Dynabeads™ SILANE Genomic DNA Kit

The biggest cause of yellowing is the addition of isopropanol when Lysis/Binding Buffer is used to make Wash Buffer 1. The use of poor quality isopropanol (not molecular grade) will cause rapid yellowing. High quality alcohols do not cause this.

Please review the following possibilities for why your Dynabeads magnetic beads are not pelleting:

- The solution is too viscous.
- The beads have formed aggregates because of protein-protein interaction.

Try these suggestions: - Increase separation time (leave tub on magnet for 2-5 minutes)
- Add DNase I to the lysate (~0.01 mg/mL)
- Increase the Tween 20 concentration to ~0.05% of the binding and/or washing buffer.
- Add up to 20 mM beta-merecaptoethanol to the binding and/or wash buffers.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

That depends. If you want to isolate intact nucleosomes (histones + DNA complex), that is not possible with Dynabeads SILANE beads. But you can isolate DNA from nucleosomes if they are not cross-linked. For chromatin immunoprecipitation, you could consider the MAGnify Chromatin Immunoprecipitation System.

The yield will depend on the number of nucleated cells present in the sample. One unit (200 µL) of Dynabeads DNA DIRECT Universal Kit will isolate at least 200 ng of high quality, PCR-ready genomic DNA per sample, sufficient for 10 PCR reactions. The size of the sample used should be adjusted to match the Dynabeads magnetic beads capacity. A typical starting sample is 10,000 cultured cells or 10 µL blood. The protocol can be adjusted to support 30 µL blood samples, sufficient for 30-50 downstream PCR amplifications (600 ng to 1 µg DNA).

For biotin-labeled DNA that is less than 1 kb, we recommend you use Dynabeads M270 Streptavidin (Cat. No. 65305) and MyOne C1 magnetic beads (Cat. No. 65001). We recommend our Dynabeads KilobaseBINDER Kit (Cat. No. 60101), which is designed to immobilize long (>1 kb) double-stranded DNA molecules. The KilobaseBINDER reagent consists of M-280 Streptavidin-coupled Dynabeads magnetic beads along with a patented immobilization activator in the binding solution to bind to long, biotinylated DNA molecules for isolation. Please see the following link (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/napamisc/capture-of-biotinylated-targets/immobilisation-of-long-biotinylated-dna-fragments.html) for more information in regards to long biotinylated DNA fragment isolation.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.