L-glutamina (200 mM)
L-glutamina (200 mM)
Citas y referencias (10)
Gibco™

L-glutamina (200 mM)

La L-glutamina es un aminoácido necesario para el cultivo celular. La L-glutamina participa en la formación de purina y nucleótidosMás información
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Número de catálogoCantidad
25030081100 mL
2503014920 mL
2503016420 x 100 mL
Número de catálogo 25030081
Precio (MXN)
-
Cantidad:
100 mL
Customize this product
La L-glutamina es un aminoácido necesario para el cultivo celular. La L-glutamina participa en la formación de purina y nucleótidos de pirimidina, aminoazúcares, glutatión, L-glutamato y otros aminoácidos, así como en la síntesis de proteínas y la producción de glucosa. A diferencia de la mayoría de los demás aminoácidos, la L-glutamina no es estable en solución. El ritmo al que avanza la degradación depende del tiempo, la temperatura y el pH. La L-glutamina Gibco™ es una solución madre de 200 mM lista para usar que actúa como un suplemento de cultivo celular. La concentración óptima depende del tipo de célula y del medio empleado para cultivar las células, pero por lo general se sitúa en el intervalo de 2 a 6 mm.

También ofrecemos el suplemento Gibco™ GlutaMAX™ como una alternativa estable a la L-glutamina.

Sistema de fabricación y calidad según las buenas prácticas de fabricación actuales en dos instalaciones
La L-glutamina Gibco™ se fabrica en unas instalaciones conformes con las buenas prácticas de fabricación actuales situadas en Paisley, Escocia (Reino Unido). Las instalaciones están registradas en la Agencia estadounidense de alimentos y medicamentos (FDA) como fabricante de dispositivos médicos y están certificadas según la norma ISO 13485. Para la continuidad de la cadena de suministro, ofrecemos un producto de L-glutamina Gibco™ idéntico fabricado en nuestras instalaciones de Grand Island (EE. UU.) (25030-081). Estas instalaciones se han registrado en la FDA como fabricante de dispositivos médicos y tienen la certificación según la norma ISO 13485.
Especificaciones
Nombre del producto químico o materialGlutamina
Concentration or Composition (by Analyte or Components)100 X
Características ecológicasEmbalaje sostenible
Forma físicaLíquido
Línea de productosGibco
Almacenamiento recomendadoCondiciones de almacenamiento: De – 5 °C a – 20 °C. Proteger de la luz
Condiciones de envío: Hielo seco
Vida útil: 24 meses a partir de la fecha de fabricación
Duración de almacenamiento24 meses
Condiciones de envíoHielo seco
Cantidad100 mL
pHentre 6 y 8
Unit SizeEach

Preguntas frecuentes

Why did my 200 mM L-Glutamine precipitate out of solution when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why does L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution have “Product of Brazil” on the label?

L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution is made in the USA from a raw material glutamine powder manufactured in Brazil. The country of manufacture of a component that has been subjected to manufacturing or processing operations in more than one country is the last country in which the component has been “substantially transformed”. A “substantial transformation” occurs in a country when a product emerges from a manufacturing process with a name, character, or use different from that of the original material(s) subjected to that process.

Diluting, purifying, sorting, testing, packing, repacking, or affixing labels to a product in a country does not constitute a “substantial transformation” in that country, hence the label must bear the country of the original raw material origin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My order of L-Glutamine (Cat. No. 25030081) arrived frozen. Is it stable?

If L-Glutamine is completely or partially frozen, it is still stable. It becomes unstable when stored for extended periods of time completely thawed, especially above 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is your recommended method for thawing L-Glutamine before adding to cell culture media?

We recommend thawing at 2-8 degrees C and then warming at 37 degrees C until the material goes into solution. Mix it as it warms up to spend minimal time at elevated temperatures before you aliquot it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why did my 200 mM L-glutamine precipitate when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37 degrees C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (10)

Citations & References
Abstract
Structural and energetic characteristics of the heparin-binding site in antithrombotic protein C.
Authors:Friedrich U, Blom AM, Dahlbäck B, Villoutreix BO,
Journal:J Biol Chem
PubMed ID:11316800
'Human activated protein C (APC) is a key component of a natural anticoagulant system that regulates blood coagulation. In vivo, the catalytic activity of APC is regulated by two serpins, alpha1-antitrypsin and the protein C inhibitor (PCI), the inhibition by the latter being stimulated by heparin. We have identified a ... More
FRET measurements of intracellular cAMP concentrations and cAMP analog permeability in intact cells.
Authors:Börner S, Schwede F, Schlipp A, Berisha F, Calebiro D, Lohse MJ, Nikolaev VO,
Journal:Nat Protoc
PubMed ID:21412271
'Real-time measurements of second messengers in living cells, such as cAMP, are usually performed by ratiometric fluorescence resonance energy transfer (FRET) imaging. However, correct calibration of FRET ratios, accurate calculations of absolute cAMP levels and actual permeabilities of different cAMP analogs have been challenging. Here we present a protocol that ... More
A critical role for choline kinase-alpha in the aggressiveness of bladder carcinomas.
Authors:Hernando E, Sarmentero-Estrada J, Koppie T, Belda-Iniesta C, Ramírez de Molina V, Cejas P, Ozu C, Le C, Sánchez JJ, González-Barón M, Koutcher J, Cordón-Cardó C, Bochner BH, Lacal JC, Ramírez de Molina A,
Journal:Oncogene
PubMed ID:19448670
'Bladder cancer is one of the most common causes of death in industrialized countries. New tumor markers and therapeutic approaches are still needed to improve the management of bladder cancer patients. Choline kinase-alpha (ChoKalpha) is a metabolic enzyme that has a role in cell proliferation and transformation. Inhibitors of ChoKalpha ... More
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.
Authors:Walev I, Bhakdi SC, Hofmann F, Djonder N, Valeva A, Aktories K, Bhakdi S,
Journal:Proc Natl Acad Sci U S A
PubMed ID:11248053
'The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10(5)-10(6) molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca(2+)-calmodulin and on ... More
Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells.
Authors:Nandivada H, Villa-Diaz LG, O'Shea KS, Smith GD, Krebsbach PH, Lahann J,
Journal:Nat Protoc
PubMed ID:21720316
The culture of human embryonic stem (hES) cells in defined and xenogeneic-free conditions will contribute substantially to future biotechnological and medical applications. To achieve this goal, we developed the first fully defined synthetic polymer coating poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH) that sustains long-term growth of hES cells in different culture media. ... More
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