SFB de células madre embrionarias, cualificado, formato One Shot™, de origen estadounidense
SFB de células madre embrionarias, cualificado, formato One Shot™, de origen estadounidense
Gibco™

SFB de células madre embrionarias, cualificado, formato One Shot™, de origen estadounidense

Categoría de los sueros: EspecialidadEl suero bovino fetal, válido para células madre embrionarias, se ha sometido a pruebas específicas paraMás información
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Número de catálogoCantidadFormato
16141079500 mLFrasco
1614100250 mLFrasco
Número de catálogo 16141079
Precio (MXN)
-
Cantidad:
500 mL
Formato:
Frasco
Categoría de los sueros: Especialidad
El suero bovino fetal, válido para células madre embrionarias, se ha sometido a pruebas específicas para comprobar la capacidad de mantener la morfología celular no diferenciada de las células madre embrionarias. La calidad y el rendimiento del SBF Gibco en el que usted confía en una alícuota de SBF de un solo uso, que proporciona mayor comodidad, ahorra tiempo y es fácil de usar.
Para su uso en investigación o procesos de fabricación posteriores. No apto para uso diagnóstico ni para la administración directa en seres humanos ni en animales.
Especificaciones
Grado de pureza o calidadVálido para células madre embrionarias
Condiciones de envíoCongelado
EspecieGanado/bovino
AgeFetal
País de origenEstados Unidos
FormularioLíquido
FormatoFrasco
Tipo de productoSuero fetal bovino
Cantidad500 mL
Serum TreatmentEstándar (esterilizado mediante filtro)
EsterilidadEstéril
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: ≤10 °C
Condiciones de envío: Congelado

Preguntas frecuentes

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and refreeze serum?

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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