PageRuler™ Unstained High Range Protein Ladder
PageRuler™ Unstained High Range Protein Ladder
Thermo Scientific™

PageRuler™ Unstained High Range Protein Ladder

La escalera de proteínas de rango alto sin teñir Thermo Scientific PageRuler es una mezcla de ocho (8) proteínas (deMás información
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Número de catálogoCantidad
266372 × 250 ml
Número de catálogo 26637
Precio (MXN)
-
Cantidad:
2 × 250 ml
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La escalera de proteínas de rango alto sin teñir Thermo Scientific PageRuler es una mezcla de ocho (8) proteínas (de 60 a 250 kDa) para su uso como patrones de tamaño en electroforesis de proteínas (SDS-PAGE) y Western blot.

Características de la escalera de proteínas de rango alto sin teñir PageRuler:

Intervalo de tamaños: ocho proteínas con un tamaño de 60 a 250 kDa
Producto listo para su uso: se suministra en tampón de carga para su carga directa en geles
Bandas nítidas: con una excelente precisión para su detección con tinciones de plata o coomassie
Banda de referencia: la banda de 150 kDa tiene mayor intensidad para facilitar la orientación
Etiquetado: cada una de las proteínas de la escalera contiene una secuencia integral Strep-tag™ II
Calidad probada: cada lote se evalúa mediante SDS-PAGE y Western blot

Los marcadores de peso molecular de proteínas en esta escalera se resuelven en bandas nítidas claramente identificables cuando se analizan mediante SDS-PAGE y se tiñen con colorante de coomassie o plata. La banda de 150 kDa es de mayor intensidad y sirve como banda de referencia. Las proteínas se pueden detectar en western blots mediante tinción con colorantes de Ponceau S, azul de Coomassie u otros colorantes para proteínas. Además, las proteínas de la escalera contienen una secuencia integral Strep-tag™ II y se pueden detectar en los western blots mediante conjugados de Strep-Tactin™. La escalera de proteínas de rango alto sin teñir PageRuler se suministra lista para usar: no se requiere calentamiento, dilución ni adición de un agente reductor antes de usar.

Incluye:
• Proteínas (de 0,02 a 0,05 mg/ml de cada una) en Tris-H3PO4 de 62,5 mM (pH 7,5 a 25 °C), EDTA de 1 mM, SDS al 2 %, DTT de 100 mM, NaN3 de 1 mM, azul de bromofenol al 0,01 % y glicerol al 33 %

Aplicaciones:
• Dimensionamiento preciso de proteínas en geles de poliacrilamida-SDS y Western blots.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Para utilizar con (aplicación)Tris-acetate gels
Compatibilidad del gelGeles Bolt™ Bis-Tris Plus, Geles de tricina Novex™, geles de Tris-glicina Novex™, geles NuPAGE™ Bis-Tris, geles de Tris-acetato NuPAGE™, geles SDS-PAGE
Peso molecular250, 200, 150, 120, 100, 85, 70, 60 kDa
Línea de productosPageRuler
Tipo de productoMarcadores moleculares de proteínas
Cantidad2 × 250 ml
Listo para cargar
Aplicaciones recomendadasGeles de Tris-acetato
Condiciones de envíoAprobado para su envío en hielo húmedo o seco
Tipo de tinciónSin teñir
Tipo de sistemaWestern Blotting, SDS-PAGE
Number of Markers8
Intervalo de tamañosDe 60 a 250 kDa
Unit SizeEach
Contenido y almacenamiento
Tras su recepción a 20 °°C. Producto enviado con un paquete de hielo.

Preguntas frecuentes

The upper bands of the ladder are missing. What could be the reason?

The upper bands of the ladder may be degraded by proteases. Ladder, gel, buffer, pipettes, pipette tips, or equipment can be contaminated by proteases during usage. A general recommendation would be to avoid working with proteases in the same room. We would recommend preparing fresh solutions, cleaning the equipment, and using clean pipettes and tips. If the ladder itself is contaminated, please use a new tube of the ladder.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Some additional bands or smears were observed on the gel when using a PageRuler unstained ladder. What may have caused this?

Additional bands can appear due to dithiothreitol (DTT) oxidation in the storage buffer. Please add newly prepared DTT solution to the final concentration of 100 mM and boil for 5 min at 95 degrees C. This should solve the issue. Addition of DTT is NOT recommended for prestained protein ladders, since too high a concentration of reducing agents can cause protein destaining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do the proteins in Thermo Scientific protein ladders have a His-Tag or would otherwise react with an anti-His-Tag antibody?

No, proteins in Thermo Scientific protein ladders are not His tagged. However, non-specific interaction between the ladder proteins and primary or secondary antibodies is possible and some His-Tag detection systems, such as Thermo Scientific 6xHis Protein Tag Stain Reagent Kit, show non-specific interaction. The protein ladder bands are more readily detected when using high antibody concentrations. The non-specific cross-reactivity is difficult to predict, it often has a different pattern dependent on the antibodies used in each individual experiment. The most general way to handle this problem would be to use lower concentrations of antibodies and to use lower amount of protein ladders. It may also be useful to leave one empty well between the ladder and the sample to overcome a possible leakage of the signal to the nearby sample lane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can Thermo Scientific protein ladders be detected by Strep-Tactin conjugates?

PageRuler Unstained protein ladders can be detected directly on Western blots by using Strep-Tactin conjugates or an antibody against the Strep-tag II sequence. All PageRuler and Spectra ladder proteins contain an integral Strep-tag II sequence, however the prestained ladders cannot be detected by Strep-Tactin conjugates.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why is non-specific binding detected after Western blot?

Protein ladder bands can sometimes be detected with chemiluminescent techniques due to non-specific interactions of ladder proteins with either primary or secondary antibodies (or with both). The ladder bands are only rarely detected by chromogenic substrates. The extremely high sensitivity of the chemiluminescent assays is needed to see the bands, so the actual degree of cross-reactivity is low. The non-specific cross-reactivity is difficult to predict, it often has a different pattern depending on the antibodies used. If antibodies recognize a linear epitope, the cross-reactivity may be due to sequence homology. If antibodies react with a denaturation-resistant conformational epitope it could be impossible to identify the exact reason for detected cross-reactivity. The most general way to handle this problem would be to use lower concentrations of antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.