Path-ID™ Multiplex One-Step RT-PCR Kit
Path-ID™ Multiplex One-Step RT-PCR Kit
Applied Biosystems™

Path-ID™ Multiplex One-Step RT-PCR Kit

The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR). It is optimized for theMás información
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Número de catálogoN.º de reacciones
4442136500 reacciones
4442135100 reacciones
44421371000 reacciones
Número de catálogo 4442136
Precio (MXN)
-
N.º de reacciones:
500 reacciones
The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR). It is optimized for the amplification of up to four targets simultaneously using your RNA samples and TaqMan™ primer-probe sets. Reactions are run using a single-tube, one-step procedure to reverse-transcribe the RNA and amplify your targets. The kit contains a Multiplex Enzyme Mix with Arrayscript™ reverse transcriptase and AmpliTaq Gold™ DNA Polymerase, and a buffer mix.
Especificaciones
Para utilizar con (equipo)7500 Fast System, 7500 System
FormatoTubo
Inicio en calienteBuilt-In Hot Start
N.º de reacciones500 reacciones
Colorante de referencia pasivaROX (premezclado)
PolimerasaADN polimerasa AmpliTaq Gold
Línea de productosPath-ID
Tipo de productoMultiplex One-Step RT-PCR Kit
Cantidad500 reacciones
Transcriptasa inversaArrayScript™ UP
Tipo de muestraARN
Suficiente para500 reacciones
Método de detecciónSonda de cebado
Para utilizar con (aplicación)Pathogen Detection, Virus Detection
GC-Rich PCR PerformanceLow
Método de PCR1-step RT-qPCR, Multiplex qPCR
Velocidad de reacciónEstándar
Unit SizeEach
Contenido y almacenamiento
Store at -20°C. Includes 500 reactions with the following components: Multiplex RT-PCR Buffer, Multiplex Enzyme Mix, and Nuclease-free water.

Preguntas frecuentes

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

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