Megaplex™ PreAmp Primers, Human Pool A v2.1
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Applied Biosystems™

Megaplex™ PreAmp Primers, Human Pool A v2.1

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Para su uso cuando la sensibilidad del ensayo es de la máxima importancia, los cebadores Megaplex™ PreAmp mejoran significativamente laMás información
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Número de catálogoCantidad
439923350 reacciones
Número de catálogo 4399233
Precio (MXN)
-
Cantidad:
50 reacciones
Para su uso cuando la sensibilidad del ensayo es de la máxima importancia, los cebadores Megaplex™ PreAmp mejoran significativamente la capacidad de detectar microARN de baja expresión, lo que permite generar un perfil de expresión completo utilizando mucha menos muestra, tan solo 1 ng del ARN total de entrada.Con contenido adaptado a los cebadores Megaplex™ RT, los cebadores Megaplex™ PreAmp, grupo humano A v2.1 se ha diseñado para utilizarse con cebadores Megaplex™ RT, grupo humano A v2.1.Después de la transcripción inversa utilizando los cebadores Megaplex™ RT, los cebadores Megaplex™ PreAmp junto con la mezcla maestra TaqMan® PreAmp amplifican uniformemente todos los microARN antes de la cuantificación mediante PCR en tiempo real utilizando las tarjetas de MicroARN de matriz TaqMan®.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Características ecológicasEmbalaje sostenible
N.º de reacciones50 reacciones
CebadorCebadores PreAmp
Línea de productosMegaplex, TaqMan
Tipo de productoCebador PreAmp
Cantidad50 reacciones
Condiciones de envíoTemperatura ambiente
EspecieHumano
Suficiente para50 reacciones
GC-Rich PCR PerformanceBajo
Método de PCRqPCR
Unit SizeEach
Contenido y almacenamiento
Almacenar a -20 °C.

Preguntas frecuentes

I want to purchase TaqMan Array Human MicroRNA A Cards v2.0 (Cat. No. 4398965). What other reagents do I need to purchase in addition to these cards?

In order to perform reverse transcription on these cards, you will need the following:

  • TaqMan MicroRNA Reverse Transcription Kit (Cat. No. 4366596)
  • Megaplex RT Primers pools (for human Type A cards Cat. No. 4399966, for Type B cards Cat. No. 4444281)
You can proceed with or without a preamplification step which depends on the total RNA amount. Total RNA amount of 1‐1000 ng supports reverse transcription reaction with preamplification, whereas 350‐1000 ng of total RNA supports a reaction without preamplification. In case you are performing preamplification you would also need the TaqMan PreAmp Master Mix (Cat. No. 4391128) and the Megaplex PreAmp Primers, Human Pool A v2.1 (Cat. No. 4399233). You will also need the Master Mix for the qPCR reaction. It is not included in the card kit. The recommended Master Mix is: TaqMan Fast Advanced Master Mix – run mode Fast (Cat. No. 4444556) For further information, please see the following link.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

View all Megaplex™ PreAmp Primers, Human Pool A v2.1 FAQs