Kit ARNasa P de verificación de instrumentos de matriz de baja densidad 7900HT TaqMan™

Applied Biosystems™

Kit ARNasa P de verificación de instrumentos de matriz de baja densidad 7900HT TaqMan™

The 7900HT TaqMan® Low Density Array Instrument Verification RNase P kit is used to validate the performance of the AppliedMás información

Número de catálogo	Cantidad
4351468	1 kit

Número de catálogo 4351468

Precio (MXN)

Cantidad:

1 kit

The 7900HT TaqMan® Low Density Array Instrument Verification RNase P kit is used to validate the performance of the Applied Biosystems® 7900HT Fast Real-Time PCR Systems with the TaqMan® Low Density Array Block Upgrade. The 7900HT TaqMan® Low Density Array Instrument Verification RNase P kit contains an empty TaqMan® Low Density Array and 8 vials of RNase P detection reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each vial contains premixed reagents (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. The 7900HT TaqMan® Low Density Array Instrument Verification RNase P kit must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tinte calibradoFAM

Tipo de calibraciónCalibración ARNasa P, placa de verificación de instrumentos

Método de detecciónFluorescence

Para utilizar con (aplicación)PCR en tiempo real (PCRq)

Para utilizar con (equipo)Sistema LDA 7900 HT

Línea de productosTaqMan

Cantidad1 kit

Tipo de productoInstrument Verification Kit

Unit SizeEach

Contenido y almacenamiento

Contains an empty TaqMan™ Low Density Array and 8 vials of RNase P detection reagents. Store at -15 to 25°C. Protect from light

Preguntas frecuentes

When do I need to calibrate the 7900HT Fast Real-Time PCR System?

Recommended Maintenance Schedule

Power on/off the computer controlling the instrument: Weekly
Check computer disk space. If necessary, archive or back up your experiment files and instrument settings: Weekly
Run disk cleanup and disk defragmentation: Every month
Background calibration: Every month
Perform an instrument self test: Every month
Pure dye calibrations: Every 6 months
RNaseP instrument verification: After the instrument has been moved, or as needed to verify instrument performance

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I use my 7900HT System RNase P instrument verification plates with both the 7900HT System and the QuantStudio 6 and 7 Flex Real-Time PCR Systems?

Only the TaqMan RNase P Instrument Verification Plate, Fast 96-well (for 0.1 mL block) (Cat. No. 4351979) is compatible with both the 7900HT System and the QuantStudio 6 and 7 Flex Real-Time PCR Systems. The following RNase P instrument verification plates can be used on the 7900HT System, but not on the QuantStudio 6 and 7 Flex Real-Time PCR Systems:
• TaqMan RNase P Instrument Verification Plate, 96-well (Cat. No. 4310982) (for 96-well standard block)
• TaqMan RNase P Instrument Verification Plate for 7900HT System, 384-well (Cat. No. 4323306) (for 384-well block)
• 7900HT TaqMan Low Density Array Instrument Verification RNase P Kit (Cat. No. 4351468) (for TaqMan Array Card block)

The following RNase P instrument verification plates can be used on the QuantStudio 6 and 7 Flex Real-Time PCR Systems, but not on the 7900HT System:
• TaqMan RNase P Instrument Verification Plate for ViiA7 System, 96-well (Cat. No. 4432382) (for 96-well standard block)
• TaqMan RNase P Instrument Verification Plate for ViiA7 System, 384-well (Cat. No. 4455280) (for 384-well block)
• ViiA7 Array Card RNase P Verification Kit (Cat. No. 4432265) (for TaqMan Array Card block)

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What is the difference between a Fast 96-well and standard 96-well block?

There are two main differences between a Fast 96-well and standard 96-well block for the Applied Biosystems real-time PCR systems:
• Reaction well volume: The Fast 96-well blocks have 0.1 mL reaction wells and are compatible with 0.1 mL plates and tubes. The standard 96-well blocks have 0.2 mL reaction wells and are compatible with 0.2 mL plates and tubes.
• Ramp rate: The fast 96-well blocks have a higher maximum ramp rate than standard blocks for the same real-time PCR system. However, both the fast 96-well and standard 96-well blocks are capable of running standard and fast chemistry.

Note: For newer instruments (QuantStudio 3 and 5 Real-Time PCR Systems and later) "0.1 mL" and "0.2 mL" are being used in place of "fast" and "standard" as designations for the two 96-well block formats.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.