Reactivo para transfección Lipofectamine™
Reactivo para transfección Lipofectamine™
Citas y referencias (71)
Invitrogen™

Reactivo para transfección Lipofectamine™

El reactivo de transfección Lipofectamine™ es uno de nuestros reactivos de transfección de lípidos catiónicos de primera generación formulado paraMás información
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Número de catálogoCantidad
183240204 mL
183240100,3 ml
183240121 mL
Número de catálogo 18324020
Precio (MXN)
-
Cantidad:
4 mL
El reactivo de transfección Lipofectamine™ es uno de nuestros reactivos de transfección de lípidos catiónicos de primera generación formulado para la transfección de ADN en células eucariotas. El reactivo de transfección Lipofectamine™ es un reactivo de transfección de confianza y económico con muchos años de uso citado en miles de publicaciones y con muchas líneas celulares.

Con el reactivo de transfección Lipofectamine™ obtendrá:

• Un reactivo de uso probado en aplicaciones de alto rendimiento
• Un reactivo fiable para establecer líneas celulares estables
• Un reactivo que funciona bien con el reactivo PLUS™ para obtener una mayor expresión de proteínas

Una tecnología probada
Nuestros reactivos de marca Lipofectamine™ han sido reconocidos como la gama de reactivos de transfección más citados, con decenas de miles de citas desde su lanzamiento en 1993. El reactivo de transfección Lipofectamine™ no es una excepción al constituir un reactivo de confianza para una amplia gama de líneas celulares. Cuando se utiliza en combinación con el reactivo PLUS™, se ha demostrado que el reactivo de transfección Lipofectamine™ funciona bien con células como BHK-21, NIH 3T3, COS-1, fibroblastos, queratinocitos, HT-29, MRC-5 y SK-BR3.

Una alternativa de «valor» para el ADN plasmídico
Un legado así conlleva el compromiso de proporcionar los mejores reactivos para todos los científicos y laboratorios, independientemente de sus capacidades de financiación. El reactivo de transfección Lipofectamine™ es una alternativa económica para la transfección de ADN plasmídico en células eucariotas con un protocolo simplificado. Para conseguir unos resultados óptimos en una amplia variedad de condiciones de transfección de ADN plasmídico, recomendamos el reactivo Lipofectamine™ LTX, debido a su equilibrio óptimo de potencia y baja citotoxicidad. El reactivo de transfección Lipofectamine™ 2000 se recomienda si realiza transfecciones de una amplia gama de líneas celulares o si desea transfectar ARN.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Para utilizar con (aplicación)Transfección
Compatibilidad de alto rendimientoNo compatible con alto rendimiento (manual)
Línea de productosLipofectamine
Tipo de productoReactivos para transfección
Cantidad4 mL
Compatible con sueroNo
Condiciones de envíoHielo húmedo
Tipo de célulaLíneas de células establecidas, células primarias, células difíciles de transfeccionar
FormatoPlaca de 6 pocillos, placa de 12 pocillos, placa de 24 pocillos, placa de 48 pocillos, placa de 96 pocillos, matraces
Tipo de muestraADN plasmídico
Transfection TechniqueTransfección basada en lípidos
Unit SizeEach
Contenido y almacenamiento
Contiene 4 viales (1 ml cada uno) de reactivo de transfección Lipofectamine™. Almacenar de 2–8 °C. No la congele.

Preguntas frecuentes

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

Is it necessary to use serum-free medium during lipid transfection?

It is not necessary to use serum-free medium during lipid transfection. However, it is critical to form the lipid:nucleic acid complex in the absence of serum, because proteins can interfere with complex formation. Once the complexes are formed, they can be added to cells in serum-containing medium. For optimal results with Lipofectin Transfection Reagent, we recommend performing transfection in medium without serum.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

View all Reactivo para transfección Lipofectamine™ FAQs

Citations & References (71)

Citations & References
Abstract
Low-density lipoprotein receptor-related protein 5 (LRP5) is essential for normal cholesterol metabolism and glucose-induced insulin secretion.
Authors:Fujino T, Asaba H, Kang MJ, Ikeda Y, Sone H, Takada S, Kim DH, Ioka RX, Ono M, Tomoyori H, Okubo M, Murase T, Kamataki A, Yamamoto J, Magoori K, Takahashi S, Miyamoto Y, Oishi H, Nose M, Okazaki M, Usui S, Imaizumi K, Yanagisawa M, Sakai J, Yamamoto TT,
Journal:Proc Natl Acad Sci U S A
PubMed ID:12509515
A Wnt coreceptor low-density lipoprotein receptor-related protein 5 (LRP5) plays an essential role in bone accrual and eye development. Here, we show that LRP5 is also required for normal cholesterol and glucose metabolism. The production of mice lacking LRP5 revealed that LRP5 deficiency led to increased plasma cholesterol levels in ... More
Regulation of cortical dendrite development by Slit-Robo interactions.
Authors:Whitford Kristin L; Marillat Valérie; Stein Elke; Goodman Corey S; Tessier-Lavigne Marc; Chédotal Alain; Ghosh Anirvan;
Journal:Neuron
PubMed ID:11779471
Slit proteins have previously been shown to regulate axon guidance, branching, and neural migration. Here we report that, in addition to acting as a chemorepellant for cortical axons, Slit1 regulates dendritic development. Slit1 is expressed in the developing cortex, and exposure to Slit1 leads to increased dendritic growth and branching. ... More
Extracellular Export of Sphingosine Kinase-1 Enzyme. SPHINGOSINE 1-PHOSPHATE GENERATION AND THE INDUCTION OF ANGIOGENIC VASCULAR MATURATION.
Authors:Ancellin Nicolas; Colmont Chantal; Su Joseph; Li Qin; Mittereder Nanette; Chae Sung-Suk; Stefansson Steingrimur; Liau Gene; Hla Timothy;
Journal:J Biol Chem
PubMed ID:11741921
The enzyme sphingosine kinase (SK) catalyzes the formation of sphingosine 1-phosphate (S1P), a bioactive lipid that acts extracellularly on G protein-coupled receptors of the S1P(1)/EDG-1 subfamily. Although S1P is formed in the cytosol of various cells, S1P release is not understood and is controversial because this lipid mediator is also ... More
Different composite regulatory elements direct expression of the human alpha subunit gene to pituitary and placenta.
Authors: Heckert L L; Schultz K; Nilson J H;
Journal:J Biol Chem
PubMed ID:7592867
'To identify elements of the human alpha subunit gene necessary for cell-specific expression, we generated an array of block mutations spanning approximately 400 base pairs (bp) of promoter proximal region and examined them using transient transfection analysis in pituitary (alpha T3) and placental (BeWo) cell lines. Comparison of promoter activity ... More
ATF3 gene. Genomic organization, promoter, and regulation.
Authors: Liang G; Wolfgang C D; Chen B P; Chen T H; Hai T;
Journal:J Biol Chem
PubMed ID:8576171
'ATF3 gene, which encodes a member of the activating transcription factor/cAMP responsive element binding protein (ATF/CREB) family of transcription factors, is induced by many physiological stresses. As a step toward understanding the induction mechanisms, we isolated the human ATF3 gene and analyzed its genome organization and 5''-flanking region. We found ... More
View all Reactivo para transfección Lipofectamine™ citations