Matrix Standards Kit, BigDye™ Terminator v3.1, for 31xx/3500/SeqStudio™/SeqStudio™ Flex
Matrix Standards Kit, BigDye™ Terminator v3.1, for 31xx/3500/SeqStudio™/SeqStudio™ Flex
Applied Biosystems™

Matrix Standards Kit, BigDye™ Terminator v3.1, for 31xx/3500/SeqStudio™/SeqStudio™ Flex

Este kit contiene dos tubos de patrón utilizados para generar un espectro necesario al analizar fragmentos de ADN etiquetados deMás información
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Número de catálogoCantidad
43369741 kit
Número de catálogo 4336974
Precio (MXN)
-
Cantidad:
1 kit
Este kit contiene dos tubos de patrón utilizados para generar un espectro necesario al analizar fragmentos de ADN etiquetados de BigDye Terminator v3.1 en los analizadores genéticos de las series 31xx, 3500, SeqStudio y SeqStudio Flex.Cada tubo contiene una mezcla de cuatro colores diferentes de fragmentos de ADN etiquetados con colorante fluorescente suspendidos en el tampón de TE 1X.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Para utilizar con (aplicación)Secuenciación
Para utilizar con (equipo)Analizadores genéticos de la serie 31xx, analizador genético SeqStudio™, analizadores genéticos de la serie 3500, analizadores genéticos de la serie SeqStudio Flex
Etiqueta o tinteROX, TAMRA™, rodamina 110, rodamina 6G
Línea de productosBigDye Terminador, BigDye
Tipo de productoPatrón de matriz
Cantidad1 kit
Condiciones de envíoHielo húmedo
FormatoKit
Unit SizeEach
Contenido y almacenamiento
Almacenar a entre 2 y 8 °C, protegido de la luz. No la congele.

Preguntas frecuentes

When I started my run on the Applied Biosystems 3500/3500xL Genetic Analyzer, an error occurred: "Unstable Electrophoresis current detected, check for air bubbles" but I don't see any bubbles. What else can cause this?

Some of the other causes of an Unstable Electrophoresis current detected error message are:

1.Leak on the system
2. Polymer that:
-Has expired
-Has been left on the instrument for more than the recommended time
-Is a mixture of expired polymer and non-expired polymer
3. Running Buffer that was:
-On the instrument longer than 2 weeks
-Not filled to the fill line or evaporated below the fill line
4. An arcing event that was not cleaned afterwards using the water wash wizard
5. Not performing regular maintenance on the instrument
6. Hardware issues

Inspect the system for leaks. If you do not see any leaks on the system, perform the wash the pump chamber and channels wizard using the conditioning pouch and place fresh, non-expired polymer and fresh Anode and Cathode buffer containers. If the problem persists, a service call may be required.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

Do I need to replace the buffer every 14 days if the Applied Biosystems 3500/3500xL Genetic Analyzer is not in use?

If the instrument is not in use, it is not necessary to replace the buffer every 14 days. However, the buffer level needs to remain at the fill line to prevent the capillary or array from drying out. Top off the buffer volume with water if necessary. Replace the anode and cathode buffer containers prior to starting a new run.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

How do I make a spectral standard for the Applied Biosystems 3130 series Genetic Analyzer?

The spectral calibration on the Applied Biosystems 3130 series systems can be performed by either using a Matrix Standard or a Sequencing Standard. The Matrix Standards will give 4 peaks, each of a different color whereas the Sequencing Standard is pre-sequenced and cleaned DNA that is dried down and just needs to be resuspended.

To make up the Matrix Standard:

  1. Vortex the Matrix Standard tube for 1 min at full speed and pulse spin in a microcentrifuge.
  2. Dilute the Matrix Standard in Hi-Di Formamide in a 1.5 mL microcentrifuge tube according to the following dilutions:
      For 4 capillary arrays
    • 36 cm and 80 cm arrays: mix 2.5 µL of Matrix Standard with 47.5 µL of Hi-Di Formamide.
    • 50 cm array: mix 2.5 µL of Matrix Standard with 97.5 µL of Hi-Di Formamide.

      For 16 capillary arrays
    • 36 cm and 80 cm arrays: mix 10 µL of Matrix Standard with 190 µL of Hi-Di Formamide.
    • 50 cm array: mix 5 µL of Matrix Standard with 195 µL of Hi-Di Formamide.
  3. Mix thoroughly and pulse spin in a microcentrifuge.
  4. Denature at 95 degrees C for 2 min and dispense 10 µL of reagent into wells A1–H12 (16 capillaries) or A1–D1 for 4 capillaries on a 96-well plate.
  5. Briefly spin to ensure there are no air bubbles at the bottom of the well.
  6. Set up and run the spectral calibration as per the Applied Biosystems 3130/3130xl Genetic Analyzers Getting Started Guide


More information on making up the Matrix Standards can be found in the BigDye Terminator v.3.1 Matrix Standard Kit for 3130 and 3100 Series Systems Product Insert or the BigDye Terminator v.1.1 Matrix Standard (Dye Set E) for 3100, 3130, and 3500 Series Genetic Analyzers Product Insert

To make up the Sequencing Standard:
  1. Resuspend one tube of the Sequencing Standard with 170 µl of Hi-Di Formamide.
  2. Vortex at full speed for 1 min and pulse spin in a microcentrifuge.
  3. Denature at 95 degrees C for 2 min and dispense 10 µL of reagent into wells A1–H12 (16 capillaries) or A1–D1 for 4 capillaries on a 96-well plate.
  4. Briefly spin to ensure there are no air bubbles at the bottom of the well.
  5. Set up and run the spectral calibration as per the Applied Biosystems 3130/3130xl Genetic Analyzers Getting Started Guide


Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.