3500 Genetic Analyzer for Sequence Typing & Fragment Analysis
3500 Genetic Analyzer for Sequence Typing & Fragment Analysis
Applied Biosystems™

3500 Genetic Analyzer for Sequence Typing & Fragment Analysis

This 8-capillary Genetic Analyzer is specifically configured with software for both fragment analysis and sequence typing applications.We set a newMás información
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Número de catálogo 4440470
Precio (MXN)
-
This 8-capillary Genetic Analyzer is specifically configured with software for both fragment analysis and sequence typing applications.

We set a new standard in capillary electrophoresis with the 8-capillary 3500 Genetic Analyzer. These instruments are specifically designed to support the demanding performance needs of validated and process controlled environments, while retaining the unsurpassed application versatility that life science researchers expect from us.

• An 8 capillary system that can easily be upgraded to a 24-capillary system when you’re ready.
• New single-line 505 nm, solid-state long-life laser – utilizes a standard power supply; requires no heat-removal ducting.
• Powerful, integrated data collection and primary analysis software provides real-time assessment of data quality.
• Radio Frequency Identification (RFID) technology tracks key consumables data and records administrative information.
• Advanced multiplexing capabilities for DNA fragment analysis with up to six unique dyes.
• Unrivaled application flexibility – one array and one polymer are used for most applications.
• Simple setup, operation and maintenance – the easiest-to-run, easiest-to-own DNA sequencer to date.

Accurate Reliable Data Quality
The 3500 series systems significantly improves signal uniformity from instrument-to-instrument, run-to-run, and capillary-to-capillary. With a combination of intelligent hardware, powerful new algorithm and reagent combinations, these methods provide major reduction in the range of signal peak heights obtained across multiple 3500 series instruments (see figure 1).

Improved Sizing Precision
New thermal sub-system design gives enhanced performance in demanding fragment applications enabling improved sizing precision versus previous generations of capillary electrophoresis platforms (see figure 2).

Ready to Use Consumables With RFID Tags
The 3500 series introduces ready-to-use consumables with pre-packaged polymer pouches, cathode and anode buffer containers and easy-to-install capillary arrays. Each of these consumables is integrated with RFID tags that enable viewing, tracking and reporting of critical information about reagents and consumables including usage, lot number, part number, expiry date and on-instrument lifetime within the 3500 Series Data Collection Software (see figures 3 and 4).

Easy to Use New Data Collection Software
3500 Series Data Collection software breaks new ground in user-friendly navigation with an intuitive dashboard design, highly visible buttons for common operations, easy-to-read graphical displays to monitor the state of consumables and handy maintenance scheduling calendar functionality. New functionality includes simplified plate set-up, built-in primary analysis with quality control, so you can make decisions about the quality of data as it is produced on the instrument without the need to transfer output files into secondary analysis software packages (see figures 4 & 5).

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Amperaje15 A
Longitud del capilar50 cm, 36 cm
Requisitos eléctricos100 V to 240 V, 50/60 HZ, 15 A
Ordenador externoHard Drive: 2x 500GB SATA 3.0 Gb/s and 8 MB Data Burst Cache, Memory: 16 GB (2x 8GB) 1600 MHz DDR3 Non-ECC. 4th Gen Intel Core I7 Processor- 3.1 GHz Turbo Processor, Operating System: Windows 7 SP1
Para utilizar con (equipo)3500 Genetic Analyzer Capillary Array (50 cm), POP-6™ Polymer for 3500⁄3500xL Genetic Analyzers, POP-4™ Polymer for 3500⁄3500xL Genetic Analyzers, 3500 Genetic Analyzer Capillary Array (36 cm), POP-7™ Polymer for 3500⁄3500xL Genetic Analyzers
Formato96-well Plate, 8-tube Strip
Altura exterior (imperial)28.34 in.
Altura exterior (métrico)72 cm
Longitud (imperial) exterior24.01 in.
Longitud (métrico) exterior61 cm
Tensión máx.Electrophoresis Voltage - Up to 20 kV
Número de capilares8 Capillaries
Entorno operativoHumidity: 20%-80% (non-condensing), Room temp: <2° fluctuation during runs, Temperature: 15°C-30°C
Sistema operativoWindows 7 (Service Pack 1)
PolímeroPOP-4™, POP-6™, POP-7™
Consumo de polímerosReplace at earliest of 7 days⁄384 samples⁄60 Injections (384-sample pouches), Replace at earliest of 7 days⁄960 samples⁄120 Injections (960-sample pouches)
Cantidad1 sistema
Longitud de lectura≥850 bp using a 50 cm capillary and POP-7™
Compatibilidad del softwareSequencing Analysis Software, Variant Reporter™ Software, GeneMapper™ Software
Capacidad de procesamientoUp to 424 samples per day (using run module HID36 POP-7)
Garantía1 Year Limited Warranty on Parts and Labor, Service Installation and Application Training Included
Peso82 kg (approximately)
Anchura exterior (imperial)24.01 in.
Anchura exterior (métrico)61 cm
Software TypeData Collection Software, Fragment Analysis
Material de partidaADN
Unit SizeEach
Componentes del envase
4361142
Número de catálogo
4361142
Tamaño de la unidad
Cant.
1
3500 Genetic Analyzer
Número de catálogo
4405673
Tamaño de la unidad
1 system
Cant.
1
4405778
Número de catálogo
4405778
Tamaño de la unidad
Cant.
1

Preguntas frecuentes

How can I set up auto-analysis for data in GeneMapper with the 3500 Data Collection Software version 3.x?

Beginning with 3500 Data Collection Software version 3, the 3500 Data Collection Software does not support automatic secondary analysis. This is also indicated in the software release notes. To perform secondary data analysis, you will need to manually import the .fsa file into the GeneMapper software. For optimal performance, we recommend that you install the GeneMapper Software on a stand-alone computer that is not part of the 3500 Genetic Analyzer system.

Find additional tips, troubleshooting help, and resources within our Fragment Analysis Software Support Center.

I am using the 3500 Genetic Analyzer for Sequence Typing & Fragment Analysis. How can I resolve the error report "Failed to save plate. Reason - java.lang.reflect.InvocatiomTargetException: Java heap space"?

This error may occur due to a problem with the AuditSummary.aud file. The file can be renamed, forcing the data collection software to create a new one. To do this please follow the following instructions:

1. Close the Data Collection Software and exit the Integration Service (right click on the green tick in the lower right corner of the screen and select "Exit").
2. Go to D:/Applied Biosystems/3500/datastore/security and rename the Auditsummary.aud file (e.g. Auditsummary1.aud). Save the file in case you need to troubleshoot further problems.
3. Restart the computer and the instrument, then start the Data Collection Software. The Auditsummary file will be recreated automatically.
4. Turn on the computer and press Ctrl + Alt + Delete but do not log on to the computer.
5. Turn on the instrument and wait for the green light to turn on.
6. Log on to the computer and wait for the green tick in the lower right corner indicating that the Integration Service has been fully started.
7. Start the Data Collection Software.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

Is the Plate Septa, 96 well (Cat. No. 4315933) compatible with 3500/3500xL Genetic Analyzers?

Yes, it is compatible with all versions of 3500/3500xL Genetic Analyzers as well as the 3500/3500xL Dx Genetic Analyzers, specifically in RUO mode.

Is there a way to automatically upload my data into the Thermo Fisher Cloud app directly from the Applied Biosystems 3500 Genetic Analyzer, instead of using Data Collection Software?

If you are using the most recent version of Data Collection Software (v3.3), there is a way to automatically upload your data into the Thermo Fisher Cloud app from the instrument. When a run is complete, the data is stored in the results group folder that is specified in the plate record. The data will be automatically uploaded to your account if your User ID is the active ID. Results are saved in your account folders named by the plate and injections, not in the results group folder. Please go to page 43 of the 3500/3500xL Genetic Analyzer User Guide for Data Collection Software v3.3 (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/100079380_3500v3_3RUO_UG.pdf) for details regarding the Thermo Fisher Cloud app.

If you are using Data Collection Software v3.1, you would have to manually upload your data into the Thermo Fisher Cloud app from your data folder on the computer.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.