Dynabeads™ M-270 Carboxylic Acid, for OEM and industrial use only
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Thermo Scientific™

Dynabeads™ M-270 Carboxylic Acid, for OEM and industrial use only

El ácido carboxílico Dynabeads™ M-270 son partículas superparamagnéticas hidrófilas de 2,8 µm de tamaño, compuestas de poliestireno altamente entrecruzado. LosMás información
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Número de catálogoCantidad
34300D10 mL
Número de catálogo 34300D
Precio (MXN)
-
Cantidad:
10 mL
El ácido carboxílico Dynabeads™ M-270 son partículas superparamagnéticas hidrófilas de 2,8 µm de tamaño, compuestas de poliestireno altamente entrecruzado. Los grupos de ácido carboxílico en la superficie permiten la formación de uniones de amida covalente a ácidos nucleicos, proteínas/péptidos y otros ligandos a través de grupos de aminas primarias o sulfhidrilos, sin necesidad de bloqueo de proteínas. Se requiere la activación a través de carbodiimida y se pueden introducir grupos reactivos alternativos mediante entrecruzadores disponibles comercialmente.

Las microesferas magnéticas Dynabeads™ se utilizan ampliamente como fase sólida para la presentación de anticuerpos o antígenos en sistemas de inmunoensayo clínico automatizados, donde la combinación de antígenos o anticuerpos específicos y las propiedades superparamagnéticas de las microesferas proporcionan cinética de reacción rápida tanto en el proceso de recubrimiento y la separación como en el lavado del analito.

El producto cumple con los altos estándares de la prestigiosa Dynal en cuanto a la reproducibilidad y la capacidad de automatización, al tiempo que mejora la fiabilidad de los ensayos.

Ventajas:

• Inmovilización eficaz y reproducible de anticuerpos (u otros ligandos)
• Manipulación fácil y reproducible en la fabricación
• Separación y lavado rápidos
• Comportamiento reproducible en automatización sin requisitos de mezcla, con procedimientos de lavado rápidos y eficientes
• Acoplamiento rápido sin necesidad de bloqueo de proteínas
• Generalmente de fondo bajo y alta relación señal-ruido

Aplicaciones
Ideales para inmunoensayos basados en partículas magnéticas que requieren una señal de fondo baja.

Nota:
Este formato de producto en particular es para clientes de gran volumen, disponible para OEM. Este producto también está disponible en volúmenes más pequeños (14306D y 14305D).

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Concentración100 mg⁄ml
Línea de productosDynabeads
Cantidad10 mL
Condiciones de envíoTemperatura ambiente
Funcionalidad de superficieÁcido carboxílico, hidrófilo
Diámetro (métrico)2,8 μm
MaterialPoliestireno
Tipo de productoGránulo de ácido carboxílico
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (2–8° C).

Preguntas frecuentes

What reactive groups do you offer for your surface-activated Dynabeads magnetic beads?

We offer tosyl, epoxy, carboxylic acid and amine activated Dynabeads magnetic beads. Please see the link (https://tools.thermofisher.com/content/sfs/brochures/Surface_Activated_Dynabeads.PDF) for a comparison of the beads.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the density of Dynabeads magnetic beads?

The density of Dynabeads magnetic beads is a challenging property to determine. The reason is that Dynabeads magnetic beads have a 17-37% magnetic iron oxide content in order to have a reasonable magnetic separation time, and the density of the iron oxide is about 4.9 g/cm3. Dynabeads magnetic beads are composite materials, being a mix of polymers and iron oxide, and there are very few polymers that have a density below 1.

The sedimentation rate depends on the bead diameter squared, so the sedimentation of a 1 µm bead is much slower than that of 2.8 µm. The effect of diameter on sedimentation rate is to some extent counteracted by the fact that smaller beads need to have a higher content of iron oxide for magnetic separation applications. Typically, our M-280 Dynabeads (diameter 2.8 µm) have a density of 1.4 g DS/cm3 (DS = dry substance), our M-270 Dynabeads (diameter 2.8 µm) and M-450 Dynabeads (diameter 4.5 µm) have a density of 1.6 g DS/cm3, and our MyOne Dynabeads (diameter 1 µm) have a density of 1.8 g DS/cm3.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center as well as our Dynabeads Cell Isolation and Expansion Support Center and Protein Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), and Pulldown Support Center.

I am using surface-activated Dynabeads magnetic beads. When do I need to use a linker secondary antibody?

A linker antibody provides proper orientation of the target-specific primary antibody. Optimal orientation of the primary antibody is more important for reacting with larger organelles than for small organelles or membrane fractions. Different linkers can be used, but we recommend using an Fc-binding antibody, such as a monoclonal or polyclonal anti-mouse IgG. The linker antibody must be affinity purified and not contain stabilizers such as sugars or proteins that may bind to the Dynabeads magnetic beads. The specific primary antibody, if polyclonal, must be affinity purified in order to provide a high density of the specific antibody on the Dynabeads magentic beads surface.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the definition of superparamagnetic, and what does this mean for my cell isolation application with Dynabeads magnetic beads?

Superparamagnetic means that the Dynabeads magnetic beads exhibit magnetic properties when placed within a magnetic field, but have no residual magnetism when removed from the magnetic field.

This means that your targeted cells, proteins, or nucleic acids are only subjected to magnetic forces during the time the beads are on the magnet. The beads do not aggregate, but remain evenly dispersed in suspension.

Find additional tips, troubleshooting help, and resources within our Dynabeads Cell Isolation and Expansion Support Center.

Are the antibodies on your Dynabeads magnetic beads for cell isolation/activation/expansion covalently bound to the beads?

Yes. The antibodies are covalently bound and should be very stable in your applications.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Dynabeads™ M-270 Carboxylic Acid, for OEM and industrial use only FAQs