes

If Gentamicin (10 mg/mL) is accidentally stored at 2-8 degrees C, would it affect the stability of the antibiotic?

No, storing Gentamicin solution for several days at 2-8 degrees C will not have any negative impact on its performance or stability. However, as Gentamicin solution has been shown to be stable at room temperature, the recommended storage temperature is ~25 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (6)

Citations & References
Abstract
Novel Cav2.1 splice variants isolated from Purkinje cells do not generate P-type Ca2+ current.
Authors: Tsunemi Taiji; Saegusa Hironao; Ishikawa Kinya; Nagayama Shin; Murakoshi Takayuki; Mizusawa Hidehiro; Tanabe Tsutomu;
Journal:J Biol Chem
PubMed ID:11756409
'The alpha(1)2.1 (alpha(1A)) subunits of P-type and Q-type Ca(2+) channels are encoded by a single gene, Cacna1a. Although these channels differ in the inactivation kinetics and sensitivity to omega-agatoxin IVA, the mechanism underlying these differences remains to be clarified. Alternative splicings of the Cacna1a transcript have been postulated to contribute ... More
Cell-autonomous impact of polysialic acid-producing enzyme ST8SIA2 on developmental migration and distribution of cortical interneurons.
Authors:Schuster UE, Rossdam C, Röckle I, Schiff M, Hildebrandt H
Journal:J Neurochem
PubMed ID:31608978
'In humans, variations in the polysialic acid-producing enzyme ST8SIA2 and disturbances in the cortical inhibitory system are associated with neurodevelopmental psychiatric disorders such as schizophrenia and autism. In mice, the ST8SIA2-dependent formation of polysialic acid during embryonic development is crucial for the establishment of interneuron populations of the medial prefrontal ... More
Identification of Novel Protein Targets of Dimethyl Fumarate Modification in Neurons and Astrocytes Reveals Actions Independent of Nrf2 Stabilization.
Authors:Piroli GG, Manuel AM, Patel T, Walla MD, Shi L, Lanci SA, Wang J, Galloway A, Ortinski PI, Smith DS, Frizzell N
Journal:Mol Cell Proteomics
PubMed ID:30587509
'The fumarate ester dimethyl fumarate (DMF) has been introduced recently as a treatment for relapsing remitting multiple sclerosis (RRMS), a chronic inflammatory condition that results in neuronal demyelination and axonal loss. DMF is known to act by depleting intracellular glutathione and modifying thiols on Keap1 protein, resulting in the stabilization ... More
Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State.
Authors:Kueck T, Bloyet LM, Cassella E, Zang T, Schmidt F, Brusic V, Tekes G, Pornillos O, Whelan SPJ, Bieniasz PD
Journal:J Virol
PubMed ID:31578292
'Interferons (IFNs) induce the expression of interferon-stimulated genes (ISGs), many of which are responsible for the cellular antiviral state in which the replication of numerous viruses is blocked. How the majority of individual ISGs inhibit the replication of particular viruses is unknown. We conducted a loss-of-function screen to identify genes ... More
Use of Precision-Cut Lung Slices as an
Authors:Rosales Gerpe MC, van Vloten JP, Santry LA, de Jong J, Mould RC, Pelin A, Bell JC, Bridle BW, Wootton SK
Journal:Mol Ther Methods Clin Dev
PubMed ID:30112421
Organotypic slice cultures recapitulate many features of an intact organ, including cellular architecture, microenvironment, and polarity, making them an ideal tool for the
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