PrepSEQ™ Nucleic Acid Extraction Kit for Food and Environmental Testing
PrepSEQ™ Nucleic Acid Extraction Kit for Food and Environmental Testing
Applied Biosystems™

PrepSEQ™ Nucleic Acid Extraction Kit for Food and Environmental Testing

The PrepSEQ Nucleic Acid Extraction Kit produces high-quality bacterial DNA and viral RNA samples for PCR-based detection from a wideMás información
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Número de catálogoN.º de reacciones
4428176300 preparaciones
4480466100 preparaciones
Número de catálogo 4428176
Precio (MXN)
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N.º de reacciones:
300 preparaciones
The PrepSEQ Nucleic Acid Extraction Kit produces high-quality bacterial DNA and viral RNA samples for PCR-based detection from a wide range of food and environmental samples. The quantity and quality of the nucleic acid can greatly impact the success of the sample analysis process and the overall quality of the final result. Food and environmental samples can be challenging to process because they often contain PCR inhibitors. For laboratories looking to achieve better real-time PCR results and improve overall efficiency, the PrepSEQ Nucleic Acid Extraction Kit meets these needs.

Automated Sample Preparation for PCR-Based Assays
• Extract high-quality bacterial DNA and viral RNA
• Process a wide range of food and environmental samples
• Fast time-to-results
• Prepare hundreds of samples per shift per instrument

High-Throughput Food and Environmental Sample Preparation
PCR-based bacterial assays have become an important tool in food safety and environmental testing. The Applied Biosystems PrepSEQ Nucleic Acid Extraction Kit prepares high-quality bacterial DNA and viral RNA from food and environmental samples for PCR-based testing using magnetic bead-based extraction. In combination with the automated MagMAX Express-96 Deep Well Magnetic Particle Processor or the KingFisher Flex Purification System with KingFisher 96 Deep-well Head, this optimized solution enables laboratories to prepare a wide range of sample types at rates of up to hundreds of samples per eight-hour shift.

Streamlined Workflow
An easy-to-use, easy-to-learn protocol simplifies integration of the PrepSEQ Nucleic Acid Extraction Kit into the laboratory workflow. The same protocol can be used on a wide range of sample types, and the MagMAX Express-96 instrument or the KingFisher Flex Purification System with KingFisher 96 Deep-well Head automates many of the workflow steps to reduce hands-on time.

A Complete Solution
The PrepSEQ Nucleic Acid Extraction Kit is part of a fully integrated, start-to-finish solution for food and environmental testing. The kit has been performance-verified for food and environmental testing with MicroSEQ Microbial Detection Kits, the Applied Biosystems 7500 Fast Real-Time PCR System, and RapidFinder Express Software.
Especificaciones
Tipo de producto finalTotal RNA (Bacterial), Bacterial DNA, Viral RNA
Para utilizar con (aplicación)PCR en tiempo real cuantitativa (qPCR), PCR
Para utilizar con (equipo)MagMAX™ Express-96 Deep Well Magnetic Particle Processor
Compatibilidad de alto rendimientoHigh-throughput Compatible, Automated Protocols
N.º de reacciones300 preparaciones
Línea de productosPrepSEQ
Cantidad300 preps
Tipo de muestraSwab, Bacteria (Gram +/-), Bacterial Culture, Food, Serum, Water
Cantidad de material de partida1 mL culture
ObjetivoGenomic DNA, Viral RNA
Formato96-well Plate
Isolation TechnologyEnzymatic, Magnetic Bead
Unit SizeEach
Contenido y almacenamiento
This kit contains:
• 6 bottles of Lysis Buffer
• 6 tubes of Magnetic Particles (store at 2 to 8°C)
• 3 empty bottles for binding solution
• 6 bottles of Wash Buffer Concentrate
• 3 bottles of Elution Buffer
• 3 bottles of Proteinase K Buffer
• 3 tubes of Proteinase K (store at -20°C).

Unless otherwise indicated, each component should be stored at RT.

This kit contains enough supplies for 100 extractions.

Preguntas frecuentes

I am using the PrepSEQ Nucleic Acid Extraction Kit for Food and Environmental Testing. I am finding it very difficult to resuspend the bacterial pellet. Can you offer some tips?

This is due to the pellet being too hard. We recommend ensuring maximum resuspension of the pellet in the Lysis Buffer or Proteinase K Buffer before proceeding. Also, we recommend transferring the entire contents, including the incompletely resuspended pellet (if any), to the Lysis Plate.