Fetal Bovine Serum, embryonic stem cell-qualified, New Zealand origin
Fetal Bovine Serum, embryonic stem cell-qualified, New Zealand origin
Gibco™

Fetal Bovine Serum, embryonic stem cell-qualified, New Zealand origin

Gibco™ fetal bovine sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust ofMás información
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Número de catálogoCantidad
30044333500 mL
Número de catálogo 30044333
Precio (MXN)
-
Cantidad:
500 mL

Gibco™ fetal bovine sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

  • Sera Category: Specialty
  • Origin: New Zealand
  • Endotoxin level: ≤10 EU/mL
  • Hemoglobin level: ≤25 mg/dL

Specially tested for the ability to sustain undifferentiated cellular morphology of embryonic stem cells.

Especificaciones
Concentración de endotoxinas≤10 EU/mL
Grado de pureza o calidadES Cell-Qualified
Condiciones de envíoDry Ice
EspecieCattle/Bovine
AgeFetal
País de origenNueva Zelanda
FormularioLíquido
FormatoBottle
Tipo de productoFetal Bovine Serum
Cantidad500 mL
Serum TreatmentStandard (Sterile-filtered)
EsterilidadEstéril
Unit SizeEach
Contenido y almacenamiento
Store in freezer (-5°C to -30°C).

Preguntas frecuentes

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

If my FBS arrives partially thawed, can I still use it?

Our studies have shown that short-term storage of thawed FBS at 4 degrees C for up to 28 days causes no decrease in growth or viability performance. FBS stored at 4 degrees C for longer periods of time should be evaluated on a case-by-case basis should this storage condition be necessary.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Sometimes I notice flocculence in serum. What could it be?

Flocculence may appear in FBS for a variety of reasons. The most common reason is the denaturation of serum lipoproteins. You may observe fibrin, one of the clot-forming proteins present in serum, after the serum has been thawed. This should not affect product performance. To remove the flocculence, transfer the serum to sterile tubes and centrifuge the material briefly at 400 x g. Then filter the resulting supernant along with your media. Do not attempt to filter serum containing flocculence, it may clog filters.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why does material precipitate in FBS upon storage in the refrigerator?

Gibco FBS is not pre-aged. When stored at 2 to 8 degrees C, the possibility exists for various proteins and lipoproteins in serum (e.g., cold agglutinins, fibrinogen, vitronectin, etc.) to aggregate, and form either perceptible material or observed turbidity. This should not affect serum performance. We recommend that you store FBS at -20 degrees C and avoid repeated freeze-thaw cycles.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.