Reactivos de RT-PCR de un paso AgPath-ID™ con manual
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Applied Biosystems™

Reactivos de RT-PCR de un paso AgPath-ID™ con manual

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverseMás información
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Número de catálogoN.º de reacciones
4387424M500 reacciones
4387391M1000 reacciones
AM1005M100 reacciones
Número de catálogo 4387424M
Precio (MXN)
-
N.º de reacciones:
500 reacciones

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
• Recommended for RNA pathogen amplification
• Consistent amplification of RNA targets with high specificity and sensitivity
• Optimized to work with your target-specific primers and probes
• Contains ROX for quantitative fluorescent signal normalization

AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
DescripciónFunciona en todas las plataformas AB, pero está optimizado para la 7500.
Fidelidad (frente a Taq)25 X
Para utilizar con (equipo)Sistema 7500
FormatoTubo
N.º de reacciones500 reacciones
Colorante de referencia pasivaROX (premezclado)
PolimerasaADN polimerasa AmpliTaq Gold
Línea de productosAgPath-ID, Ambion
Tipo de productoOne-Step RT-PCR Reagent
Cantidad500 reacciones
Transcriptasa inversaArrayScript™
Suficiente para500 reacciones
Volumen7 mL
Concentración25X
Método de detecciónSonda de cebado
Para utilizar con (aplicación)Animal Health, Pathogen Detection
GC-Rich PCR PerformanceHigh
Método de PCR1-step RT-qPCR
Velocidad de reacciónEstándar
Unit SizeEach
Contenido y almacenamiento
Suficiente para 500 reacciones de 25 µl, contiene:

• 7 ml 2 x tampón de RT-PCR

• 550 µl 25 x mezcla de enzimas para RT-PCR

• 25 ml de agua libre de nucleasas.

Preguntas frecuentes

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.