Applied Biosystems™

BigDye™ Terminator Plus with M13 HiRes Primers

Name: BigDye™ Terminator Plus with M13 HiRes Primers
SKU: 4337469

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Número de catálogo	Cantidad
4337469	192 μL
4337470	800 μL
4337471	8 mL
4337472	40 mL
4337473	200 mL

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Número de catálogo 4337469

Precio (MXN)

Cantidad:

192 μL

The BigDye™ Terminator BDT Plus Cycle Sequencing Kit with HI-Res Primers is a Sanger sequencing solution designed to enable faster results, improved performance, and enhanced read quality across a broad range of sample types. Combining a newly developed high-processivity enzyme with optimized HI-Res primers, this integrated solution enables improved sequencing accuracy—including enhanced 5' (five-prime) end resolution—while maintaining full compatibility with standard BigDye™ workflows using POP-7™ polymer. Compared to legacy BigDye™ Terminator v3.1 chemistry, the kit helps provide greater speed, flexibility, and data quality without requiring workflow changes.

Key Features and Benefits

Accelerate sequencing workflows with up to ~50% faster time to results
Increase success rates and reduce re-runs for greater laboratory efficiency
Improve performance on challenging templates using a high-processivity enzyme and optimized primers
Enhance 5' end resolution within standard workflows using POP-7™ polymer
Reduces the need for dedicated workflows or alternative polymers such as POP-6™
Maintain existing workflows with full compatibility across BigDye™ protocols and CE instruments

Product Details

The BDT Plus kit with HI-Res primers integrates advanced enzyme chemistry with optimized primer design to improve sequencing performance from template to readout. The high-processivity enzyme enhances incorporation efficiency of dye-labeled terminators, enabling stronger signal intensity and more consistent sequencing results across a wide range of sample types, including GC-rich regions and structurally complex templates.

The inclusion of HI-Res primers further improves sequencing quality by enhancing signal resolution, particularly at the 5' end of reads. This region has historically required modified workflows using alternative polymers such as POP-6™ to achieve improved resolution. In contrast, the combined BDT Plus chemistry and HI-Res primers enable high-quality 5' end data within standard workflows using POP-7™ polymer, simplifying operations and reducing the need for additional method development.

Designed as a ready-to-use solution, the kit functions as a drop-in replacement for existing BigDye™ Terminator chemistries. Laboratories can adopt the kit without changing established protocols, instruments, plastics, or cleanup methods. This compatibility enables users to benefit from improved sequencing performance while maintaining workflow continuity.

The kit supports a wide range of Sanger sequencing research applications, including assay development, mutation detection, plasmid verification, and confirmatory sequencing. It is particularly well suited for laboratories working with diverse or challenging sample types, where consistent performance, high data quality, and workflow efficiency are critical.

By enabling faster turnaround times, improved sequencing performance, and enhanced 5' end resolution, the BDT Plus kit with HI-Res primers helps laboratories increase productivity, reduce re-runs, and generate high-confidence sequencing data.

For Research Use Only.

Especificaciones

Para utilizar con (aplicación)Sanger Sequencing, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing

Para utilizar con (equipo)SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers

Línea de productosBigDye Terminator

Tipo de productoSequencing Kit

Cantidad192 μL

TécnicaFluorescent Dye Terminator Sequencing

Compatibilidad de plantillasBAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15 Kb), PCR Amplicons

FormatoTube

Isolation TechnologyMagnetic Bead

Sequencing TypeGenome and DNA Sequencing

Unit SizeEach

Preguntas frecuentes

Can I dilute sequencing reactions when using BigDye Terminator Plus Cycle Sequencing Kit?

Yes, sequencing reactions can be diluted when using the BigDye Terminator Plus Cycle Sequencing Kit by using the 5X Sequencing Buffer. When reducing the Ready Reaction Mix volume, additional buffer should be added to maintain a final 1X buffer concentration.
The 5X Sequencing Buffer contains buffer components (for example, Mg²⁺ and Tris) at a higher concentration than the Ready Reaction Mix.
Specifically, the Ready Reaction Mix provides buffer at 2.5X, while the Sequencing Buffer is at 5X, meaning the buffer components are twice as concentrated in the 5X Sequencing Buffer compared to the Ready Reaction Mix. To maintain a final 1X buffer concentration in the sequencing reaction when reducing the volume of Ready Reaction Mix, the volume of 5X Sequencing Buffer added should compensate for the missing buffer. This can be calculated as:

Volume of 5X Sequencing Buffer = ½ × (Nominal Ready Reaction Mix volume – Actual Ready Reaction Mix volume)

Example: For a 10 µL sequencing reaction:
Nominal Ready Reaction Mix volume (full reaction): 4 µL
Actual Ready Reaction Mix volume used: 1 µL
Volume of 5X Buffer = ½ × (4 µL – 1 µL)
Volume of 5X Buffer = ½ × 3 µL = 1.5 µL
In this example, adding 1.5 µL of 5X Sequencing Buffer compensates for the reduced Ready Reaction Mix volume and maintains the correct final buffer concentration for optimal sequencing performance.
This helps to ensure that the overall buffer composition remains consistent despite dilution of the sequencing chemistry.

Can BigDye Terminator Plus Cycle Sequencing Kit be used with dGTP chemistry?

Yes, BigDye Terminator Plus Cycle Sequencing Kit can be used with dGTP chemistry when sequencing challenging templates. When using dGTP, standard reaction setups can be followed, with the addition of a small proportion of dGTP BigDye master mix to the sequencing reaction. Optimization may be required depending on the template.

Is BigDye Terminator Plus Cycle Sequencing Kit compatible with sequencing additives such as DMSO or betaine?

Yes, BigDye Terminator Plus Cycle Sequencing Kit is compatible with commonly used sequencing additives, including GC enhancers, DMSO, betaine, sulfolane, and sequencing enhancer solutions. These additives may be used when working with particularly challenging templates.

How does BigDye Terminator Plus Cycle Sequencing Kit perform on challenging templates such as GC-rich regions?

BigDye Terminator Plus Cycle Sequencing Kit is designed to support sequencing performance across challenging templates, including GC-rich regions, repetitive sequences, and templates with secondary structure.

How does BigDye Terminator Plus Cycle Sequencing Kit compare to BigDye Terminator v3.1 Cycle Sequencing Kit in read length and performance?

BigDye Terminator Plus Cycle Sequencing Kit is designed to support longer read lengths and more consistent signal across challenging templates compared to BigDye Terminator v3.1 Cycle Sequencing Kit, particularly in GC-rich or structured regions.

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