IMDM, sin rojo fenol
IMDM, sin rojo fenol
Citas y referencias (3)
Gibco™

IMDM, sin rojo fenol

El medio de Dulbecco modificado de Iscove (IMDM) es muy adecuado para cultivos celulares de alta densidad y proliferación rápida,Más información
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Número de catálogoCantidad
21056023500 mL
Número de catálogo 21056023
Precio (MXN)
-
Cantidad:
500 mL
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El medio de Dulbecco modificado de Iscove (IMDM) es muy adecuado para cultivos celulares de alta densidad y proliferación rápida, como Jurkat, COS-7 y macrófagos. Ofrecemos una gran variedad de modificaciones IMDM de Gibco® para una gama de aplicaciones de cultivos celulares. Busque la formulación adecuada mediante la herramienta de selección de medios.
Este IMDM se ha modificado de la manera siguiente:
Con Sin
• L-glutamina • Rojo de fenol
• α-tioglicerol
• 2-mercaptoetanol


Está disponible la formulación completa.

El IMDM, una modificación del medio Eagle modificado de Dulbecco, incluye selenio, así como aminoácidos y vitaminas adicionales. Además, este medio único carece de hierro, con nitrato de potasio en sustitución del nitrato férrico.

Uso del producto
Solo para uso en investigación: No diseñado para uso diagnóstico o terapéutico en animales o humanos.

Sistema de fabricación y calidad conforme a las buenas prácticas de fabricación actuales
Gibco® IMDM se fabrica en unas instalaciones conforme a las buenas prácticas de fabricación actuales ubicadas en Grand Island Nueva York (EE. UU.). Estas instalaciones están registradas en la FDA como fabricante de dispositivos médicos y están certificadas según la norma ISO 13485.

IMDM no contiene proteínas, lípidos ni factores de crecimiento. Por lo tanto, el IMDM requiere suplementación, generalmente con 10 % de suero fetal bovino (SFB). IMDM utiliza un sistema de tampones de bicarbonato de sodio (3,024 g/l) y, por tanto, requiere un ambiente con un 5-10 % de CO2 para mantener el pH fisiológico.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Línea de célulasCélulas Jurkat, COS-7 y macrófagos
Concentración1 X
Calidad de fabricacióncGMP-compliant under the ISO 13485 standard
Línea de productosGibco
Tipo de productoIMDM (medio de Dulbecco modificado de Iscove)
Cantidad500 mL
Duración de almacenamiento12 meses a partir de la fecha de fabricación
Condiciones de envíoTemperatura ambiente
ClasificaciónLibre de material de origen animal
FormularioLíquido
EsterilidadEstéril con filtro
Con aditivosAlto contenido en glucosa, Glutamina, Ácido 4-(2-hidroxietil)piperazin-1-iletanosulfónico (HEPES), Piruvato sódico
Sin aditivosSin rojo fenol, Sin α-tioglicerol, Sin 2-mercaptoetanol
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 2 a 8 °C. Proteger de la luz
Condiciones de envío: Ambiente
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all IMDM, sin rojo fenol FAQs

Citations & References (3)

Citations & References
Abstract
Nongenomic testosterone calcium signaling. Genotropic actions in androgen receptor-free macrophages.
Authors:Guo Z, Benten WP, Krucken J, Wunderlich F
Journal:J Biol Chem
PubMed ID:12048191
'Steroid hormones exert genotropic actions through members of the nuclear receptor family. Here, we have demonstrated genotropic actions of testosterone that are independent of intracellular androgen receptors (iAR). Through plasma membrane androgen receptors (mAR), testosterone induces a rapid rise in the intracellular free Ca(2+) concentration of iAR-free murine RAW 264.7 ... More
The PAX3-FKHR fusion protein created by the t(2;13) translocation in alveolar rhabdomyosarcomas is a more potent transcriptional activator than PAX3.
Authors:Fredericks WJ, Galili N, Mukhopadhyay S, Rovera G, Bennicelli J, Barr FG, Rauscher FJ 3rd
Journal:Mol Cell Biol
PubMed ID:7862145
Alveolar rhabdomyosarcomas are pediatric solid tumors with a hallmark cytogenetic abnormality: translocation of chromosomes 2 and 13 [t(2;13) (q35;q14)]. The genes on each chromosome involved in this translocation have been identified as the transcription factor-encoding genes PAX3 and FKHR. The NH2-terminal paired box and homeodomain DNA-binding domains of PAX3 are ... More
Role of JunB in erythroid differentiation.
Authors: Jacobs-Helber Sarah M; Abutin Randolph M; Tian Cuixia; Bondurant Maurice; Wickrema Amittha; Sawyer Stephen T;
Journal:J Biol Chem
PubMed ID:11726656
The role of junB as a regulator of erythroid cell survival, proliferation, and differentiation was tested by controlled expression of JunB in the erythropoietin (EPO)-dependent erythroleukemia cell line HCD57. JunB induced erythroid differentiation as evidenced by increased expression of the erythroid-specific proteins beta-globin, spectrin-alpha, and TER-119. Expression of JunB for ... More