Medio L-15 de Leibovitz, polvo
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Gibco™

Medio L-15 de Leibovitz, polvo

Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue.Más información
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Número de catálogoCantidad
4130007010 L
4130003910 x1 L
Número de catálogo 41300070
Precio (MXN)
-
Cantidad:
10 L
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Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO2 equilibration.

This L-15 is modified as follows:

With: Galactose, Phenol Red, L-glutamine, Sodium Pyruvate

Without: Glucose, HEPES, Sodium Bicarbonate

The complete formulation is available.

L-15 contains no proteins, lipids, or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). Powder forms of Gibco™ L-15 cell culture medium require pH adjustment and filtration at the time of preparation (see protocol for details).

Please note that the shelf life on delivered products varies. This product has a minimum shelf life of 6 months at time of shipment. For more information regarding product shelf life, including details on specific product lots, please contact Customer Service.

no está indicado para uso terapéutico en humanos ni en animales. Los usos que vayan más allá del uso previsto podrían infringir la legislación local. Para uso diagnóstico in vitro.

Especificaciones
Línea de célulasCélulas renales de mono HEP-2
Tipo de célulaExplantes de tejido humano embrionario y adulto
Calidad de fabricacióncGMP-compliant under the ISO 13485 standard
Línea de productosGibco
Tipo de productoMedio L-15 de Leibovitz
Cantidad10 L
Duración de almacenamiento36 meses a partir de la fecha de fabricación
Condiciones de envíoTemperatura ambiente
ClasificaciónOrigen animal
FormularioPolvo
EsterilidadEstéril con filtro
Con aditivosGlutamina, Rojo de fenol, Piruvato sódico, Galactosa
Sin aditivosSin glucosa, Sin HEPES, Sin bicarbonato sódico
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: 2-8 °C.
Condiciones de envío: Ambiente
Vida útil: 36 meses a partir de la fecha de fabricación

Preguntas frecuentes

What is the osmolality of Leibovitz's L-15 Medium?

We do provide osmolality information on the certificate of analysis. All lots of Leibovitz's L-15 Medium will meet the osmolality specification of 300-340 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the Leibovitz media you offer?

We offer the following Leibovitz media:

- Leibovitz's L-15 Medium, powder: Cat. Nos. 41300039 (10x 1L) and 41300070 (1x 10L)
- Leibovitz's L-15 Medium, no phenol red: Cat. No 21083027 (1x 500 mL)
- Leibovitz's L-15 Medium: Cat. Nos.11415064 (1x 500 mL) and 11415114 (10x 500 mL) (for sale in N. America, Latin America, and Asia-Pacific regions; Cat. No. 11415049 (for sale in Europe, Africa, and the Middle East
- Leibovitz's L-15 Medium with GlutaMAX Supplement: Cat. No. 31415029 (1x 500 mL)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of my powdered media once I reconstitute it?

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

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