Medio RPMI 1640, polvo, HEPES

Citas y referencias (2)

Gibco™

Medio RPMI 1640, polvo, HEPES

Name: Medio RPMI 1640, polvo, HEPES
SKU: 23400062

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

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Número de catálogo	Cantidad
23400062	10 L
23400021	10 x1 L

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Número de catálogo 23400062

Precio (MXN)

Cantidad:

10 L

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Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications.

This RPMI is modified as follows:

With: L-glutamine, Phenol Red, HEPES

Without: Sodium Bicarbonate

The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B₁₂ and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Intended Use

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Please note that the shelf life on delivered products varies. This product has a minimum shelf life of 6 months at time of shipment. For more information regarding product shelf life, including details on specific product lots, please contact Customer Service.

Para uso exclusivo en investigación.No apto para uso en procedimientos diagnósticos.no está indicado para uso terapéutico en humanos ni en animales.Los usos que vayan más allá del uso previsto podrían infringir la legislación local.Los clientes que utilicen el medio RPMI 1640 Gibco en un proceso de fabricación y que deban presentar documentación a la FDA podrán solicitar una carta de autorización de Life Technologies en la que se haga referencia a su archivo principal sobre fármacos (DMF) de tipo II.Para uso diagnóstico in vitro.

Especificaciones

Línea de célulasHeLa, Jurkat, MCF-7, PC-12, PBMC, astrocitos y carcinomas

Tipo de célulaCélulas leucémicas

Calidad de fabricacióncGMP-compliant under the ISO 13485 standard

Línea de productosGibco

Tipo de productoMedio RPMI 1640 (medio Roswell Park Memorial Institute 1640)

Cantidad10 L

Duración de almacenamiento36 meses a partir de la fecha de fabricación

Condiciones de envíoTemperatura ambiente

ClasificaciónLibre de material de origen animal

FormularioPolvo

Serum LevelSuplementos de suero estándar

EsterilidadEstéril con filtro

Con aditivosGlutamina, Ácido 4-(2-hidroxietil)piperazin-1-iletanosulfónico (HEPES), Rojo de fenol

Sin aditivosSin piruvato sódico, Sin bicarbonato sódico

Unit SizeEach

Contenido y almacenamiento

Condiciones de almacenamiento: 2-8 °C.
Condiciones de envío: Ambiente
Vida útil: 36 meses a partir de la fecha de fabricación

Preguntas frecuentes

How light sensitive is RPMI 1640 media? Should I also be protecting it from LED light?

While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the density (g/L) for RPMI 1640 Medium?

We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of my powdered media once I reconstitute it?

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all Medio RPMI 1640, polvo, HEPES FAQs

Citations & References (2)

Citations & References

Abstract

Ultraviolet-induced junD activation and apoptosis in myeloblastic leukemia ML-1 cells.

Authors:Li T, Dai W, Lu L.

Journal:J Biol Chem

PubMed ID:12082101

'The exposure of mammalian cells to UV irradiation induces the expression of immediate early genes such as c-jun and c-fos and activates the transcription factors AP-1 and NF-kappaB. JunD is one of the three members of the Jun family and shares some functional characteristics with c-Jun. In the present study, ... More

Role for 18:1 lysophosphatidic acid as an autocrine mediator in prostate cancer cells.

Authors:Xie Y, Gibbs TC, Mukhin YV, Meier KE.

Journal:J Biol Chem

PubMed ID:12084719

Lysophosphatidic acid (LPA) is a lipid mediator that may play an important role in growth and survival of carcinomas. In this study, LPA production and response were characterized in two human prostate cancer (CaP) cell lines: PC-3 and Du145. Bombesin, a neuroendocrine peptide that is mitogenic for CaP cells, stimulated ... More