SIAB (succinimidyl (4-iodoacetyl)aminobenzoate)
SIAB (succinimidyl (4-iodoacetyl)aminobenzoate)
Citas y referencias (5)
Thermo Scientific™

SIAB (succinimidyl (4-iodoacetyl)aminobenzoate)

Thermo Scientific Pierce SIAB es un entrecruzador de longitud media (10,6 angstrom) para la conjugación de amina a sulfhidrilo aMás información
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Número de catálogoCantidad
2232950 mg
Número de catálogo 22329
Precio (MXN)
-
Cantidad:
50 mg
Thermo Scientific Pierce SIAB es un entrecruzador de longitud media (10,6 angstrom) para la conjugación de amina a sulfhidrilo a través de éster (NHS) N-hidroxisuccinimida y grupos reactivos yodoacetil.

Características de SIAB:

• Grupos reactivos: Éster NHS y yodoacetil
• Reactivo hacia: grupos amino y sulfhidrilo
• No escindible
• Insoluble en agua (disolver primero en DMF o DMSO); en comparación con Sulfo-SIAB
• Se utiliza para formar inmunoconjugados enzimáticos de IgG estables
• Forma conjugados con liposomas

Referencias del producto:
Guía de aplicación entrecruzada: búsqueda de referencias literarias recientes para este producto

Productos relacionados
Sulfo-SIAB (sulfosuccinimidil-(4-yodoacetil)aminobenzoato)
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Permeabilidad celularSí
DescripciónSIAB
FormularioPolvo
Método de etiquetadoEtiqueta química
Peso molecular402.14
PegiladoNo
Línea de productosPierce
Cantidad50 mg
Fracción reactivaHaloacetil, éster NHS
Condiciones de envíoAmbiente
SolubilidadDMF, DMSO
Longitud del brazo del separador10,6 Å
Soluble en aguaNo
Reactividad químicaAmino-sulfhidrilo
CleavableNo
Tipo de enlace cruzadoHeterobifuncionales
FormatoEstándar
Tipo de productoEntrecruzador
SeparadorMedio (de 10 a 30 Å)
Unit SizeEach
Contenido y almacenamiento
Tras su recepción, se debe almacenar a 4 °C, protegido de la luz.

Preguntas frecuentes

Can you provide the shelf-life for SIAB (succinimidyl (4-iodoacetyl)aminobenzoate)?

SIAB (succinimidyl (4-iodoacetyl)aminobenzoate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (5)

Citations & References
Abstract
Chip-based genotyping by mass spectrometry.
Authors:Tang K, Fu DJ, Julien D, Braun A, Cantor CR, Köster H
Journal:Proc Natl Acad Sci U S A
PubMed ID:10468554
Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip wells via N-succinimidyl (4-iodoacetyl)aminobenzoate (SIAB) chemistry. Primer annealing, extension, and termination were performed on a 1-microl scale directly in the chip ... More
Covalent attachment of synthetic DNA to self-assembled monolayer films.
Authors:Chrisey LA, Lee GU, O'Ferrall CE
Journal:Nucleic Acids Res
PubMed ID:8760890
'The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film ... More
Gbeta subunit interacts with a peptide encoding region 956-982 of adenylyl cyclase 2. Cross-linking of the peptide to free Gbetagamma but not the heterotrimer.
Authors:Weng G, Li J, Dingus J, Hildebrandt JD, Weinstein H, Iyengar R
Journal:J Biol Chem
PubMed ID:8900107
The region encoded by amino acids 956-982 of adenylyl cyclase 2 is important for Gbetagamma stimulation. Interactions of a peptide encoding the 956-982 region of adenylyl cyclase 2 (QEHAQEPERQYMHIGTMVEFAYALVGK (QEHA peptide)) with Gbetagamma subunits were studied. QEHA peptide was covalently attached to beta subunit of free Gbetagamma by the cross-linker ... More
Mitochondrial protein import: recognition of internal import signals of BCS1 by the TOM complex.
Authors:Stan T, Brix J, Schneider-Mergener J, Pfanner N, Neupert W, Rapaport D
Journal:Mol Cell Biol
PubMed ID:12640110
BCS1, a component of the inner membrane of mitochondria, belongs to the group of proteins with internal, noncleavable import signals. Import and intramitochondrial sorting of BCS1 are encoded in the N-terminal 126 amino acid residues. Three sequence elements were identified in this region, namely, the transmembrane domain (amino acid residues ... More
Use of immobilized PCR primers to generate covalently immobilized DNAs for in vitro transcription/translation reactions.
Authors:Andreadis JD, Chrisey LA
Journal:Nucleic Acids Res
PubMed ID:10606673
We have developed a novel biochemical method to simultaneously amplify and immobilize a target gene onto insoluble particles using PCR. This method employs the covalent attachment of one of two PCR primers to a particle surface either directly during DNA synthesis of the primer or post-DNA synthesis, through the use ... More