L-glutamina (200 mM)

Citas y referencias (3)

Gibco™

L-glutamina (200 mM)

Name: L-glutamina (200 mM)
SKU: 25030164

La L-glutamina es un aminoácido necesario para el cultivo celular. La L-glutamina participa en la formación de purina y nucleótidosMás información

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Número de catálogo	Cantidad
25030164	20 x 100 mL
25030149	20 mL
25030081	100 mL

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Número de catálogo 25030164

Precio (MXN)

Cantidad:

20 x 100 mL

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La L-glutamina es un aminoácido necesario para el cultivo celular. La L-glutamina participa en la formación de purina y nucleótidos de pirimidina, aminoazúcares, glutatión, L-glutamato y otros aminoácidos, así como en la síntesis de proteínas y la producción de glucosa. A diferencia de la mayoría de los demás aminoácidos, la L-glutamina no es estable en solución. El ritmo al que avanza la degradación depende del tiempo, la temperatura y el pH. La L-glutamina Gibco™ es una solución madre de 200 mM lista para usar que actúa como un suplemento de cultivo celular. La concentración óptima depende del tipo de célula y del medio empleado para cultivar las células, pero por lo general se sitúa en el intervalo de 2 a 6 mm.

También ofrecemos el suplemento Gibco™ GlutaMAX™ como una alternativa estable a la L-glutamina.

Sistema de fabricación y calidad según las buenas prácticas de fabricación actuales en dos instalaciones
La L-glutamina Gibco™ se fabrica en unas instalaciones conformes con las buenas prácticas de fabricación actuales situadas en Paisley, Escocia (Reino Unido). Las instalaciones están registradas en la Agencia estadounidense de alimentos y medicamentos (FDA) como fabricante de dispositivos médicos y están certificadas según la norma ISO 13485. Para la continuidad de la cadena de suministro, ofrecemos un producto de L-glutamina Gibco™ idéntico fabricado en nuestras instalaciones de Grand Island (EE. UU.) (25030-081). Estas instalaciones se han registrado en la FDA como fabricante de dispositivos médicos y tienen la certificación según la norma ISO 13485.

Especificaciones

Nombre del producto químico o materialGlutamine

Concentration or Composition (by Analyte or Components)100X

Características ecológicasSustainable packaging

Forma físicaLíquido

Almacenamiento recomendadoStorage conditions: -5°C to -20°C. Protect from light.
Shipping conditions: Dry ice
Shelf life: 24 months from date of manufacture

Duración de almacenamiento24 Months

Cantidad20 x 100 mL

pH5 to 6

Unit SizeEach

Preguntas frecuentes

Why did my 200 mM L-Glutamine precipitate out of solution when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why does L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution have “Product of Brazil” on the label?

L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution is made in the USA from a raw material glutamine powder manufactured in Brazil. The country of manufacture of a component that has been subjected to manufacturing or processing operations in more than one country is the last country in which the component has been “substantially transformed”. A “substantial transformation” occurs in a country when a product emerges from a manufacturing process with a name, character, or use different from that of the original material(s) subjected to that process.

Diluting, purifying, sorting, testing, packing, repacking, or affixing labels to a product in a country does not constitute a “substantial transformation” in that country, hence the label must bear the country of the original raw material origin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My order of L-Glutamine (Cat. No. 25030081) arrived frozen. Is it stable?

If L-Glutamine is completely or partially frozen, it is still stable. It becomes unstable when stored for extended periods of time completely thawed, especially above 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is your recommended method for thawing L-Glutamine before adding to cell culture media?

We recommend thawing at 2-8 degrees C and then warming at 37 degrees C until the material goes into solution. Mix it as it warms up to spend minimal time at elevated temperatures before you aliquot it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why did my 200 mM L-glutamine precipitate when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37 degrees C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (3)

Citations & References

Abstract

FRET measurements of intracellular cAMP concentrations and cAMP analog permeability in intact cells.

Authors:Börner S, Schwede F, Schlipp A, Berisha F, Calebiro D, Lohse MJ, Nikolaev VO,

Journal:Nat Protoc

PubMed ID:21412271

'Real-time measurements of second messengers in living cells, such as cAMP, are usually performed by ratiometric fluorescence resonance energy transfer (FRET) imaging. However, correct calibration of FRET ratios, accurate calculations of absolute cAMP levels and actual permeabilities of different cAMP analogs have been challenging. Here we present a protocol that ... More

A critical role for choline kinase-alpha in the aggressiveness of bladder carcinomas.

Authors:Hernando E, Sarmentero-Estrada J, Koppie T, Belda-Iniesta C, Ramírez de Molina V, Cejas P, Ozu C, Le C, Sánchez JJ, González-Barón M, Koutcher J, Cordón-Cardó C, Bochner BH, Lacal JC, Ramírez de Molina A,

Journal:Oncogene

PubMed ID:19448670

'Bladder cancer is one of the most common causes of death in industrialized countries. New tumor markers and therapeutic approaches are still needed to improve the management of bladder cancer patients. Choline kinase-alpha (ChoKalpha) is a metabolic enzyme that has a role in cell proliferation and transformation. Inhibitors of ChoKalpha ... More

Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells.

Authors:Nandivada H, Villa-Diaz LG, O'Shea KS, Smith GD, Krebsbach PH, Lahann J,

Journal:Nat Protoc

PubMed ID:21720316

The culture of human embryonic stem (hES) cells in defined and xenogeneic-free conditions will contribute substantially to future biotechnological and medical applications. To achieve this goal, we developed the first fully defined synthetic polymer coating poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH) that sustains long-term growth of hES cells in different culture media. ... More