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Ion Torrent™
Ion Library Equalizer™ Kit
The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and libraryMás información
| Número de catálogo | Cantidad |
|---|---|
| 4482298 | 96 reacciones |
Número de catálogo 4482298
Precio (MXN)
-
Cantidad:
96 reacciones
The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects.
The kit workflow consists of three simple steps:
1) Library amplification with Equalizer™ Primers
2) Library capture onto Equalizer™ Beads
3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer
Simple Workflow
• Seamlessly integrates into your existing library preparation workflow
• Reduced pipetting compared to the Agilent 2100 Bioanalyzer™-based workflow
• Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries
• Minimizes library-to-library variability
Cost Effective
• No additional capital equipment required
• Less expensive than Agilent 2100 Bioanalyzer™-based workflow (up to 10 samples)
• Reduced AMPure™ XP reagent usage compared to Agilent 2100 Bioanalyzer™-based workflow
• Long shelf life: up to 18 months
• Convenient 96-reaction kit helps reduce ordering frequency
Flexible
• Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors
• Use with a single sample or in a 96-well plate format
• Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization
Includes:
The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions.
Required for Workflow:
Ion AmpliSeq™ Library Kit 2.0
Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com)
--OR--
Ion Plus Fragment Library Kit
--OR--
Ion Xpress™ Plus Fragment Library Kit
Optional:
Ion Xpress™ Barcode Adapters Kits
The kit workflow consists of three simple steps:
1) Library amplification with Equalizer™ Primers
2) Library capture onto Equalizer™ Beads
3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer
Simple Workflow
• Seamlessly integrates into your existing library preparation workflow
• Reduced pipetting compared to the Agilent 2100 Bioanalyzer™-based workflow
• Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries
• Minimizes library-to-library variability
Cost Effective
• No additional capital equipment required
• Less expensive than Agilent 2100 Bioanalyzer™-based workflow (up to 10 samples)
• Reduced AMPure™ XP reagent usage compared to Agilent 2100 Bioanalyzer™-based workflow
• Long shelf life: up to 18 months
• Convenient 96-reaction kit helps reduce ordering frequency
Flexible
• Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors
• Use with a single sample or in a 96-well plate format
• Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization
Includes:
The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions.
Required for Workflow:
Ion AmpliSeq™ Library Kit 2.0
Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com)
--OR--
Ion Plus Fragment Library Kit
--OR--
Ion Xpress™ Plus Fragment Library Kit
Optional:
Ion Xpress™ Barcode Adapters Kits
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Para utilizar con (aplicación)Amplicon Sequencing
Para utilizar con (equipo)Ion PGM™ System, Ion Proton™ System, Ion Personal Genome Machine (PGM™) System
Formato96-well Plate
BibliotecasFragment Library, Targeted Sequencing Library, Normalized Library
Capacidad multiplexNo Limit on Multiplexing
Tipo de productoLibrary Equalizer Kit
Cantidad96 reacciones
Tipo de muestraAny Sample Type
Volumen de muestraFragment or Targeted AmpliSeq Libraries
Tipo de secuenciaciónEpigenetic Sequencing, Genome & DNA Sequencing
EspecieAll Species
Capacidad de procesamientoUp to 10 Samples
Paso del flujo de trabajoLibrary Barcoding, Library Generation, Template Preparation
Línea de productosEqualizer
Material de partidaADN
Unit SizeEach
Contenido y almacenamiento
• Equalizer Primers - store at 2–8°C
• Equalizer Capture - store at 2–8°C
• Equalizer Wash Buffer - store at room temperature (can be stored at 2–8°C)
• Equalizer Elution Buffer - store at 2–8°C
• Equalizer Beads - store at 2–8°C
Note kits are shipped at ambient temperature store each component at specified temperatures.
• Equalizer Capture - store at 2–8°C
• Equalizer Wash Buffer - store at room temperature (can be stored at 2–8°C)
• Equalizer Elution Buffer - store at 2–8°C
• Equalizer Beads - store at 2–8°C
Note kits are shipped at ambient temperature store each component at specified temperatures.