5500 W Buffer E is needed for ligation sequencing on the 5500 W Series genetic analyzers. Supplemental buffers like this one can be used to replace components of the main buffer kit. 5500 W Buffer E consists of 5500 W Buffer E Part 1 and 5500 W Buffer E Part 2, which need to be mixed prior to running on the instrument. 5500 W Buffer E contains sufficient reagents to run up to 432 cycles, or 72 cycles per lane for 6 lanes.