CaptureSelect™ Human Growth Hormone Affinity Matrix

CaptureSelect Human Growth Hormone (hGH) Affinity Matrix has been specifically designed for the purification of human growth hormone from recombinant sources.

Features of this affinity matrix include:

• Highly selective for recombinant hGH
• Efficient purification of recombinant hGH with high yields and retained activity
• Excellent scalability
• Non-animal-derived

Recombinant hGH, also known as somatotropin or somatropin, is a peptide hormone that stimulates growth, cell reproduction, and regeneration in humans and other animals. The high selectivity and yields obtained using CaptureSelect Human Growth Hormone Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in one step.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

• Matrix: agarose-based, aldehyde-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect hGH affinity ligand
• Ligand coupling method: aldehyde coupling via NH₂ residues of the ligand
• Binding capacity: ∼3 mg/mL resin
• Elution conditions: 20 mM citric acid, pH 3.0
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20%(v/v) ethanol